2010
Dendritic Cells Transduced With Lentiviral Vectors Expressing VIP Differentiate Into VIP-secreting Tolerogenic-like DCs
Toscano M, Delgado M, Kong W, Martin F, Skarica M, Ganea D. Dendritic Cells Transduced With Lentiviral Vectors Expressing VIP Differentiate Into VIP-secreting Tolerogenic-like DCs. Molecular Therapy 2010, 18: 1035-1045. PMID: 20068554, PMCID: PMC2890107, DOI: 10.1038/mt.2009.293.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Marrow CellsCell DifferentiationCell MovementCells, CulturedDendritic CellsEnzyme-Linked Immunosorbent AssayFlow CytometryGenetic VectorsLentivirusMiceMice, Inbred BALB CPolymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionTransduction, GeneticVasoactive Intestinal PeptideConceptsVasoactive intestinal peptideExperimental autoimmune encephalomyelitisDendritic cellsTherapeutic effectTolerogenic DCsVIP administrationInflammatory disordersT cellsImmune responseBone marrow-derived dendritic cellsBone marrow-derived DCsMarrow-derived dendritic cellsNeuropeptide vasoactive intestinal peptideTolerogenic dendritic cellsAllogeneic T cellsAutoimmune/inflammatory disordersRegulatory T cellsIL-10 productionProinflammatory cytokine secretionPromising therapeutic toolLentiviral vectorsHigh-dose inoculationCLP miceAutoimmune encephalomyelitisPuncture (CLP) sepsis
2009
Cyclophosphamide resets dendritic cell homeostasis and enhances antitumor immunity through effects that extend beyond regulatory T cell elimination
Radojcic V, Bezak K, Skarica M, Pletneva M, Yoshimura K, Schulick R, Luznik L. Cyclophosphamide resets dendritic cell homeostasis and enhances antitumor immunity through effects that extend beyond regulatory T cell elimination. Cancer Immunology, Immunotherapy 2009, 59: 137-148. PMID: 19590872, PMCID: PMC3103867, DOI: 10.1007/s00262-009-0734-3.Peer-Reviewed Original ResearchConceptsDendritic cell homeostasisAntitumor immunityT cellsLow-dose total body irradiationTumor antigen-specific T cellsAnti-CD25 monoclonal antibodyHematopoietic stem cell supportAntigen-specific T cellsUntreated tumor-bearing animalsTumor-infiltrating DCsExpansion of TregsRegulatory T cellsStem cell supportTotal body irradiationT cell responsesLess IL-10More IL-12Tumor-bearing animalsT cell eliminationCell homeostasisDC turnoverTreg depletionImmunotherapeutic strategiesIL-10Body irradiation