2000
The HMG-CoA reductase inhibitor simvastatin activates the protein kinase Akt and promotes angiogenesis in normocholesterolemic animals.
Kureishi Y, Luo Z, Shiojima I, Bialik A, Fulton D, Lefer D, Sessa W, Walsh K. The HMG-CoA reductase inhibitor simvastatin activates the protein kinase Akt and promotes angiogenesis in normocholesterolemic animals. Nature Medicine 2000, 6: 1004-1010. PMID: 10973320, PMCID: PMC2828689, DOI: 10.1038/79510.Peer-Reviewed Original ResearchConceptsProtein kinase Akt/PKBKinase Akt/PKBProtein kinase AktAkt/PKBAkt-dependent mannerVascular structure formationActivation of AktKinase AktVascular endothelial growth factor treatmentEnhanced phosphorylationBlood vessel growthNew blood vessel growthAktGrowth factor treatmentVessel growthEndothelial cellsEndothelial nitric oxide synthaseRecent studiesHMG-CoA reductase inhibitor simvastatinAngiogenesisPKBFactor treatmentPhosphorylationReductase inhibitor simvastatinApoptosisAcute modulation of endothelial Akt/PKB activity alters nitric oxide–dependent vasomotor activity in vivo
Luo Z, Fujio Y, Kureishi Y, Rudic R, Daumerie G, Fulton D, Sessa W, Walsh K. Acute modulation of endothelial Akt/PKB activity alters nitric oxide–dependent vasomotor activity in vivo. Journal Of Clinical Investigation 2000, 106: 493-499. PMID: 10953024, PMCID: PMC380252, DOI: 10.1172/jci9419.Peer-Reviewed Original ResearchConceptsDN-AktEndothelial cell nitric oxide synthaseMyr-AktSerine/threonine protein kinase AktProtein kinase AktDominant-negative AktNitric oxideVasomotor toneFemoral arteryAkt functionReplication-defective adenoviral constructKinase AktActive AktEndothelium-dependent vasodilatationKey regulatorEndothelium-independent vasodilatorEndothelium-dependent vasomotionRabbit femoral artery modelNitric oxide synthaseAorta ex vivoImportant regulatorGene transferDoppler flow measurementsAktENOS inhibitor
1993
20-Hydroxyeicosatetraenoic acid is an endothelium-dependent vasoconstrictor in rabbit arteries
Escalante B, Omata K, Sessa W, Lee S, Falck J, Schwartzman M. 20-Hydroxyeicosatetraenoic acid is an endothelium-dependent vasoconstrictor in rabbit arteries. European Journal Of Pharmacology 1993, 235: 1-7. PMID: 8519270, DOI: 10.1016/0014-2999(93)90812-v.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicArachidonic AcidCarotid ArteriesCattleCells, CulturedDose-Response Relationship, DrugEndothelium, VascularHydroxyeicosatetraenoic AcidsIn Vitro TechniquesMaleMesenteric ArteriesProstaglandin-Endoperoxide SynthasesRabbitsRenal ArteryVasoconstrictionVasoconstrictor AgentsConceptsBlood vesselsEndothelium-dependent vasoconstrictorEndothelial cyclooxygenaseRegulation of hemostasisVasoconstrictor metabolitesContractile potencyContractile effectAortic ringsVascular tonePlatelet functionMuscular arteriesRabbit arteriesArachidonate metabolitesPlatelet aggregationCyclooxygenaseArachidonic acidArteryBlood cellsRabbit kidneySeminal vesiclesEicosatetraenoic acidMajor arachidonate metabolitePotencyRam seminal vesiclesPresent study
1991
Human Polymorphonuclear Leukocytes Generate and Degrade Endothelin-1 by Two Distinct Neutral Proteases
