Featured Publications
Macrophage migration inhibitory factor deficiency in chronic obstructive pulmonary disease
Sauler M, Leng L, Trentalange M, Haslip M, Shan P, Piecychna M, Zhang Y, Andrews N, Mannam P, Allore H, Fried T, Bucala R, Lee PJ. Macrophage migration inhibitory factor deficiency in chronic obstructive pulmonary disease. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2014, 306: l487-l496. PMID: 24441872, PMCID: PMC3949087, DOI: 10.1152/ajplung.00284.2013.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAge FactorsAgedAged, 80 and overAnimalsApoptosisCellular SenescenceCyclin-Dependent Kinase Inhibitor p16Cyclin-Dependent Kinase Inhibitor p21EmphysemaFemaleHumansIntramolecular OxidoreductasesLungMacrophage Migration-Inhibitory FactorsMaleMiceMice, Inbred C57BLMice, KnockoutMiddle AgedPulmonary Disease, Chronic ObstructiveReceptors, ImmunologicSmokeSmokingTobaccoTumor Suppressor Protein p53Young AdultConceptsChronic obstructive pulmonary diseaseMacrophage migration inhibitory factorPathogenesis of COPDDevelopment of COPDMIF receptor CD74Obstructive pulmonary diseasePulmonary diseaseWT miceReceptor CD74Role of MIFMacrophage migration inhibitory factor deficiencyMigration inhibitory factorNormal alveolar structureMo of ageWild-type controlsMIF concentrationsFormer smokersLung volumePlasma concentrationsSpontaneous emphysemaFactor deficiencyCigarette smokePleiotropic cytokineInhibitory factorAlveolar structuresEndothelial CD74 mediates macrophage migration inhibitory factor protection in hyperoxic lung injury
Sauler M, Zhang Y, Min J, Leng L, Shan P, Roberts S, Jorgensen WL, Bucala R, Lee PJ. Endothelial CD74 mediates macrophage migration inhibitory factor protection in hyperoxic lung injury. The FASEB Journal 2015, 29: 1940-1949. PMID: 25609432, PMCID: PMC4415022, DOI: 10.1096/fj.14-260299.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorLung endothelial cellsMurine lung endothelial cellsLung injuryEndothelial cellsProtective effectExogenous MIFCD74 deficient miceCD74-dependent mannerHours of hyperoxiaMIF receptor CD74Acute lung injuryHyperoxic lung injuryBronchoalveolar lavage proteinMigration inhibitory factorBronchoalveolar proteinMIF deficiencyMedian survivalEndothelial injuryWT miceAcute oxidative stressReceptor CD74Lavage proteinReceptor antagonistReceptor inhibitors
2019
Endothelial toll‐like receptor 4 maintains lung integrity via epigenetic suppression of p16INK4a
Kim S, Shan P, Hwangbo C, Zhang Y, Min J, Zhang X, Ardito T, Li A, Peng T, Sauler M, Lee PJ. Endothelial toll‐like receptor 4 maintains lung integrity via epigenetic suppression of p16INK4a. Aging Cell 2019, 18: e12914. PMID: 30790400, PMCID: PMC6516428, DOI: 10.1111/acel.12914.Peer-Reviewed Original ResearchConceptsToll-like receptor 4Senescence-related gene expressionLung integritySenescence-associated genesReceptor 4New functional roleLung ECsInnate immune receptor toll-like receptor 4Immune receptor toll-like receptor 4Role of TLR4Endothelial Toll-like receptor 4Receptor Toll-like receptor 4Epigenetic regulationHistone H4Epigenetic suppressionTLR4-/- miceGene expressionEndogenous p16Molecular mechanismsWild-type miceNovel roleExpression of p16Lung endothelial cellsFunctional roleDistinct roles
2018
Endothelial cell‐secreted MIF reduces pericyte contractility and enhances neutrophil extravasation
Pellowe AS, Sauler M, Hou Y, Merola J, Liu R, Calderon B, Lauridsen HM, Harris MR, Leng L, Zhang Y, Tilstam PV, Pober JS, Bucala R, Lee PJ, Gonzalez AL. Endothelial cell‐secreted MIF reduces pericyte contractility and enhances neutrophil extravasation. The FASEB Journal 2018, 33: 2171-2186. PMID: 30252532, PMCID: PMC6338650, DOI: 10.1096/fj.201800480r.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorNeutrophil transmigrationNeutrophil extravasationPericyte contractilitySelective deletionMIF knockout miceAcute lung injuryMigration inhibitory factorHuman neutrophil transmigrationMyosin light chain phosphorylationLung injuryNeutrophil infiltrationBronchoalveolar lavageInflammatory disordersKnockout miceInhibitory factorMicro-ELISAContractilityLight chain phosphorylationPhosphorylated myosin light chainEndothelial cellsBarrier functionExtravasationPericytesParacrine signals
2014
Endothelial PINK1 Mediates the Protective Effects of NLRP3 Deficiency during Lethal Oxidant Injury
Zhang Y, Sauler M, Shinn AS, Gong H, Haslip M, Shan P, Mannam P, Lee PJ. Endothelial PINK1 Mediates the Protective Effects of NLRP3 Deficiency during Lethal Oxidant Injury. The Journal Of Immunology 2014, 192: 5296-5304. PMID: 24778451, PMCID: PMC4047670, DOI: 10.4049/jimmunol.1400653.Peer-Reviewed Original ResearchConceptsAcute respiratory failurePTEN-induced putative kinase 1Respiratory failureWild-type miceNLRP3 deficiencyAutophagy/mitophagySpecific therapyProtective effectProtein NLRP3Oxidant injuryLung endotheliumHigh mortalityHyperoxiaGenetic deletionHigher basalIntermediate susceptibilityMiceOxidant generationPutative kinase 1PINK1 expressionProteasome activationNovel roleDouble knockoutEndotheliumKinase 1