Lab Members

The cytoplasmic domain of MxiG interacts with MxiK and directs assembly of the sorting platform in the Shigella type III secretion system.

Shoichi Tachiyama‡, Yunjie Chang§,¶, Meenakumari Muthuramalingam?, Bo Hu**, Michael L. Barta?1, Wendy L. Picking‡‡, Jun Liu§,¶2 and William D. Picking‡,‡‡3

From Fig 6: Surface rendering images of Shigella T3SS. Left) The overall architecture of T3SS is described by four main components; the cytoplasmic sorting platform (cytoplasmic side), basal body (between bacterial inner membrane, IM, and outer membrane, OM), needle (extended from the basal body), and tip complex (end of the needle). Right) Cryo-ET provided a view from IM side of T3SS. The cytoplasmic sorting platform and basal body form different numbers of symmetrical rings.

The flagellar motor is a powerful biological nanomachine that drives motility, and thus infectivity and survival, in bacteria. It is the only known molecular machine that can rotate bidirectionally – in both clockwise (CW) and counterclockwise (CCW) senses. Motor rotation relies on the passage of ions through inner membrane-embedded stator units to power the cytoplasmic switch complex (C-ring), thus generating torque. Despite decades of intensive research, the detailed mechanisms that underlie torque generation and directional switching are unclear. I joined the Liu lab to use cryo-electron tomography (cryo-ET) to study this sophisticated mechanism in the model system of the Lyme disease-causing spirochete Borrelia burgdorferi. Our high-resolution analysis of dynamic stator-C-ring interactions will reveal molecular mechanisms responsible for torque generation and rotational switching in the bacterial flagellar motor. In addition, I currently oversee the operation of our state-of-the-art cryo-focused ion beam (FIB) microscope, Aquilos2, which enables extraordinary high-throughput imaging to address diverse biological questions.

Figure: Cryo-FIB milling of the Gram-negative bacterium Proteus in swarming conditions reveals the in-situ structure of the flagellar motor. (Left) Top view of scanning electron microscopy (SEM) imag

The pathogenic bacterium Legionella pneumophila causes Legionnaires' disease by injecting a diverse array of proteins into host cells through the Dot/Icm type IV secretion system (T4SS). This elaborate nanomachine transports over 300 distinct protein effectors or toxins into eukaryotic host cells. My research focuses on utilizing high-throughput cryo-electron tomography (cryo-ET) in combination with other cutting-edge approaches to visualize and determine high-resolution 3D structures of this intricate nanomachine to reveal its transport mechanisms. This work holds great promise in advancing our understanding of T4SS macromolecular machines and their vital roles in various biological processes. Furthermore, knowledge gained from this study will open new avenues for the development of drugs and therapies combatting a wide range of infectious diseases.

Image (Jian Yue, PhD): Construction of the structural model of the Legionella pneumophila Dot/Icm T4SS, including outer membrane-associated core complex (OMCC), plug, and inner membrane-associated complex (IMC)

I am investigating host-microbe interactions mediated by bacterial secretion systems, specifically type IV (T4SS), to determine their in-situ structure and dynamics. Secretion systems play a crucial role in pathogens by translocating virulence factors from the bacterial cytoplasm to the host cell. I am now using cryo-electron microscopy and microbiology techniques to solve how these specialized nanomachines are activated to inject molecular effectors and toxins into target cells.

Image (Samira Heydari): Three-dimensional surface rendering of the outer membrane core complex (OMCC) of Cag T4SS in H.pylori, achieved using cryo-ET and subtomogram averaging.

My research focuses on the molecular mechanisms underlying host-pathogen interactions. Motor proteins play a crucial role in the assembly and function of bacterial secretion machinery and are highly conserved in various secretion systems, including type III (T3SS) and type IV (T4SS). Specifically, I am investigating the ATPase complex, a key motor protein in T3SS. My research aims to reveal the structure and exact function of this dynamic protein complex, providing valuable insights for the development of novel antibiotics.

Image (Rajeev Kumar): Cryo-ET vsualization of type IV pili (T4P) in Pseudomonas aeruginosa.