Sessa W, Kaw S, Zembowicz A, Änggård E, Hecker M, Vane J. Human Polymorphonuclear Leukocytes Generate and Degrade Endothelin-1 by Two Distinct Neutral Proteases. Journal Of Cardiovascular Pharmacology 1991, 17: s34-38. PMID: 1725374, DOI: 10.1097/00005344-199100177-00010.Peer-Reviewed Original ResearchConceptsET-1Big endothelinEndothelin-1Important pathophysiologic implicationsHuman polymorphonuclear leukocytesFMLP-activated PMNHuman big endothelinContractile factorsPMN infiltrationLeukocyte cathepsin GContractile activityPathophysiologic implicationsActivated PMNsPolymorphonuclear leukocytesNeutral proteaseDisease statesCell-free supernatantCathepsin GPMNPhosphoramidonMetabolismThe biosynthesis of endothelin-1 by human polymorphonuclear leukocytes
Sessa W, Kaw S, Hecker M, Vane J. The biosynthesis of endothelin-1 by human polymorphonuclear leukocytes. Biochemical And Biophysical Research Communications 1991, 174: 613-618. PMID: 1993057, DOI: 10.1016/0006-291x(91)91461-k.Peer-Reviewed Original Research
1990
L-Glutamine inhibits the release of endothelium-derived relaxing factor from the rabbit aorta
Swierkosz T, Mitchell J, Sessa W, Hecker M, Vane J. L-Glutamine inhibits the release of endothelium-derived relaxing factor from the rabbit aorta. Biochemical And Biophysical Research Communications 1990, 172: 143-148. PMID: 2222463, DOI: 10.1016/s0006-291x(05)80184-6.Peer-Reviewed Original ResearchConceptsRelease of EDRFRabbit aortic stripsAortic stripsRabbit aortaL-arginineL-ArgACh-induced relaxationEndothelium-dependent relaxationL-GlnL-Arg levelsIntact blood vesselsEDRF biosynthesisAortic tissueConsecutive infusionsEDRFL-glutamineAortaEndothelial cellsInitial equilibration periodBlood vesselsD-ArgInhibitory effectInfusionPresent studyAmino acid L-glutamineRelease of a neutrophil‐derived vascoconstrictor agent which augments platelet‐induced contractions of blood vessels in vitro
Sessa W, Mullane K. Release of a neutrophil‐derived vascoconstrictor agent which augments platelet‐induced contractions of blood vessels in vitro. British Journal Of Pharmacology 1990, 99: 553-559. PMID: 2110018, PMCID: PMC1917347, DOI: 10.1111/j.1476-5381.1990.tb12967.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicBlood PlateletsCalcimycinIn Vitro TechniquesLipoxygenase InhibitorsMaleMolecular WeightMuscle ContractionMuscle TonusMuscle, Smooth, VascularNeutrophilsN-Formylmethionine Leucyl-PhenylalanineRabbitsSerotoninSerotonin AntagonistsTetradecanoylphorbol AcetateVasoconstrictor AgentsConceptsContractile factorsVascular toneBlood vesselsEnd-organ antagonistNeutrophil-derived productsPlatelet-induced contractionsConcentration-dependent contractionsInhibition of contractionRat stomach stripCyclo-oxygenase enzymeTimes more plateletsRabbit thoracic aortaPhorbol myristate acetateFree radical scavengerContractile responseInhibitors of lipoxygenaseOrgan bathAngiotensin IIReceptor antagonistStomach stripIntact endotheliumNeutrophil activationThoracic aortaActivated neutrophilsDesArg9-bradykinin
1987
Myocardial Salvage Induced by REV-5901
Mullane K, Hatala M, Kraemer R, Sessa W, Westlin W. Myocardial Salvage Induced by REV-5901. Journal Of Cardiovascular Pharmacology 1987, 10: 398-406. PMID: 2444792, DOI: 10.1097/00005344-198710000-00004.Peer-Reviewed Original ResearchConceptsCoronary artery occlusionArtery occlusionInfarct sizeEnd-organ antagonistLeukotriene-like materialAccumulation of neutrophilsLeukotriene B4 generationSelective cyclooxygenase inhibitorsLeukotriene B4 formationSpasmogenic effectNeutrophil accumulationHemodynamic effectsHypoperfused zoneMyocardial injuryMyocardial salvageCardiac damageIschemic myocardiumB4 generationAnesthetized dogsCyclooxygenase inhibitorIschemic heartB4 formationCyclooxygenase enzymesCanine neutrophilsLipoxygenase metabolites