2006
Dynamical Determinants of Drug-Inducible Gene Expression in a Single Bacterium
Le TT, Emonet T, Harlepp S, Guet C, Cluzel P. Dynamical Determinants of Drug-Inducible Gene Expression in a Single Bacterium. Biophysical Journal 2006, 90: 3315-3321. PMID: 16461398, PMCID: PMC1432126, DOI: 10.1529/biophysj.105.073353.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsCapsid ProteinsDrug Resistance, Multiple, BacterialEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialLevivirusLipoproteinsMembrane Transport ProteinsMultidrug Resistance-Associated ProteinsPromoter Regions, GeneticRNA, BacterialRNA, MessengerTetracyclineConceptsGene expressionDrug-inducible gene expressionAcrAB-TolC multidrug effluxTet promoterMultidrug effluxEfflux systemDrug-inducible systemSingle-cell experimentsTranscriptional pulsesSingle bacteriumMultidrug efflux systemsTranscriptional responseEnvironmental signalsGenetic systemRNA degradationFluorescence correlation spectroscopyCellular RNATranscription activityRate of synthesisPromoterInducer concentrationTranscriptionRNA levelsBacteriumPrimitive examples
2005
Real-time RNA profiling within a single bacterium
Le TT, Harlepp S, Guet C, Dittmar K, Emonet T, Pan T, Cluzel P. Real-time RNA profiling within a single bacterium. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 9160-9164. PMID: 15967986, PMCID: PMC1166617, DOI: 10.1073/pnas.0503311102.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCell CycleCell ProliferationDose-Response Relationship, DrugDrug Resistance, MultipleEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialGenes, ReporterGenotypeGreen Fluorescent ProteinsModels, StatisticalPlasmidsPromoter Regions, GeneticProtein BindingRecombinant Fusion ProteinsRNARNA, MessengerSpectrometry, FluorescenceSpliceosomesTime FactorsTranscription, GeneticConceptsRNA profilingTranscriptional dynamicsInducible gene expression systemGene expression systemSingle cellsSpecific RNA levelsSingle bacteriumRNA levelsGenetic systemSpecific promotersFluorescence correlation spectroscopyExpression systemEscherichia coliEfflux pump systemGenetic deletionProfilingBacteriumPopulation measurementsKey determinantAntibiotic resistanceProkaryotesCellsPromoterCorrelation spectroscopyRNAAgentCell: a digital single-cell assay for bacterial chemotaxis
Emonet T, Macal CM, North MJ, Wickersham CE, Cluzel P. AgentCell: a digital single-cell assay for bacterial chemotaxis. Bioinformatics 2005, 21: 2714-2721. PMID: 15774553, DOI: 10.1093/bioinformatics/bti391.Peer-Reviewed Original ResearchConceptsBacterial chemotaxisSingle-cell biologySingle-cell assaysSingle-cell levelChemotaxis networkCellular behaviorBacterial populationsIntracellular processesIndividual cellsSingle cellsSwimming cellsMolecular interactionsChemotaxis assaysBiological systemsNew computational approachCellsComputational approachChemotaxisAssaysFlagellaBiologyBacterium
2004
From molecular noise to behavioural variability in a single bacterium
Korobkova E, Emonet T, Vilar JM, Shimizu TS, Cluzel P. From molecular noise to behavioural variability in a single bacterium. Nature 2004, 428: 574-578. PMID: 15058306, DOI: 10.1038/nature02404.Peer-Reviewed Original ResearchConceptsChemotaxis networkSingle-cell levelSignal transductionPopulation measurementsIntracellular networksMolecular noiseBehavioral variationMolecular eventsBiological complexityIndividual bacteriaNon-stimulated cellsEscherichia coliBehavioral variabilitySingle bacteriumTemporal fluctuationsStatistical fluctuationsTransductionSuch variabilityTemporal variationBacteriumColiGeneral understandingCertain propertiesBacteriaPathway