Sigworth Lab Publications

• Cryo-EM structure of the brine shrimp mitochondrial ATP synthase suggests an inactivation mechanism for the ATP synthase leak channel.Kumar A, da Fonseca Rezende E Mello J, Wu Y, Morris D, Mezghani I, Smith E, Rombauts S, Bossier P, Krahn J, Sigworth FJ, Mnatsakanyan N. Cell Death Differ. 2025 Mar 19; 2025 Mar 19. PMID: 40108410.
• CryoEM structures of Kv1.2 potassium channels, conducting and non-conducting.Wu Y, Yan Y, Yang Y, Bian S, Rivetta A, Allen K, Sigworth FJ. Elife. 2025 Feb 13; 2025 Feb 13. PMID: 39945513.
• REliable PIcking by Consensus (REPIC): a consensus methodology for harnessing multiple cryo-EM particle pickers.Cameron CJF, Seager SJH, Sigworth FJ, Tagare HD, Gerstein MB. Commun Biol. 2024 Oct 31; 2024 Oct 31. PMID: 39482410.
• Cryo-EM structures of Kv1.2 potassium channels, conducting and non-conducting.Wu Y, Yan Y, Yang Y, Bian S, Rivetta A, Allen K, Sigworth FJ. bioRxiv. 2024 Aug 10; 2024 Aug 10. PMID: 37398110.
• Methods for Cryo-EM Single Particle Reconstruction of Macromolecules Having Continuous Heterogeneity.Toader B, Sigworth FJ, Lederman RR. J Mol Biol. 2023 May 1; 2023 Feb 28. PMID: 36863660.
• The sensor for animal electricity.Sigworth FJ. Proc Natl Acad Sci U S A. 2023 Jan 3; 2022 Dec 27. PMID: 36574669.
• Structural insights into the mechanism of the sodium/iodide symporter.Ravera S, Nicola JP, Salazar-De Simone G, Sigworth FJ, Karakas E, Amzel LM, Bianchet MA, Carrasco N. Nature. 2022 Dec; 2022 Dec 14. PMID: 36517601.
• Structure and analysis of nanobody binding to the human ASIC1a ion channel.Wu Y, Chen Z, Sigworth FJ, Canessa CM. Elife. 2021 Jul 28; 2021 Jul 28. PMID: 34319232.
• Computational Methods for Single-Particle Electron Cryomicroscopy.Singer A, Sigworth FJ. Annu Rev Biomed Data Sci. 2020 Jul; 2020 May 4. PMID: 34485850.
• A mitochondrial megachannel resides in monomeric F(1)F(O) ATP synthase.Mnatsakanyan N, Llaguno MC, Yang Y, Yan Y, Weber J, Sigworth FJ, Jonas EA. Nat Commun. 2019 Dec 20; 2019 Dec 20. PMID: 31862883.
• Current carried by the Slc26 family member prestin does not flow through the transporter pathway.Bai JP, Moeini-Naghani I, Zhong S, Li FY, Bian S, Sigworth FJ, Santos-Sacchi J, Navaratnam D. Sci Rep. 2017 Apr 19; 2017 Apr 19. PMID: 28422190.
• Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins.Jensen KH, Brandt SS, Shigematsu H, Sigworth FJ. J Struct Biol. 2016 Apr; 2016 Feb 1. PMID: 26835990.
• Principles of cryo-EM single-particle image processing.Sigworth FJ. Microscopy (Oxf). 2016 Feb; 2015 Dec 24. PMID: 26705325.
• Cryo-EM: Spinning the Micelles Away.Singh SK, Sigworth FJ. Structure. 2015 Sep 1. PMID: 26331455.
• Directly reconstructing principal components of heterogeneous particles from cryo-EM images.Tagare HD, Kucukelbir A, Sigworth FJ, Wang H, Rao M. J Struct Biol. 2015 Aug; 2015 Jun 4. PMID: 26049077.
