Gehlhausen Lab
My research program – working as a part of a larger group of researchers in the lab of Akiko Iwasaki, PhD – focuses on the biology of autoimmune inflammatory skin diseases. To accomplish our research, we utilize unique genetic mouse models of autoimmunity, in addition to patient samples from autoimmune skin disease patients who I see in my clinic. While our work is currently focused on lupus erythematosus, we plan to extend our observations to other relevant skin diseases like dermatomyositis that on some levels appear to have shared molecular and cellular biology. To accomplish these studies, we regularly employ a mix of both standard and newer technologies including high parameter flow cytometry, bulk RNA sequencing, single cell RNA sequencing, in addition to spatial transcriptomic analyses:
- Type I interferons and their role in autoimmune skin disease: Type I interferons are the canonical vertebrate anti-viral signaling program and are elevated in autoimmune diseases like systemic lupus erythematosus and dermatomyositis. The receptors for type I interferons are expressed broadly in nearly all cell types, though the inflammation in these diseases is often more restricted to certain organ systems like the skin. We seek to understand the influence of these antiviral programs on specific cell populations in the skin and how they mediate pathology.
- The origins of autoimmune inflammation: Though elevated antiviral signaling is well-established in autoimmune diseases like systemic lupus erythematosus, the origins of these elevations are currently unknown. We are evaluating the role of endogenous retroelements as the proximal cause of the elevated interferon signaling observed in select autoimmune diseases like lupus.
Publications
2024
- Intranasal neomycin evokes broad-spectrum antiviral immunity in the upper respiratory tract.Mao T, Kim J, Peña-Hernández MA, Valle G, Moriyama M, Luyten S, Ott IM, Gomez-Calvo ML, Gehlhausen JR, Baker E, Israelow B, Slade M, Sharma L, Liu W, Ryu C, Korde A, Lee CJ, Silva Monteiro V, Lucas C, Dong H, Yang Y, Gopinath S, Wilen CB, Palm N, Dela Cruz CS, Iwasaki A. Proc Natl Acad Sci U S A. 2024 Apr 30; 2024 Apr 22. PMID: 38648490.
- Impact of COVID-19 vaccination on symptoms and immune phenotypes in vaccine-naïve individuals with Long COVID.Grady CB, Bhattacharjee B, Silva J, Jaycox J, Lee LW, Monteiro VS, Sawano M, Massey D, Caraballo C, Gehlhausen JR, Tabachnikova A, Mao T, Lucas C, Peña-Hernandez MA, Xu L, Tzeng TJ, Takahashi T, Herrin J, Güthe DB, Akrami A, Assaf G, Davis H, Harris K, McCorkell L, Schulz WL, Grffin D, Wei H, Ring AM, Guan L, Cruz CD, Iwasaki A, Krumholz HM. medRxiv. 2024 Jan 12; 2024 Jan 12. PMID: 38260484.
- Association of psoriasis and systemic lupus erythematosus and mediation effects of tumor necrosis factor-α inhibitors: A cross-sectional study in the All of Us Research Program.Chen GF, Hong S, Ramachandran SM, Gehlhausen JR, Cohen JM. J Am Acad Dermatol. 2024 Jan; 2023 Sep 19. PMID: 37734665.
2023
- Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans.Vylegzhanina AV, Bespalov IA, Novototskaya-Vlasova KA, Hall BM, Gleiberman AS, Yu H, Leontieva OV, Leonova KI, Kurnasov OV, Osterman AL, Dy GK, Komissarov AA, Vasilieva E, Gehlhausen J, Iwasaki A, Ambrosone CB, Tsuji T, Matsuzaki J, Odunsi K, Andrianova EL, Gudkov AV. Cancer Res Commun. 2023 Nov 8. PMID: 37870410.
- Distinguishing features of long COVID identified through immune profiling.Klein J, Wood J, Jaycox JR, Dhodapkar RM, Lu P, Gehlhausen JR, Tabachnikova A, Greene K, Tabacof L, Malik AA, Silva Monteiro V, Silva J, Kamath K, Zhang M, Dhal A, Ott IM, Valle G, Peña-Hernández M, Mao T, Bhattacharjee B, Takahashi T, Lucas C, Song E, McCarthy D, Breyman E, Tosto-Mancuso J, Dai Y, Perotti E, Akduman K, Tzeng TJ, Xu L, Geraghty AC, Monje M, Yildirim I, Shon J, Medzhitov R, Lutchmansingh D, Possick JD, Kaminski N, Omer SB, Krumholz HM, Guan L, Dela Cruz CS, van Dijk D, Ring AM, Putrino D, Iwasaki A. Nature. 2023 Nov; 2023 Sep 25. PMID: 37748514.
- Vascular-Targeted Therapy for Systemic Lupus Erythematosus-Associated Vasculitis.Xu S, Patrignelli RJ, Ramachandran S, Koumpouras F, Desir D, Ko CJ, Gehlhausen JR. JAMA Dermatol. 2023 Apr 1. PMID: 36884229.
- Random skin biopsies for diagnosis of intravascular large B-cell lymphoma: Retrospective analysis of 31 biopsies from a US dermatology inpatient consultative service with literature review.Kim SR, Ko CJ, Nelson CA, Ramachandran S, Gehlhausen JR. J Am Acad Dermatol. 2023 Mar; 2022 Sep 21. PMID: 36152693.
2022
- Not all well-differentiated cutaneous squamous cell carcinomas are equal: Tumors with disparate biologic behavior have differences in protein expression via digital spatial profiling.Vesely M, Martinez-Morilla S, Gehlhausen JR, McNiff JM, Whang PG, Rimm D, Ko CJ. J Am Acad Dermatol. 2022 Sep; 2022 Apr 6. PMID: 35398219.
