2020
Structural elucidation of the cis-prenyltransferase NgBR/DHDDS complex reveals insights in regulation of protein glycosylation
Edani BH, Grabińska KA, Zhang R, Park EJ, Siciliano B, Surmacz L, Ha Y, Sessa WC. Structural elucidation of the cis-prenyltransferase NgBR/DHDDS complex reveals insights in regulation of protein glycosylation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 20794-20802. PMID: 32817466, PMCID: PMC7456142, DOI: 10.1073/pnas.2008381117.Peer-Reviewed Original ResearchConceptsActive site tunnelProtein glycosylationAtomic resolution structuresGlycosyl carrier lipidsΑ3 helixEnzyme active sitePTase activityResolution structureActive siteEndoplasmic reticulumHomodimeric formCarrier lipidRate-limiting stepGlycosylationCrystal structureDHDDSStructural elucidationPTaseIsoprene chainPrenyltransferaseUnique insightsComplexesUnfavorable stateNgBRHomodimeric
2017
Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae
Hoffmann R, Grabińska K, Guan Z, Sessa WC, Neiman AM. Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae. Genetics 2017, 207: 1371-1386. PMID: 28978675, PMCID: PMC5714454, DOI: 10.1534/genetics.117.300322.Peer-Reviewed Original ResearchConceptsLong-chain polyprenolsSpore wallSynthesis of chitinSpore wall layerHaploid genomeCellular processesProtein glycosylationPrenyltransferase activitySporulating cellsWall formationDityrosine layerIsoprenoid lipidsChitin synthaseEndoplasmic reticulumVegetative cellsSRT1Lipid dropletsDividing cellsEssential precursorPrimary enzymeImportant functionsSaccharomycesPolyprenolsDolicholSugar carrier
2009
The YTA7 gene is involved in the regulation of the isoprenoid pathway in the yeast Saccharomyces cerevisiae
Kuranda K, Grabinska K, Berges T, Karst F, Leberre V, Sokol S, François J, Palamarczyk G. The YTA7 gene is involved in the regulation of the isoprenoid pathway in the yeast Saccharomyces cerevisiae. FEMS Yeast Research 2009, 9: 381-390. PMID: 19416104, DOI: 10.1111/j.1567-1364.2009.00485.x.Peer-Reviewed Original ResearchConceptsIsoprenoid pathwayFPP synthaseTwo-hybrid screenMembrane-associated proteinsBiosynthesis of hemeIsoprenoid biosynthesisProtein partnersPresence of lovastatinYeast SaccharomycesN-glycosylationSterol biosynthesisSqualene synthaseCis-prenyltransferaseEndoplasmic reticulumIsoprenoid compoundsZaragozic acidsBiosynthesisCellular levelGenesEnzymatic activityExpression levelsSaccharomycesPathwaySynthaseRegulation