Publications
2023
- The release of inhibition model reproduces kinetics and plasticity of neurotransmitter release in central synapses.Norman CA, Krishnakumar SS, Timofeeva Y, Volynski KE. Commun Biol. 2023 Oct 27; 2023 Oct 27. PMID: 37891212.
- Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery.Kalyana Sundaram RV, Chatterjee A, Bera M, Grushin K, Panda A, Li F, Coleman J, Lee S, Ramakrishnan S, Ernst AM, Gupta K, Rothman JE, Krishnakumar SS. Proc Natl Acad Sci U S A. 2023 Aug 22; 2023 Aug 17. PMID: 37590407.
- Novel Roles for Diacylglycerol in Synaptic Vesicle Priming and Release Revealed by Complete Reconstitution of Core Protein Machinery.Sundaram RVK, Chatterjee A, Bera M, Grushin K, Panda A, Li F, Coleman J, Lee S, Ramakrishnan S, Ernst AM, Gupta K, Rothman JE, Krishnakumar SS. bioRxiv. 2023 Jun 7; 2023 Jun 7. PMID: 37333317.
- Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer.Panda A, Giska F, Duncan AL, Welch AJ, Brown C, McAllister R, Hariharan P, Goder JND, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman JE, Gupta K. Nat Methods. 2023 Jun; 2023 Apr 27. PMID: 37106230.
- The release of inhibition model reproduces kinetics and plasticity of neurotransmitter release in central synapses.Norman CA, Krishnakumar SS, Timofeeva Y, Volynski KE. Res Sq. 2023 Apr 27; 2023 Apr 27. PMID: 37163032.
2022
- Native Planar Asymmetric Suspended Membrane for Single-Molecule Investigations: Plasma Membrane on a Chip.Kalyana Sundaram RV, Bera M, Coleman J, Weerakkody JS, Krishnakumar SS, Ramakrishnan S. Small. 2022 Dec; 2022 Nov 3. PMID: 36328714.
- Synaptotagmin rings as high-sensitivity regulators of synaptic vesicle docking and fusion.Zhu J, McDargh ZA, Li F, Krishnakumar SS, Rothman JE, O'Shaughnessy B. Proc Natl Acad Sci U S A. 2022 Sep 20; 2022 Sep 14. PMID: 36103579.
- Molecular determinants of complexin clamping and activation function.Bera M, Ramakrishnan S, Coleman J, Krishnakumar SS, Rothman JE. Elife. 2022 Apr 20; 2022 Apr 20. PMID: 35442188.
2021
- Vesicle capture by membrane-bound Munc13-1 requires self-assembly into discrete clusters.Li F, Kalyana Sundaram RV, Gatta AT, Coleman J, Ramakrishnan S, Krishnakumar SS, Pincet F, Rothman JE. FEBS Lett. 2021 Sep; 2021 Jul 24. PMID: 34227103.
- Symmetrical arrangement of proteins under release-ready vesicles in presynaptic terminals.Radhakrishnan A, Li X, Grushin K, Krishnakumar SS, Liu J, Rothman JE. Proc Natl Acad Sci U S A. 2021 Feb 2. PMID: 33468631.
- Munc13 binds and recruits SNAP25 to chaperone SNARE complex assembly.Kalyana Sundaram RV, Jin H, Li F, Shu T, Coleman J, Yang J, Pincet F, Zhang Y, Rothman JE, Krishnakumar SS. FEBS Lett. 2021 Feb; 2020 Dec 5. PMID: 33222163.
2020
- Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain.Gruget C, Bello O, Coleman J, Krishnakumar SS, Perez E, Rothman JE, Pincet F, Donaldson SH Jr. Sci Rep. 2020 Oct 22; 2020 Oct 22. PMID: 33093513.
- Synergistic roles of Synaptotagmin-1 and complexin in calcium-regulated neuronal exocytosis.Ramakrishnan S, Bera M, Coleman J, Rothman JE, Krishnakumar SS. Elife. 2020 May 13; 2020 May 13. PMID: 32401194.
- Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release.Tagliatti E, Bello OD, Mendonça PRF, Kotzadimitriou D, Nicholson E, Coleman J, Timofeeva Y, Rothman JE, Krishnakumar SS, Volynski KE. Proc Natl Acad Sci U S A. 2020 Feb 18; 2020 Feb 3. PMID: 32015138.
