2018
Male-Specific cAMP Signaling in the Hippocampus Controls Spatial Memory Deficits in a Mouse Model of Autism and Intellectual Disability
Zamarbide M, Mossa A, Muñoz-Llancao P, Wilkinson M, Pond H, Oaks A, Manzini M. Male-Specific cAMP Signaling in the Hippocampus Controls Spatial Memory Deficits in a Mouse Model of Autism and Intellectual Disability. Biological Psychiatry 2018, 85: 760-768. PMID: 30732858, PMCID: PMC6474812, DOI: 10.1016/j.biopsych.2018.12.013.Peer-Reviewed Original ResearchConceptsAutism spectrum disorderCognitive deficitsIntellectual disabilitySpectrum disorderNeurodevelopmental disordersMouse model of autismFemale miceRegulates cyclic adenosine monophosphateSpatial memory deficitsModel of autismPrevalence of neurodevelopmental disordersMale micePDE4D activityMale-specific mannerMemory deficitsSpatial memoryCyclic adenosine monophosphateIntracellular signalingBehavioral studiesMolecular deficitsAutismBehavioral differencesHippocampusCC2D1ADeficits
2017
Enhanced Peptide Detection Toward Single-Neuron Proteomics by Reversed-Phase Fractionation Capillary Electrophoresis Mass Spectrometry
Choi S, Lombard-Banek C, Muñoz-LLancao P, Manzini M, Nemes P. Enhanced Peptide Detection Toward Single-Neuron Proteomics by Reversed-Phase Fractionation Capillary Electrophoresis Mass Spectrometry. Journal Of The American Society For Mass Spectrometry 2017, 29: 913-922. PMID: 29147852, DOI: 10.1007/s13361-017-1838-1.Peer-Reviewed Original ResearchConceptsHigh-resolution mass spectrometryReversed-phaseCapillary electrophoresisMass spectrometryTandem high-resolution mass spectrometryCapillary electrophoresis mass spectrometryRates of peptide identificationsRP fractionationCE separationProtein groupsTrace-level sensitivityRP columnDetected peptidesPeptide identificationProducts of genesSpectral interferencesProtein identificationMS/MSTechnical triplicatesCytoskeletal organizationSpectrometryIdentified proteinsCo-isolatedDetection sensitivityProtein digestibility