• SubspaceEM: A fast maximum-a-posteriori algorithm for cryo-EM single particle reconstruction.Dvornek NC, Sigworth FJ, Tagare HD. J Struct Biol. 2015 May; 2015 Mar 31. PMID: 25839831.
• Automatic cryo-EM particle selection for membrane proteins in spherical liposomes.Liu Y, Sigworth FJ. J Struct Biol. 2014 Mar; 2014 Jan 24. PMID: 24468290.
• Quantifying the local resolution of cryo-EM density maps.Kucukelbir A, Sigworth FJ, Tagare HD. Nat Methods. 2014 Jan; 2013 Nov 10. PMID: 24213166.
• Expression, purification and functional reconstitution of slack sodium-activated potassium channels.Yan Y, Yang Y, Bian S, Sigworth FJ. J Membr Biol. 2012 Nov; 2012 Jun 23. PMID: 22729647.
• Hydration-layer models for cryo-EM image simulation.Shang Z, Sigworth FJ. J Struct Biol. 2012 Oct; 2012 May 17. PMID: 22609687.
• A Bayesian adaptive basis algorithm for single particle reconstruction.Kucukelbir A, Sigworth FJ, Tagare HD. J Struct Biol. 2012 Jul; 2012 May 1. PMID: 22564910.
• Surface-Constrained 3D Reconstruction in Cryo-EM.Barthel AC, Tagare H, Sigworth FJ. Conf Rec Asilomar Conf Signals Syst Comput. 2011. PMID: 24477184.
• Interactions between β-catenin and the HSlo potassium channel regulates HSlo surface expression.Bian S, Bai JP, Chapin H, Le Moellic C, Dong H, Caplan M, Sigworth FJ, Navaratnam DS. PLoS One. 2011; 2011 Dec 14. PMID: 22194818.
• Improved hidden Markov models for molecular motors, part 1: basic theory.Müllner FE, Syed S, Selvin PR, Sigworth FJ. Biophys J. 2010 Dec 1. PMID: 21112293.
• Patch-clamp amplifiers on a chip.Weerakoon P, Culurciello E, Yang Y, Santos-Sacchi J, Kindlmann PJ, Sigworth FJ. J Neurosci Methods. 2010 Oct 15; 2010 Jul 15. PMID: 20637803.
• An adaptive Expectation-Maximization algorithm with GPU implementation for electron cryomicroscopy.Tagare HD, Barthel A, Sigworth FJ. J Struct Biol. 2010 Sep; 2010 Jun 9. PMID: 20538058.
• Fragile X mental retardation protein controls gating of the sodium-activated potassium channel Slack.Brown MR, Kronengold J, Gazula VR, Chen Y, Strumbos JG, Sigworth FJ, Navaratnam D, Kaczmarek LK. Nat Neurosci. 2010 Jul; 2010 May 30. PMID: 20512134.
• Reference Free Structure Determination through Eigenvectors of Center of Mass Operators.Coifman RR, Shkolnisky Y, Sigworth FJ, Singer A. Appl Comput Harmon Anal. 2010 May. PMID: 20436952.
• An introduction to maximum-likelihood methods in cryo-EM.Sigworth FJ, Doerschuk PC, Carazo JM, Scheres SH. Methods Enzymol. 2010. PMID: 20888965.
• Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM.Wang L, Sigworth FJ. Methods Enzymol. 2010. PMID: 20887857.
• Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy.Wang L, Sigworth FJ. Nature. 2009 Sep 10; 2009 Aug 30. PMID: 19718020.
• Streptavidin crystals as nanostructured supports and image-calibration references for cryo-EM data collection.Wang L, Ounjai P, Sigworth FJ. J Struct Biol. 2008 Nov; 2008 Aug 5. PMID: 18707004.
• Graph Laplacian tomography from unknown random projections.Coifman RR, Shkolnisky Y, Sigworth FJ, Singer A. IEEE Trans Image Process. 2008 Oct. PMID: 18784036.
