Current Research & Projects
Stem cells are characterized by the ability to self-renew through mitotic divisions and to differentiate into a diverse range of specialized cell types to form a whole organism.
The focus of the Krause Laboratory is cell fate specification in hematopoiesis and in parathyroid development.
To better define the molecular mechanism(s) regulating hematopoiesis, we are using single cell approaches including molecular and functional analyses of highly purified primary human and murine hematopoietic subpopulations. In order to more clearly delineate the epigenetic modifications that occur during lineage commitment and correlating these changes with mRNA expression, we are using chromatin immunoprecipitation, Cut&Run, CLIP, transcriptome analysis, and high-resolution single cell microscopy including expansion microscopy. Our studies on the mechanisms of leukemogenesis are focused on how the RBM15-MKL1 fusion protein encoded by the t(1;22) translocation of AMKL promotes transformation.
Other work in the laboratory is focused on differentiation of pluripotent human cells into functional parathyroid cells.
- Hematopoiesis and Leukemogenesis using bone marrow-derived stem and progenitor cells
- Differentiation of pluripotent stem cells into functional parathyroid cells