2017
Enhanced Peptide Detection Toward Single-Neuron Proteomics by Reversed-Phase Fractionation Capillary Electrophoresis Mass Spectrometry
Choi S, Lombard-Banek C, Muñoz-LLancao P, Manzini M, Nemes P. Enhanced Peptide Detection Toward Single-Neuron Proteomics by Reversed-Phase Fractionation Capillary Electrophoresis Mass Spectrometry. Journal Of The American Society For Mass Spectrometry 2017, 29: 913-922. PMID: 29147852, DOI: 10.1007/s13361-017-1838-1.Peer-Reviewed Original ResearchConceptsHigh-resolution mass spectrometryReversed-phaseCapillary electrophoresisMass spectrometryTandem high-resolution mass spectrometryCapillary electrophoresis mass spectrometryRates of peptide identificationsRP fractionationCE separationProtein groupsTrace-level sensitivityRP columnDetected peptidesPeptide identificationProducts of genesSpectral interferencesProtein identificationMS/MSTechnical triplicatesCytoskeletal organizationSpectrometryIdentified proteinsCo-isolatedDetection sensitivityProtein digestibility
2015
Exchange Protein Directly Activated by cAMP (EPAC) Regulates Neuronal Polarization through Rap1B
Muñoz-Llancao P, Henríquez D, Wilson C, Bodaleo F, Boddeke E, Lezoualc'h F, Schmidt M, González-Billault C. Exchange Protein Directly Activated by cAMP (EPAC) Regulates Neuronal Polarization through Rap1B. Journal Of Neuroscience 2015, 35: 11315-11329. PMID: 26269639, PMCID: PMC6605123, DOI: 10.1523/jneurosci.3645-14.2015.Peer-Reviewed Original ResearchConceptsNeuronal polarityAcquisition of neuronal polarityAxon specificationGenetic manipulationRap1b activationExchange proteinGuanine exchange factorKnock-outEpac signalingRegulate neuronal polarityProtein kinase A.Axon determinationRap GTPasesWild-type neuronsExchange factorActivation of protein kinase A.Polarity defectsMouse neuronsRat hippocampal neuronsMessenger cAMPMolecular eventsEpacMature axonsCAMP-dependent mechanismPharmacological inhibition
2012
The role of small GTPases in neuronal morphogenesis and polarity
Gonzalez‐Billault C, Muñoz‐Llancao P, Henriquez D, Wojnacki J, Conde C, Caceres A. The role of small GTPases in neuronal morphogenesis and polarity. Cytoskeleton 2012, 69: 464-485. PMID: 22605667, DOI: 10.1002/cm.21034.Peer-Reviewed Original ResearchConceptsNeuronal morphogenesisRegulation of cytoskeletal dynamicsParticipation of RhoASmall GTPasesActin cytoskeletonCytoskeletal dynamicsNeuronal polarityDynamic remodelingMorphogenetic eventsGTPaseCross-talkMorphogenesisAxonal elongationFamily membersSynaptic plasticityCdc42CytoskeletonRhoATC10ActinMicrotubulesRacRegulation
2009
Effect of the environment on the dendritic morphology of the rat auditory cortex
Bose M, Muñoz‐llancao P, Roychowdhury S, Nichols J, Jakkamsetti V, Porter B, Byrapureddy R, Salgado H, Kilgard M, Aboitiz F, Dagnino‐Subiabre A, Atzori M. Effect of the environment on the dendritic morphology of the rat auditory cortex. Synapse 2009, 64: 97-110. PMID: 19771593, PMCID: PMC2830877, DOI: 10.1002/syn.20710.Peer-Reviewed Original ResearchConceptsApical dendritic lengthBasal dendritic lengthApical dendritesDendritic lengthAuditory cortexSpine densityBasal dendritesShorter apical dendritesGolgi-Cox staining techniqueLayer-specific patternRat auditory cortexShort basal dendritesPrimary auditory cortexSensory deafnessInduce similar changesInfragranular neuronsLayer 2/3Neocortical plasticityExcitatory synapsesSholl analysisEnvironmental enrichmentStressed animalsGolgi-CoxStress-induced changesEnvironmental manipulationsChronic stress induces dendritic atrophy in the rat medial geniculate nucleus: Effects on auditory conditioning
Dagnino-Subiabre A, Muñoz-Llancao P, Terreros G, Wyneken U, Díaz-Véliz G, Porter B, Kilgard M, Atzori M, Aboitiz F. Chronic stress induces dendritic atrophy in the rat medial geniculate nucleus: Effects on auditory conditioning. Behavioural Brain Research 2009, 203: 88-96. PMID: 19397934, DOI: 10.1016/j.bbr.2009.04.024.Peer-Reviewed Original ResearchMeSH KeywordsAcoustic StimulationAnimalsAuditory PerceptionConditioning, ClassicalDendritesEscape ReactionFearFreezing Reaction, CatalepticGeniculate BodiesMaleNeuronsPhotic StimulationPosterior Thalamic NucleiRandom AllocationRatsRats, Sprague-DawleyReflex, StartleRestraint, PhysicalStress, PsychologicalVisual PerceptionConceptsMedial geniculate nucleusPosterior thalamic nucleusDendritic atrophyUnconditioned fearFear conditioningChronic stressStressed animalsAuditory conditioned stimulusAuditory fear conditioningAvoidance conditioned responseChronic restraint stressCued fear conditioningGeniculate nucleusSprague-Dawley ratsVisual fear conditioningFear conditioning protocolRestraint stressInferior colliculusThalamic nucleiConditioned stimulusConditioning trialsConditioned responseConditioning protocolUnconditioned responseAtrophy