Samuel Steiner, PhD
Associate Research ScientistCards
About
Research
Publications
2022
Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System
Park D, Steiner S, Shao M, Roy CR, Liu J. Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System. Infection And Immunity 2022, 90: e00410-22. PMID: 36190257, PMCID: PMC9584302, DOI: 10.1128/iai.00410-22.Peer-Reviewed Original ResearchConceptsSmall cell variantLarge cell variantDot/Icm type IV secretion systemCell variantIntracellular replicationActive large cell variantHost cellsIntracellular bacterial pathogenType IV secretion systemDot/Icm T4SSObligate intracellular bacterial pathogenC. burnetiiCoxiella burnetiiBiphasic developmental cycleUnique biphasic developmental cycleInfectionNew host cellsSecretion systemBacterial pathogensInfectious formHost vacuoleBurnetiiMorphological changesCellsLater stages
2017
DNA Delivery and Genomic Integration into Mammalian Target Cells through Type IV A and B Secretion Systems of Human Pathogens
Guzmán-Herrador DL, Steiner S, Alperi A, González-Prieto C, Roy CR, Llosa M. DNA Delivery and Genomic Integration into Mammalian Target Cells through Type IV A and B Secretion Systems of Human Pathogens. Frontiers In Microbiology 2017, 8: 1503. PMID: 28878740, PMCID: PMC5572225, DOI: 10.3389/fmicb.2017.01503.Peer-Reviewed Original Research
2015
Cyclic di-GMP acts as a cell cycle oscillator to drive chromosome replication
Lori C, Ozaki S, Steiner S, Böhm R, Abel S, Dubey BN, Schirmer T, Hiller S, Jenal U. Cyclic di-GMP acts as a cell cycle oscillator to drive chromosome replication. Nature 2015, 523: 236-239. PMID: 25945741, DOI: 10.1038/nature14473.Peer-Reviewed Original Research
2012
Allosteric activation of exopolysaccharide synthesis through cyclic di‐GMP‐stimulated protein–protein interaction
Steiner S, Lori C, Boehm A, Jenal U. Allosteric activation of exopolysaccharide synthesis through cyclic di‐GMP‐stimulated protein–protein interaction. The EMBO Journal 2012, 32: 354-368. PMID: 23202856, PMCID: PMC3567491, DOI: 10.1038/emboj.2012.315.Peer-Reviewed Original ResearchConceptsProtein-protein interactionsSecond messenger cInner membrane componentsEscherichia coli biofilmsGMP receptorGMP signalingGMP bindsSynthesis machineryExtracellular matrix componentsMajor extracellular matrix componentsAllosteric activationExopolysaccharide synthesisExopolysaccharide matrixMembrane componentsColi biofilmsGlycosyltransferase activityBacterial pathogensMachineryN-acetylglucosaminePathogenic bacteriaMatrix componentsEnzyme activityGMP concentrationGMPBacteria
2009
Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress
Boehm A, Steiner S, Zaehringer F, Casanova A, Hamburger F, Ritz D, Keck W, Ackermann M, Schirmer T, Jenal U. Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress. Molecular Microbiology 2009, 72: 1500-1516. PMID: 19460094, DOI: 10.1111/j.1365-2958.2009.06739.x.Peer-Reviewed Original ResearchMeSH KeywordsAdhesins, BacterialAnti-Bacterial AgentsBeta-GlucansBiofilmsCyclic GMPEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialGuanosine TetraphosphatePhosphorus-Oxygen LyasesProtein BiosynthesisPyrophosphatasesRibosomesRNA Processing, Post-TranscriptionalSecond Messenger SystemsConceptsBiofilm inductionRibosome-targeting antibioticsNovel regulatory mechanismSecond messenger signalingSpecific derepressionBiosynthesis machineryRibosomal stressTranslational stressTranslation inhibitorsPpGpp levelsExposure of bacteriaMicrobial cellsRegulatory mechanismsAdhesin productionPolysaccharide adhesinBiofilm formationPpGppImportant synergistic roleMaximal inductionBiofilm controlMachineryBiofilm modelInductionGMPSubinhibitory concentrations