2019
- Mechanisms of Neurological Dysfunction in GOSR2 Progressive Myoclonus Epilepsy, a Golgi SNAREopathy.Jepson JEC, Praschberger R, Krishnakumar SS. Neuroscience. 2019 Nov 10; 2019 Apr 4. PMID: 30954670.
- Structural basis for the clamping and Ca(2+) activation of SNARE-mediated fusion by synaptotagmin.Grushin K, Wang J, Coleman J, Rothman JE, Sindelar CV, Krishnakumar SS. Nat Commun. 2019 Jun 3; 2019 Jun 3. PMID: 31160571.
- Mutations in the Neuronal Vesicular SNARE VAMP2 Affect Synaptic Membrane Fusion and Impair Human Neurodevelopment.Salpietro V, Malintan NT, Llano-Rivas I, Spaeth CG, Efthymiou S, Striano P, Vandrovcova J, Cutrupi MC, Chimenz R, David E, Di Rosa G, Marce-Grau A, Raspall-Chaure M, Martin-Hernandez E, Zara F, Minetti C, Bello OD, De Zorzi R, Fortuna S, Dauber A, Alkhawaja M, Sultan T, Mankad K, Vitobello A, Thomas Q, Mau-Them FT, Faivre L, Martinez-Azorin F, Prada CE, Macaya A, Kullmann DM, Rothman JE, Krishnakumar SS, Houlden H. Am J Hum Genet. 2019 Apr 4; 2019 Mar 28. PMID: 30929742.
- Synaptotagmin oligomers are necessary and can be sufficient to form a Ca(2+) -sensitive fusion clamp.Ramakrishnan S, Bera M, Coleman J, Krishnakumar SS, Pincet F, Rothman JE. FEBS Lett. 2019 Jan; 2019 Jan 18. PMID: 30570144.
- Symmetrical organization of proteins under docked synaptic vesicles.Li X, Radhakrishnan A, Grushin K, Kasula R, Chaudhuri A, Gomathinayagam S, Krishnakumar SS, Liu J, Rothman JE. FEBS Lett. 2019 Jan; 2019 Jan 18. PMID: 30561792.
- Using Nanodiscs to Probe Ca(2+)-Dependent Membrane Interaction of Synaptotagmin-1.Stroeva E, Krishnakumar SS. Methods Mol Biol. 2019. PMID: 30317508.
2018
- Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS. Proc Natl Acad Sci U S A. 2018 Aug 7; 2018 Jul 23. PMID: 30038018.
- Synergistic control of neurotransmitter release by different members of the synaptotagmin family.Volynski KE, Krishnakumar SS. Curr Opin Neurobiol. 2018 Aug; 2018 Jun 7. PMID: 29886350.
- Rearrangements under confinement lead to increased binding energy of Synaptotagmin-1 with anionic membranes in Mg(2+) and Ca(2).Gruget C, Coleman J, Bello O, Krishnakumar SS, Perez E, Rothman JE, Pincet F, Donaldson SH Jr. FEBS Lett. 2018 May; 2018 Apr 10. PMID: 29578584.
- Two Disease-Causing SNAP-25B Mutations Selectively Impair SNARE C-terminal Assembly.Rebane AA, Wang B, Ma L, Qu H, Coleman J, Krishnakumar S, Rothman JE, Zhang Y. J Mol Biol. 2018 Feb 16; 2017 Oct 19. PMID: 29056461.
- PRRT2 Regulates Synaptic Fusion by Directly Modulating SNARE Complex Assembly.Coleman J, Jouannot O, Ramakrishnan SK, Zanetti MN, Wang J, Salpietro V, Houlden H, Rothman JE, Krishnakumar SS. Cell Rep. 2018 Jan 16. PMID: 29346777.
2017
- Otoferlin acts as a Ca(2+) sensor for vesicle fusion and vesicle pool replenishment at auditory hair cell ribbon synapses.Michalski N, Goutman JD, Auclair SM, Boutet de Monvel J, Tertrais M, Emptoz A, Parrin A, Nouaille S, Guillon M, Sachse M, Ciric D, Bahloul A, Hardelin JP, Sutton RB, Avan P, Krishnakumar SS, Rothman JE, Dulon D, Safieddine S, Petit C. Elife. 2017 Nov 7; 2017 Nov 7. PMID: 29111973.
- Hypothesis - buttressed rings assemble, clamp, and release SNAREpins for synaptic transmission.Rothman JE, Krishnakumar SS, Grushin K, Pincet F. FEBS Lett. 2017 Nov; 2017 Oct 31. PMID: 28983915.
