2011
High‐throughput, subpixel precision analysis of bacterial morphogenesis and intracellular spatio‐temporal dynamics
Sliusarenko O, Heinritz J, Emonet T, Jacobs‐Wagner C. High‐throughput, subpixel precision analysis of bacterial morphogenesis and intracellular spatio‐temporal dynamics. Molecular Microbiology 2011, 80: 612-627. PMID: 21414037, PMCID: PMC3090749, DOI: 10.1111/j.1365-2958.2011.07579.x.Peer-Reviewed Original ResearchConceptsCell cycle regulationCell lineage trackingComplex spatial organizationBacterial morphogenesisCaulobacter crescentusCellular processesCycle regulationLineage trackingChromosomal originFilamentous cellsSpatio-temporal dynamicsIntracellular componentsExternal cuesCell lengthMorphogenesisSpatial organizationSeparation eventsCellsUnbiased studyNovel aspectsCrescentusSegment cellsProtein concentrationCrowded imagesSilencing
2010
Spatial organization of the flow of genetic information in bacteria
Montero Llopis P, Jackson AF, Sliusarenko O, Surovtsev I, Heinritz J, Emonet T, Jacobs-Wagner C. Spatial organization of the flow of genetic information in bacteria. Nature 2010, 466: 77-81. PMID: 20562858, PMCID: PMC2896451, DOI: 10.1038/nature09152.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCaulobacter crescentusChaperoninsChromosomes, BacterialDiffusionDNA, BacterialEndoribonucleasesEscherichia coliGene Expression Regulation, BacterialIn Situ Hybridization, FluorescenceLac OperonProtein BiosynthesisRibosomesRNA StabilityRNA TransportRNA, BacterialRNA, MessengerTranscription, GeneticConceptsSites of transcriptionC. crescentusCaulobacter crescentusEukaryotic cellsCellular physiologyMRNA decayMature mRNAMRNA processesRNase EMRNA substratesMRNA localizationGenetic informationGene expressionBacterial cellsEscherichia coliQuantitative fluorescenceCrescentusSitu hybridizationSpatial organizationMRNABacteriaLimited dispersionCellsTranscriptionTranslationProcessivity of peptidoglycan synthesis provides a built-in mechanism for the robustness of straight-rod cell morphology
Sliusarenko O, Cabeen MT, Wolgemuth CW, Jacobs-Wagner C, Emonet T. Processivity of peptidoglycan synthesis provides a built-in mechanism for the robustness of straight-rod cell morphology. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 10086-10091. PMID: 20479277, PMCID: PMC2890421, DOI: 10.1073/pnas.1000737107.Peer-Reviewed Original Research
2009
RodZ, a component of the bacterial core morphogenic apparatus
Alyahya SA, Alexander R, Costa T, Henriques AO, Emonet T, Jacobs-Wagner C. RodZ, a component of the bacterial core morphogenic apparatus. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 1239-1244. PMID: 19164570, PMCID: PMC2633561, DOI: 10.1073/pnas.0810794106.Peer-Reviewed Original ResearchConceptsCell morphogenesisExtracellular C-terminal domainBacterial cell morphogenesisConservation of functionTerminal cytoplasmic domainC-terminal domainN-terminal domainMultiple sequence alignmentMreB cytoskeletonBacterial kingdomAncient functionCaulobacter crescentusGrowth machineryLocalization determinantsHelix motifRodZTransmembrane sequenceBacterial phylaCytoplasmic domainGenomic analysisTerminal domainMolecular basisPeptidoglycan synthesisCellular formCell cycle