2020
Simultaneous cortex-wide fluorescence Ca2+ imaging and whole-brain fMRI
Lake EMR, Ge X, Shen X, Herman P, Hyder F, Cardin JA, Higley MJ, Scheinost D, Papademetris X, Crair MC, Constable RT. Simultaneous cortex-wide fluorescence Ca2+ imaging and whole-brain fMRI. Nature Methods 2020, 17: 1262-1271. PMID: 33139894, PMCID: PMC7704940, DOI: 10.1038/s41592-020-00984-6.Peer-Reviewed Original ResearchConceptsOptical measurementsBrain functionTransgenic murine modelFunctional magnetic resonance imagingMagnetic resonance imagingFluorescent measuresWhole-brain fMRIMurine modelResonance imagingFluorescence Ca2Human brain functionConnectivity strengthBOLD signalBrain activityWidefieldLow frequencyImagingModalitiesTransfer functionMeasurementsCortex
2019
Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits
Barson D, Hamodi AS, Shen X, Lur G, Constable RT, Cardin JA, Crair MC, Higley MJ. Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits. Nature Methods 2019, 17: 107-113. PMID: 31686040, PMCID: PMC6946863, DOI: 10.1038/s41592-019-0625-2.Peer-Reviewed Original ResearchConceptsVasoactive intestinal peptide-expressing interneuronsBrain-wide network dynamicsTwo-photon calcium imagingEntire cortical mantleLocal neuronal circuitsLong-range cortical networksWidespread gene deliveryWidefield calciumPyramidal neuronsAwake miceCortical circuitsNeuronal activityCortical mantleTwo-photon imagingViral transduction methodsNeuronal circuitsCalcium imagingMouse brainFunctional connectivityLocal microcircuitsMammalian cortexCortical architectureCortical networksBrainActivity propagates
2008
Cortical Adenylyl Cyclase 1 Is Required for Thalamocortical Synapse Maturation and Aspects of Layer IV Barrel Development
Iwasato T, Inan M, Kanki H, Erzurumlu RS, Itohara S, Crair MC. Cortical Adenylyl Cyclase 1 Is Required for Thalamocortical Synapse Maturation and Aspects of Layer IV Barrel Development. Journal Of Neuroscience 2008, 28: 5931-5943. PMID: 18524897, PMCID: PMC2733830, DOI: 10.1523/jneurosci.0815-08.2008.Peer-Reviewed Original ResearchConceptsTC synapsesLayer IV barrel neuronsCritical period plasticityPrimary somatosensory cortexFormation of barrelsAdenylyl cyclase 1Knock-out (KO) miceType 1 adenylyl cyclaseDendritic asymmetryBarrel neuronsThalamocortical synapsesActivity-dependent mannerTrigeminal pathwayFormation of cAMPSomatosensory cortexBarrel cortexBarrel developmentSynapse maturationPresynaptic maturationBarrel hollowsTC axonsSubcortical regionsFunctional maturationMutant miceBRL mice
2007
Increased Thalamocortical Synaptic Response and Decreased Layer IV Innervation in GAP-43 Knockout Mice
Albright MJ, Weston MC, Inan M, Rosenmund C, Crair MC. Increased Thalamocortical Synaptic Response and Decreased Layer IV Innervation in GAP-43 Knockout Mice. Journal Of Neurophysiology 2007, 98: 1610-1625. PMID: 17581849, DOI: 10.1152/jn.00219.2007.Peer-Reviewed Original ResearchConceptsExcitatory postsynaptic potentialsField excitatory postsynaptic potentialsGAP-43Thalamocortical synapsesSynaptic responsesCompetitive glutamate receptor antagonistN-methyl-D-aspartate receptorsAcute brain slice preparationBarrel map formationThalamocortical synaptic responsesWild-type littermate controlsGlutamate receptor antagonistsBrain slice preparationGrowth-associated proteinThalamic innervationThalamic neuronsBarrel mapReceptor antagonistIsoxazolepropionate (AMPA) receptorsPostsynaptic potentialsLayer IVSlice preparationBarrel cortexSynaptic transmissionAMPAR function
2006
Barrel Map Development Relies on Protein Kinase A Regulatory Subunit IIβ-Mediated cAMP Signaling
Inan M, Lu HC, Albright MJ, She WC, Crair MC. Barrel Map Development Relies on Protein Kinase A Regulatory Subunit IIβ-Mediated cAMP Signaling. Journal Of Neuroscience 2006, 26: 4338-4349. PMID: 16624954, PMCID: PMC6674004, DOI: 10.1523/jneurosci.3745-05.2006.Peer-Reviewed Original ResearchConceptsBarrel map formationLayer IV neuronsActivity-dependent developmentAMPA receptor functionCAMP/PKA-dependent pathwayLong-term potentiationThalamocortical synapsesThalamocortical afferentsTC synapsesThalamocortical synapseBarrel cortexPKA targetsBarrel patternCortical developmentPKA-dependent pathwayBrain circuitryPostsynaptic processesSynapse formationReceptor functionCAMP-dependent protein kinaseHebbian mechanismsDevelopmental increaseMiceSynapsesActivity-dependent models
2003
Adenylyl cyclase I regulates AMPA receptor trafficking during mouse cortical 'barrel' map development
Lu HC, She WC, Plas DT, Neumann PE, Janz R, Crair MC. Adenylyl cyclase I regulates AMPA receptor trafficking during mouse cortical 'barrel' map development. Nature Neuroscience 2003, 6: 939-947. PMID: 12897788, DOI: 10.1038/nn1106.Peer-Reviewed Original ResearchConceptsLong-term depressionLong-term potentiationAMPA receptor traffickingThalamocortical synapsesBarrelless miceBarrel map formationSynaptic AMPAR traffickingAMPAR subunit GluR1Activity-dependent mechanismsReceptor traffickingAC1 activityFunctional AMPARsSurface GluR1Thalamocortical afferentsMap formationAdenylyl cyclase IBarrel mapSubunit GluR1Cortical map formationAMPAR traffickingProtein kinase A (PKA) activitySynapsesAdenylyl cyclaseMiceImmature state
1999
The Nuclear Orphan Receptor COUP-TFI Is Required for Differentiation of Subplate Neurons and Guidance of Thalamocortical Axons
Zhou C, Qiu Y, Pereira F, Crair M, Tsai S, Tsai M. The Nuclear Orphan Receptor COUP-TFI Is Required for Differentiation of Subplate Neurons and Guidance of Thalamocortical Axons. Neuron 1999, 24: 847-859. PMID: 10624948, DOI: 10.1016/s0896-6273(00)81032-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntimetabolitesAxonsBromodeoxyuridineCarbocyaninesCell DeathCell DifferentiationCerebral CortexCOUP Transcription Factor IDNA-Binding ProteinsFluorescent DyesImmunohistochemistryIn Situ HybridizationMaleMiceMutationNeural PathwaysNeuronsReceptors, GlucocorticoidThalamusTranscription FactorsConceptsSubplate neuronsThalamocortical projectionsCortical layer IVLayer IV neuronsCell deathCorticothalamic connectivityAfferent innervationCerebral cortexThalamocortical axonsLayer IVNervous systemExcessive cell deathFactor INeuronal developmentNeuronsNuclear receptorsPremature cell deathInnervationImproper differentiationImportant regulatorOrphan memberDeathCritical roleDifferentiationFailure