- Mutations in Membrin/GOSR2 Reveal Stringent Secretory Pathway Demands of Dendritic Growth and Synaptic Integrity.Praschberger R, Lowe SA, Malintan NT, Giachello CNG, Patel N, Houlden H, Kullmann DM, Baines RA, Usowicz MM, Krishnakumar SS, Hodge JJL, Rothman JE, Jepson JEC. Cell Rep. 2017 Oct 3. PMID: 28978487.
- Circular oligomerization is an intrinsic property of synaptotagmin.Wang J, Li F, Bello OD, Sindelar CV, Pincet F, Krishnakumar SS, Rothman JE. Elife. 2017 Aug 29; 2017 Aug 29. PMID: 28850328.
- Homozygous mutations in VAMP1 cause a presynaptic congenital myasthenic syndrome.Salpietro V, Lin W, Delle Vedove A, Storbeck M, Liu Y, Efthymiou S, Manole A, Wiethoff S, Ye Q, Saggar A, McElreavey K, Krishnakumar SS, Pitt M, Bello OD, Rothman JE, Basel-Vanagaite L, Hubshman MW, Aharoni S, Manzur AY, Wirth B, Houlden H. Ann Neurol. 2017 Apr; 2017 Mar 29. PMID: 28253535.
- Dilation of fusion pores by crowding of SNARE proteins.Wu Z, Bello OD, Thiyagarajan S, Auclair SM, Vennekate W, Krishnakumar SS, O'Shaughnessy B, Karatekin E. Elife. 2017 Mar 27; 2017 Mar 27. PMID: 28346138.
- Kv1.1 channelopathy abolishes presynaptic spike width modulation by subthreshold somatic depolarization.Vivekananda U, Novak P, Bello OD, Korchev YE, Krishnakumar SS, Volynski KE, Kullmann DM. Proc Natl Acad Sci U S A. 2017 Feb 28; 2017 Feb 13. PMID: 28193892.
2016
- Ring-like oligomers of Synaptotagmins and related C2 domain proteins.Zanetti MN, Bello OD, Wang J, Coleman J, Cai Y, Sindelar CV, Rothman JE, Krishnakumar SS. Elife. 2016 Jul 19; 2016 Jul 19. PMID: 27434670.
- Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains.Wu Z, Auclair SM, Bello O, Vennekate W, Dudzinski NR, Krishnakumar SS, Karatekin E. Sci Rep. 2016 Jun 6; 2016 Jun 6. PMID: 27264104.
- Using ApoE Nanolipoprotein Particles To Analyze SNARE-Induced Fusion Pores.Bello OD, Auclair SM, Rothman JE, Krishnakumar SS. Langmuir. 2016 Mar 29; 2016 Mar 18. PMID: 26972604.
2015
- Re-visiting the trans insertion model for complexin clamping.Krishnakumar SS, Li F, Coleman J, Schauder CM, Kümmel D, Pincet F, Rothman JE, Reinisch KM. Elife. 2015 Apr 2; 2015 Apr 2. PMID: 25831964.
2014
- Calcium sensitive ring-like oligomers formed by synaptotagmin.Wang J, Bello O, Auclair SM, Wang J, Coleman J, Pincet F, Krishnakumar SS, Sindelar CV, Rothman JE. Proc Natl Acad Sci U S A. 2014 Sep 23; 2014 Sep 8. PMID: 25201968.
2013
- Conformational dynamics of calcium-triggered activation of fusion by synaptotagmin.Krishnakumar SS, Kümmel D, Jones SJ, Radoff DT, Reinisch KM, Rothman JE. Biophys J. 2013 Dec 3. PMID: 24314081.
2011
- Complexin cross-links prefusion SNAREs into a zigzag array.Kümmel D, Krishnakumar SS, Radoff DT, Li F, Giraudo CG, Pincet F, Rothman JE, Reinisch KM. Nat Struct Mol Biol. 2011 Jul 24; 2011 Jul 24. PMID: 21785414.
- A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusion.Krishnakumar SS, Radoff DT, Kümmel D, Giraudo CG, Li F, Khandan L, Baguley SW, Coleman J, Reinisch KM, Pincet F, Rothman JE. Nat Struct Mol Biol. 2011 Jul 24; 2011 Jul 24. PMID: 21785412.