2022
Identification and Characterization of the Alternative σ28 Factor in Treponema denticola
Kurniyati K, Chang Y, Liu J, Li C. Identification and Characterization of the Alternative σ28 Factor in Treponema denticola. Journal Of Bacteriology 2022, 204: e00248-22. PMID: 36043861, PMCID: PMC9487585, DOI: 10.1128/jb.00248-22.Peer-Reviewed Original ResearchConceptsDependent promotersTranscription factorsSpirochete Treponema denticolaGene expressionOral spirochete Treponema denticolaCryo-electron tomography analysisFlagellin gene expressionExpression of flagellinSite-directed mutagenesisFlagellar regulationChemotaxis genesPeriplasmic flagellaRNA polymeraseFliABacterial motilityTranscriptional factorsLate promoterExternal flagellaLines of evidenceEscherichia coliPromoterT. denticolaBiochemical analysisEssential roleE. coli
2019
Analysis of a flagellar filament cap mutant reveals that HtrA serine protease degrades unfolded flagellin protein in the periplasm of Borrelia burgdorferi
Zhang K, Qin Z, Chang Y, Liu J, Malkowski MG, Shipa S, Li L, Qiu W, Zhang J, Li C. Analysis of a flagellar filament cap mutant reveals that HtrA serine protease degrades unfolded flagellin protein in the periplasm of Borrelia burgdorferi. Molecular Microbiology 2019, 111: 1652-1670. PMID: 30883947, PMCID: PMC6561814, DOI: 10.1111/mmi.14243.Peer-Reviewed Original ResearchConceptsPeriplasmic flagellaFliD mutantFlagellin proteinMajor flagellin proteinLyme disease spirochete Borrelia burgdorferiFlagellar cap proteinFlagellar cap protein FliDFlagellar filament assemblyFlagella-deficient mutantsMutant cellsCAP mutantsFlaB proteinsPeriplasmFilament assemblyCap proteinB. burgdorferiFlagellar filamentsGenetic studiesMutantsBorrelia burgdorferiHtrASerine proteasesFliDFlagellin polymerizationSpirochete Borrelia burgdorferi
2016
A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete
Wunder EA, Figueira CP, Benaroudj N, Hu B, Tong BA, Trajtenberg F, Liu J, Reis MG, Charon NW, Buschiazzo A, Picardeau M, Ko AI. A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete. Molecular Microbiology 2016, 101: 457-470. PMID: 27113476, PMCID: PMC4979076, DOI: 10.1111/mmi.13403.Peer-Reviewed Original ResearchConceptsTraverse tissue barriersHamster modelTarget organsSystemic infectionClinical isolatesLeptospira spirochetesTranslational motilityL. interrogansCell morphologyTissue barriersMotilityLeptospiraPeriplasmic flagellaSpirochetesVirulence phenotypesProtein AHelical cell morphologyInfectionDiseaseLeptospirosisPathogen L. interrogans