- Mild respiratory COVID can cause multi-lineage neural cell and myelin dysregulation.Fernández-Castañeda A, Lu P, Geraghty AC, Song E, Lee MH, Wood J, O'Dea MR, Dutton S, Shamardani K, Nwangwu K, Mancusi R, Yalçın B, Taylor KR, Acosta-Alvarez L, Malacon K, Keough MB, Ni L, Woo PJ, Contreras-Esquivel D, Toland AMS, Gehlhausen JR, Klein J, Takahashi T, Silva J, Israelow B, Lucas C, Mao T, Peña-Hernández MA, Tabachnikova A, Homer RJ, Tabacof L, Tosto-Mancuso J, Breyman E, Kontorovich A, McCarthy D, Quezado M, Vogel H, Hefti MM, Perl DP, Liddelow S, Folkerth R, Putrino D, Nath A, Iwasaki A, Monje M. Cell. 2022 Jul 7; 2022 Jun 13. PMID: 35768006.
- Lack of association between pandemic chilblains and SARS-CoV-2 infection.Gehlhausen JR, Little AJ, Ko CJ, Emmenegger M, Lucas C, Wong P, Klein J, Lu P, Mao T, Jaycox J, Wang E, Ugwu N, Muenker C, Mekael D, Klein RQ, Patrignelli R, Antaya R, McNiff J, Damsky W, Kamath K, Shon J, Ring AM, Yildirim I, Omer S, Ko AI, Aguzzi A, Iwasaki A. Proc Natl Acad Sci U S A. 2022 Mar 1. PMID: 35217624.
- Psoriasis and COVID-19: a multifactorial consideration.Cohen JM, Perez-Chada LM, De Kouchkovsky D, Gehlhausen JR. J Dermatolog Treat. 2022 Mar; 2020 Jun 22. PMID: 32568011.
- Mild respiratory SARS-CoV-2 infection can cause multi-lineage cellular dysregulation and myelin loss in the brain.Fernández-Castañeda A, Lu P, Geraghty AC, Song E, Lee MH, Wood J, Yalçın B, Taylor KR, Dutton S, Acosta-Alvarez L, Ni L, Contreras-Esquivel D, Gehlhausen JR, Klein J, Lucas C, Mao T, Silva J, Peña-Hernández MA, Tabachnikova A, Takahashi T, Tabacof L, Tosto-Mancuso J, Breyman E, Kontorovich A, McCarthy D, Quezado M, Hefti M, Perl D, Folkerth R, Putrino D, Nath A, Iwasaki A, Monje M. bioRxiv. 2022 Jan 10; 2022 Jan 10. PMID: 35043113.
2021
- A novel NFkB1 mutation linking pyoderma gangrenosum and common variable immunodeficiency.Sun Q, Gehlhausen JR, Freudzon M, Kibbi N, Bale A, Choate K, Tomayko M, Odell I, Ramachandran S. JAAD Case Rep. 2021 Dec; 2021 Oct 29. PMID: 34825039.
- Impact of circulating SARS-CoV-2 variants on mRNA vaccine-induced immunity.Lucas C, Vogels CBF, Yildirim I, Rothman JE, Lu P, Monteiro V, Gehlhausen JR, Campbell M, Silva J, Tabachnikova A, Peña-Hernandez MA, Muenker MC, Breban MI, Fauver JR, Mohanty S, Huang J, Shaw AC, Ko AI, Omer SB, Grubaugh ND, Iwasaki A. Nature. 2021 Dec; 2021 Oct 11. PMID: 34634791.
- Leukocytoclastic Vasculitis and Microvascular Occlusion: Key Concepts for the Working Pathologist.Ko CJ, Gehlhausen JR, McNiff JM. Surg Pathol Clin. 2021 Jun; 2021 Apr 28. PMID: 34023108.
- Pink plaques on the face of an adolescent male.Gehlhausen JR, Myung P, Zubek A. Int J Dermatol. 2021 Jun; 2020 Oct 20. PMID: 33084046.
- B cells join T cell clusters in the host response to recurrent herpes simplex virus 2 infection.Gehlhausen JR, Iwasaki A. J Clin Invest. 2021 May 3. PMID: 33938452.
- A detailed analysis of the distribution, morphology, and histopathology of complex purpura in hospitalized patients: A case series of 68 patients.Gehlhausen JR, Wetter DA, Nelson C, Ramachandran S, McNiff JM, Ko CJ. J Am Acad Dermatol. 2021 Apr; 2020 May 4. PMID: 32376433.
- A rapidly fluctuating rash in a stuporous patient.Ugwu N, Gehlhausen JR, Braverman IM, Bolognia JL. JAAD Case Rep. 2021 Mar; 2021 Jan 8. PMID: 33665282.
- Discordant anti-SARS-CoV-2 spike protein and RNA staining in cutaneous perniotic lesions suggests endothelial deposition of cleaved spike protein.Ko CJ, Harigopal M, Gehlhausen JR, Bosenberg M, McNiff JM, Damsky W. J Cutan Pathol. 2021 Jan; 2020 Oct 1. PMID: 32895985.
2020
- Perniosis during the COVID-19 pandemic: Negative anti-SARS-CoV-2 immunohistochemistry in six patients and comparison to perniosis before the emergence of SARS-CoV-2.Ko CJ, Harigopal M, Damsky W, Gehlhausen JR, Bosenberg M, Patrignelli R, McNiff JM. J Cutan Pathol. 2020 Nov; 2020 Sep 9. PMID: 32745281.
- A rare case of lupoid leishmaniasis defying diagnosis for a decade.Gehlhausen J, Sibindi C, Ko CJ, Grant M, Zubek A. J Cutan Pathol. 2020 Nov; 2020 Sep 6. PMID: 32623733.