2020
Adaptive tuning of cell sensory diversity without changes in gene expression
Kamino K, Keegstra JM, Long J, Emonet T, Shimizu TS. Adaptive tuning of cell sensory diversity without changes in gene expression. Science Advances 2020, 6: eabc1087. PMID: 33188019, PMCID: PMC7673753, DOI: 10.1126/sciadv.abc1087.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalBacterial ProteinsChemotaxisEscherichia coliEscherichia coli ProteinsGene ExpressionConceptsPhenotypic diversityGene expressionCell variationDiversity of phenotypesChemoreceptor TarPosttranslational modificationsAllosteric couplingEnvironmental cuesChemotaxis networkCovalent modificationEnvironmental changesDiversitySensory diversityCell populationsCellsExpressionSuch betsPhenotypeEscherichiaPopulationPrevious studiesModificationVariationSignalsChemoreceptors
2011
High‐throughput, subpixel precision analysis of bacterial morphogenesis and intracellular spatio‐temporal dynamics
Sliusarenko O, Heinritz J, Emonet T, Jacobs‐Wagner C. High‐throughput, subpixel precision analysis of bacterial morphogenesis and intracellular spatio‐temporal dynamics. Molecular Microbiology 2011, 80: 612-627. PMID: 21414037, PMCID: PMC3090749, DOI: 10.1111/j.1365-2958.2011.07579.x.Peer-Reviewed Original ResearchConceptsCell cycle regulationCell lineage trackingComplex spatial organizationBacterial morphogenesisCaulobacter crescentusCellular processesCycle regulationLineage trackingChromosomal originFilamentous cellsSpatio-temporal dynamicsIntracellular componentsExternal cuesCell lengthMorphogenesisSpatial organizationSeparation eventsCellsUnbiased studyNovel aspectsCrescentusSegment cellsProtein concentrationCrowded imagesSilencing
2010
Fine-Tuning of Chemotactic Response in E. coli Determined by High-Throughput Capillary Assay
Park H, Guet CC, Emonet T, Cluzel P. Fine-Tuning of Chemotactic Response in E. coli Determined by High-Throughput Capillary Assay. Current Microbiology 2010, 62: 764-769. PMID: 20972792, PMCID: PMC3230253, DOI: 10.1007/s00284-010-9778-z.Peer-Reviewed Original ResearchMeSH KeywordsAspartic AcidBacterial ProteinsChemotaxisEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialGenes, ReporterMethyltransferasesConceptsChemotactic responseWild-type levelsCapillary assayRobust chemotactic responseE. coliWild-type behaviorE. coli cellsPerfect adaptationDepletion of nutrientsColi cellsFusion proteinCellular growthChemotactic behaviorProteinIntracellular concentrationChemotactic proteinColiAssaysChemotaxisAdaptationSwimming bacteriaCheB.Effects of growthCheRGrowth
2006
Dynamical Determinants of Drug-Inducible Gene Expression in a Single Bacterium
Le TT, Emonet T, Harlepp S, Guet C, Cluzel P. Dynamical Determinants of Drug-Inducible Gene Expression in a Single Bacterium. Biophysical Journal 2006, 90: 3315-3321. PMID: 16461398, PMCID: PMC1432126, DOI: 10.1529/biophysj.105.073353.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsCapsid ProteinsDrug Resistance, Multiple, BacterialEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialLevivirusLipoproteinsMembrane Transport ProteinsMultidrug Resistance-Associated ProteinsPromoter Regions, GeneticRNA, BacterialRNA, MessengerTetracyclineConceptsGene expressionDrug-inducible gene expressionAcrAB-TolC multidrug effluxTet promoterMultidrug effluxEfflux systemDrug-inducible systemSingle-cell experimentsTranscriptional pulsesSingle bacteriumMultidrug efflux systemsTranscriptional responseEnvironmental signalsGenetic systemRNA degradationFluorescence correlation spectroscopyCellular RNATranscription activityRate of synthesisPromoterInducer concentrationTranscriptionRNA levelsBacteriumPrimitive examples
2005
Real-time RNA profiling within a single bacterium
Le TT, Harlepp S, Guet C, Dittmar K, Emonet T, Pan T, Cluzel P. Real-time RNA profiling within a single bacterium. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 9160-9164. PMID: 15967986, PMCID: PMC1166617, DOI: 10.1073/pnas.0503311102.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCell CycleCell ProliferationDose-Response Relationship, DrugDrug Resistance, MultipleEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialGenes, ReporterGenotypeGreen Fluorescent ProteinsModels, StatisticalPlasmidsPromoter Regions, GeneticProtein BindingRecombinant Fusion ProteinsRNARNA, MessengerSpectrometry, FluorescenceSpliceosomesTime FactorsTranscription, GeneticConceptsRNA profilingTranscriptional dynamicsInducible gene expression systemGene expression systemSingle cellsSpecific RNA levelsSingle bacteriumRNA levelsGenetic systemSpecific promotersFluorescence correlation spectroscopyExpression systemEscherichia coliEfflux pump systemGenetic deletionProfilingBacteriumPopulation measurementsKey determinantAntibiotic resistanceProkaryotesCellsPromoterCorrelation spectroscopyRNA