2001
Changes in the phosphorylation of initiation factor eIF‐2α, elongation factor eEF‐2 and p70 S6 kinase after transient focal cerebral ischaemia in mice
Althausen S, Mengesdorf T, Mies G, Oláh L, Nairn A, Proud C, Paschen W. Changes in the phosphorylation of initiation factor eIF‐2α, elongation factor eEF‐2 and p70 S6 kinase after transient focal cerebral ischaemia in mice. Journal Of Neurochemistry 2001, 78: 779-787. PMID: 11520898, DOI: 10.1046/j.1471-4159.2001.00462.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCerebral CortexCerebrovascular CirculationEnzyme InhibitorsEukaryotic Initiation Factor-2ImmunoblottingImmunohistochemistryIschemic Attack, TransientLaser-Doppler FlowmetryMiceMiddle Cerebral ArteryNeuronsPeptide Elongation Factor 2PhosphorylationProtein BiosynthesisRatsRats, WistarRibosomal Protein S6 KinasesThapsigarginConceptsIschaemia-induced changesTransient focal cerebral ischaemiaMiddle cerebral arteryFocal cerebral ischaemiaCerebral ischaemiaP70 S6 kinaseLeft middle cerebral arteryControl levelsTransient cerebral ischaemiaTransient MCA occlusionNeuronal cell injuryPrimary neuronal cell culturesTransient focal ischaemiaElongation factor eEF-2Endoplasmic reticulum calcium pumpEIF-2alpha phosphorylationER calcium homeostasisNeuronal cell culturesS6 kinaseProtein synthesisWestern blot analysisMCA occlusionMCA territoryMin occlusionCerebral artery
1999
Zinc Inhibits Protein Synthesis in Neurons POTENTIAL ROLE OF PHOSPHORYLATION OF TRANSLATION INITIATION FACTOR-2α*
Alirezaei M, Nairn A, Glowinski J, Prémont J, Marin P. Zinc Inhibits Protein Synthesis in Neurons POTENTIAL ROLE OF PHOSPHORYLATION OF TRANSLATION INITIATION FACTOR-2α*. Journal Of Biological Chemistry 1999, 274: 32433-32438. PMID: 10542287, DOI: 10.1074/jbc.274.45.32433.Peer-Reviewed Original ResearchConceptsCultured cortical neuronsEukaryotic elongation factor 2Central nervous systemFactor 2Translation initiation factor 2αProtein synthesisCerebral cortexCortical neuronsNervous systemProfound inhibitionSustained increaseBasal levelsTransient increaseBasal ratePotential roleProgressive decreaseInhibits protein synthesisNeuronsAmount of polyribosomesElongation factor 2EIF-2alpha phosphorylationAlpha subunitInhibitionPhosphorylationEukaryotic initiation factor 2