2023
Microcephaly-associated protein WDR62 shuttles from the Golgi apparatus to the spindle poles in human neural progenitors
Dell'Amico C, Salavarria M, Takeo Y, Saotome I, Dell'Anno M, Galimberti M, Pellegrino E, Cattaneo E, Louvi A, Onorati M. Microcephaly-associated protein WDR62 shuttles from the Golgi apparatus to the spindle poles in human neural progenitors. ELife 2023, 12: e81716. PMID: 37272619, PMCID: PMC10241521, DOI: 10.7554/elife.81716.Peer-Reviewed Original ResearchConceptsHuman fetal brain tissueStructural brain abnormalitiesC-terminal truncating mutationsFetal brain tissueEtiology of microcephalySevere neurodevelopmental abnormalitiesStem cellsNeuroepithelial stem cellsHuman neural progenitorsHuman brain developmentBrain abnormalitiesCommon causeNeurodevelopmental abnormalitiesAutosomal recessive primary microcephalyBrain tissueBrain developmentCerebral organoidsMicrocephalyUnaffected parentsTruncating mutationsNeural progenitorsHuman neurodevelopmentAbnormalitiesPleiotropic functionsCritical hub
2019
Id4 Downstream of Notch2 Maintains Neural Stem Cell Quiescence in the Adult Hippocampus
Zhang R, Boareto M, Engler A, Louvi A, Giachino C, Iber D, Taylor V. Id4 Downstream of Notch2 Maintains Neural Stem Cell Quiescence in the Adult Hippocampus. Cell Reports 2019, 28: 1485-1498.e6. PMID: 31390563, DOI: 10.1016/j.celrep.2019.07.014.Peer-Reviewed Original ResearchConceptsNeural stem cellsDentate gyrusNSC quiescenceAdult mouse hippocampal dentate gyrusNSC proliferationMouse hippocampal dentate gyrusAdult dentate gyrusHippocampal dentate gyrusExpense of neurogenesisNeural stem cell quiescenceId4 knockdownAdult hippocampusNeuron generationId4 expressionNeuronal differentiationCell cycle entryNSC activationMajor effectorStem cell quiescenceNotch2NeurogenesisCell quiescenceStem cellsDownstream targetsNSC maintenance
2018
Notch2 Signaling Maintains NSC Quiescence in the Murine Ventricular-Subventricular Zone
Engler A, Rolando C, Giachino C, Saotome I, Erni A, Brien C, Zhang R, Zimber-Strobl U, Radtke F, Artavanis-Tsakonas S, Louvi A, Taylor V. Notch2 Signaling Maintains NSC Quiescence in the Murine Ventricular-Subventricular Zone. Cell Reports 2018, 22: 992-1002. PMID: 29386140, DOI: 10.1016/j.celrep.2017.12.094.Peer-Reviewed Original ResearchConceptsV-SVZ neural stem cellsVentricular-subventricular zoneNeural stem cellsQuiescent neural stem cellsRostral migratory streamNew olfactory bulb neuronsNSC quiescenceOlfactory bulb neuronsLoss of Notch2Bulb neuronsNew neuronsAdult forebrainOB lineageAging-like phenotypesMigratory streamNotch2 functionNeuronsNotch2Canonical Notch signalingNeurogenesisStem cellsNotch signalingCell cycleForebrainQuiescence
2016
Notch1 and Notch2 receptors regulate mouse and human gastric antral epithelial cell homoeostasis
Gifford GB, Demitrack ES, Keeley TM, Tam A, La Cunza N, Dedhia PH, Spence JR, Simeone DM, Saotome I, Louvi A, Siebel CW, Samuelson LC. Notch1 and Notch2 receptors regulate mouse and human gastric antral epithelial cell homoeostasis. Gut 2016, 66: 1001. PMID: 26933171, PMCID: PMC5009003, DOI: 10.1136/gutjnl-2015-310811.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, Monoclonal, HumanizedApoptosisCell DifferentiationCell ProliferationCells, CulturedDibenzazepinesEpithelial CellsFemaleGastric MucosaGene ExpressionHomeostasisHumansMaleMiceMice, Inbred C57BLMice, TransgenicOrganoidsPyloric AntrumReceptor, Notch1Receptor, Notch2Receptors, G-Protein-CoupledSignal TransductionStem CellsConceptsEpithelial cell homeostasisCell homeostasisNotch receptorsNotch inhibitor dibenzazepineGlobal Notch inhibitionStem cellsAntral stem cellsHuman antral glandsAnalysis of miceNotch pathway receptorsLgr5 stem cellsCellular differentiationNotch signalingNotch2 receptorMolecular approachesPathway receptorsNotch pathway inhibitionHuman organoidsEpithelial cell proliferationNotch inhibitionInhibition of Notch1Notch inhibitorsOrganoid growthCell proliferationNotch2
2012
Notch and disease: A growing field
Louvi A, Artavanis-Tsakonas S. Notch and disease: A growing field. Seminars In Cell And Developmental Biology 2012, 23: 473-480. PMID: 22373641, PMCID: PMC4369912, DOI: 10.1016/j.semcdb.2012.02.005.Peer-Reviewed Original ResearchConceptsCellular fate choicesAdult stem cellsInvolvement of NotchFate choiceNotch receptorsHuman diseasesNormal developmentPleiotropic fashionStem cellsRational therapeutic avenueBiologyTherapeutic avenuesBroad actionPathwayProfound involvementComplex controlExperimental systemNotchRelated pathologiesCellsReceptorsPathobiology
2011
Notch Lineages and Activity in Intestinal Stem Cells Determined by a New Set of Knock-In Mice
Fre S, Hannezo E, Sale S, Huyghe M, Lafkas D, Kissel H, Louvi A, Greve J, Louvard D, Artavanis-Tsakonas S. Notch Lineages and Activity in Intestinal Stem Cells Determined by a New Set of Knock-In Mice. PLOS ONE 2011, 6: e25785. PMID: 21991352, PMCID: PMC3185035, DOI: 10.1371/journal.pone.0025785.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBasic Helix-Loop-Helix Transcription FactorsCell DifferentiationCell LineageClone CellsEnterocytesGene Knock-In TechniquesGene TargetingHomeodomain ProteinsIntegrasesIntestinesKineticsMiceMice, Inbred C57BLMice, TransgenicMicrovilliMultipotent Stem CellsReceptors, NotchSequence Homology, Amino AcidSignal TransductionStem CellsTranscription Factor HES-1Transcription, Genetic