2022
Stem cell-derived sensory neurons modelling inherited erythromelalgia: normalization of excitability
Alsaloum M, Labau JIR, Liu S, Effraim P, Waxman SG. Stem cell-derived sensory neurons modelling inherited erythromelalgia: normalization of excitability. Brain 2022, 146: 359-371. PMID: 35088838, PMCID: PMC10060693, DOI: 10.1093/brain/awac031.Peer-Reviewed Original ResearchConceptsSensory neuronsPluripotent stem cell-derived sensory neuronsDynamic clamp electrophysiologyMediators of painUnmet healthcare needsEffective therapeutic approachErythromelalgia mutationAmeliorate painNeuronal hyperexcitabilityPain disordersClinical studiesNeuronal excitabilityPreclinical studiesTherapeutic approachesEffective treatmentNaV1.7 currentsBaseline levelsClamp electrophysiologyHealthcare needsNav1.7 channelsPainErythromelalgiaHyperexcitabilityFunction mutationsNav1.7
2021
Contributions of NaV1.8 and NaV1.9 to excitability in human induced pluripotent stem-cell derived somatosensory neurons
Alsaloum M, Labau JIR, Liu S, Estacion M, Zhao P, Dib-Hajj F, Waxman SG. Contributions of NaV1.8 and NaV1.9 to excitability in human induced pluripotent stem-cell derived somatosensory neurons. Scientific Reports 2021, 11: 24283. PMID: 34930944, PMCID: PMC8688473, DOI: 10.1038/s41598-021-03608-x.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAutopsyCell DifferentiationElectrophysiologyHumansImmunohistochemistryInduced Pluripotent Stem CellsMembrane PotentialsMutationNAV1.8 Voltage-Gated Sodium ChannelNAV1.9 Voltage-Gated Sodium ChannelNeuronsNeurosciencesPainPatch-Clamp TechniquesProtein IsoformsSensory Receptor CellsSomatosensory CortexConceptsNeuronal excitabilitySomatosensory neuronsPluripotent stem cell-derived sensory neuronsDynamic clamp electrophysiologyTreatment of painPromising novel modalityVoltage-gated sodium channelsSodium channel isoformsNeuronal membrane potentialGenetic knockout modelsNav1.9 currentsPharmacologic blockSensory neuronsNav1.8Cellular correlatesRepetitive firingClamp electrophysiologyExcitabilityNeuronal backgroundNovel modalityChannel isoformsSodium channelsNeuronsNav1.9Knockout modelsiPSCs and DRGs: stepping stones to new pain therapies
Alsaloum M, Waxman SG. iPSCs and DRGs: stepping stones to new pain therapies. Trends In Molecular Medicine 2021, 28: 110-122. PMID: 34933815, PMCID: PMC8810720, DOI: 10.1016/j.molmed.2021.11.005.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHuman DRG neuronsDRG neuronsDorsal root ganglion neuronsTreatment of painNovel pain therapeuticsNew pain therapiesVoltage-gated sodium channelsPain therapyPain therapeuticsGanglion neuronsTreatment optionsPain signalsSpinal cordPreclinical resultsPreclinical platformSensory neuronsPainNeuronsSodium channelsClinical translationIPSCsBlockadeCordTherapyExcitability
2020
Status of peripheral sodium channel blockers for non-addictive pain treatment
Alsaloum M, Higerd GP, Effraim PR, Waxman SG. Status of peripheral sodium channel blockers for non-addictive pain treatment. Nature Reviews Neurology 2020, 16: 689-705. PMID: 33110213, DOI: 10.1038/s41582-020-00415-2.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsPain conditionsPain treatmentDose-limiting adverse effectUnmet health care needsAdverse effectsMediators of painMultiple pain conditionsCommon pain conditionsCardiac adverse effectsTrigeminal ganglion neuronsTreatment of painDorsal root gangliaPeripheral nervous systemHuman pain disordersSodium channel blockersHealth care needsVoltage-gated sodium channelsImproved therapeutic agentsCurrent medicationsPain disordersPain managementGanglion neuronsRoot gangliaSafe treatmentClinical trials