2021
A Primed Subpopulation of Bacteria Enables Rapid Expression of the Type 3 Secretion System in Pseudomonas aeruginosa
Lin CK, Lee DSW, McKeithen-Mead S, Emonet T, Kazmierczak B. A Primed Subpopulation of Bacteria Enables Rapid Expression of the Type 3 Secretion System in Pseudomonas aeruginosa. MBio 2021, 12: 10.1128/mbio.00831-21. PMID: 34154400, PMCID: PMC8262847, DOI: 10.1128/mbio.00831-21.Peer-Reviewed Original ResearchConceptsType 3 secretion systemSecretion systemT3SS expressionVirulence traitsSpecific virulence traitsHuman disease severityComplex nanomachinesT3SS genesP. aeruginosa cellsReproductive fitnessIsogenic cellsHeterogeneous expressionCell envelopeT3SS effectorsMotility organellesReservoir of cellsCritical virulence traitsGene expressionRegulatory mechanismsSubpopulation of cellsGram-negative pathogensFluorescent reportersDivision timeP. aeruginosaPseudomonas aeruginosa
2006
Dynamical Determinants of Drug-Inducible Gene Expression in a Single Bacterium
Le TT, Emonet T, Harlepp S, Guet C, Cluzel P. Dynamical Determinants of Drug-Inducible Gene Expression in a Single Bacterium. Biophysical Journal 2006, 90: 3315-3321. PMID: 16461398, PMCID: PMC1432126, DOI: 10.1529/biophysj.105.073353.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsCapsid ProteinsDrug Resistance, Multiple, BacterialEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialLevivirusLipoproteinsMembrane Transport ProteinsMultidrug Resistance-Associated ProteinsPromoter Regions, GeneticRNA, BacterialRNA, MessengerTetracyclineConceptsGene expressionDrug-inducible gene expressionAcrAB-TolC multidrug effluxTet promoterMultidrug effluxEfflux systemDrug-inducible systemSingle-cell experimentsTranscriptional pulsesSingle bacteriumMultidrug efflux systemsTranscriptional responseEnvironmental signalsGenetic systemRNA degradationFluorescence correlation spectroscopyCellular RNATranscription activityRate of synthesisPromoterInducer concentrationTranscriptionRNA levelsBacteriumPrimitive examples
2005
Real-time RNA profiling within a single bacterium
Le TT, Harlepp S, Guet C, Dittmar K, Emonet T, Pan T, Cluzel P. Real-time RNA profiling within a single bacterium. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 9160-9164. PMID: 15967986, PMCID: PMC1166617, DOI: 10.1073/pnas.0503311102.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCell CycleCell ProliferationDose-Response Relationship, DrugDrug Resistance, MultipleEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialGenes, ReporterGenotypeGreen Fluorescent ProteinsModels, StatisticalPlasmidsPromoter Regions, GeneticProtein BindingRecombinant Fusion ProteinsRNARNA, MessengerSpectrometry, FluorescenceSpliceosomesTime FactorsTranscription, GeneticConceptsRNA profilingTranscriptional dynamicsInducible gene expression systemGene expression systemSingle cellsSpecific RNA levelsSingle bacteriumRNA levelsGenetic systemSpecific promotersFluorescence correlation spectroscopyExpression systemEscherichia coliEfflux pump systemGenetic deletionProfilingBacteriumPopulation measurementsKey determinantAntibiotic resistanceProkaryotesCellsPromoterCorrelation spectroscopyRNA