• Importance of the Debye screening length on nanowire field effect transistor sensors.Stern E, Wagner R, Sigworth FJ, Breaker R, Fahmy TM, Reed MA. Nano Lett. 2007 Nov; 2007 Oct 3. PMID: 17914853.
• The last few frames of the voltage-gating movie.Sigworth FJ. Biophys J. 2007 Nov 1; 2007 Aug 17. PMID: 17704178.
• Two conformational states of the membrane-associated Bacillus thuringiensis Cry4Ba delta-endotoxin complex revealed by electron crystallography: implications for toxin-pore formation.Ounjai P, Unger VM, Sigworth FJ, Angsuthanasombat C. Biochem Biophys Res Commun. 2007 Oct 5; 2007 Jul 25. PMID: 17681273.
• Holey carbon micro-arrays for transmission electron microscopy: a microcontact printing approach.Chester DW, Klemic JF, Stern E, Sigworth FJ, Klemic KG. Ultramicroscopy. 2007 Aug; 2007 Jan 30. PMID: 17331648.
• Using cryo-EM to measure the dipole potential of a lipid membrane.Wang L, Bose PS, Sigworth FJ. Proc Natl Acad Sci U S A. 2006 Dec 5; 2006 Nov 20. PMID: 17116859.
• Pharmacological activation and inhibition of Slack (Slo2.2) channels.Yang B, Gribkoff VK, Pan J, Damagnez V, Dworetzky SI, Boissard CG, Bhattacharjee A, Yan Y, Sigworth FJ, Kaczmarek LK. Neuropharmacology. 2006 Sep; 2006 Jul 28. PMID: 16876206.
• Microfluidic system for planar patch clamp electrode arrays.Li X, Klemic KG, Reed MA, Sigworth FJ. Nano Lett. 2006 Apr. PMID: 16608289.
• Cryo-EM and single particles.Wang L, Sigworth FJ. Physiology (Bethesda). 2006 Feb. PMID: 16443818.
• Gating of acid-sensitive ion channel-1: release of Ca2+ block vs. allosteric mechanism.Zhang P, Sigworth FJ, Canessa CM. J Gen Physiol. 2006 Feb; 2006 Jan 17. PMID: 16418400.
• Generation of functional fluorescent BK channels by random insertion of GFP variants.Giraldez T, Hughes TE, Sigworth FJ. J Gen Physiol. 2005 Nov. PMID: 16260837.
• Microchip technology in ion-channel research.Sigworth FJ, Klemic KG. IEEE Trans Nanobioscience. 2005 Mar. PMID: 15816178.
• An air-molding technique for fabricating PDMS planar patch-clamp electrodes.Klemic KG, Klemic JF, Sigworth FJ. Pflugers Arch. 2005 Mar; 2004 Dec 1. PMID: 15578213.
• Can Shaker potassium channels be locked in the deactivated state?Yang Y, Yan Y, Sigworth FJ. J Gen Physiol. 2004 Aug. PMID: 15277577.
• Classical detection theory and the cryo-EM particle selection problem.Sigworth FJ. J Struct Biol. 2004 Jan-Feb. PMID: 15065679.
• Slick (Slo2.1), a rapidly-gating sodium-activated potassium channel inhibited by ATP.Bhattacharjee A, Joiner WJ, Wu M, Yang Y, Sigworth FJ, Kaczmarek LK. J Neurosci. 2003 Dec 17. PMID: 14684870.
• Three-dimensional structure of the type 1 inositol 1,4,5-trisphosphate receptor at 24 A resolution.Jiang QX, Thrower EC, Chester DW, Ehrlich BE, Sigworth FJ. EMBO J. 2002 Jul 15. PMID: 12110570.
• Patch clamp on a chip.Sigworth FJ, Klemic KG. Biophys J. 2002 Jun. PMID: 12023207.
• Micromolded PDMS planar electrode allows patch clamp electrical recordings from cells.Klemic KG, Klemic JF, Reed MA, Sigworth FJ. Biosens Bioelectron. 2002 Jun. PMID: 11959483.