2004
Inhibition of alloresponse by a human trophoblast non-coding RNA suppressing class II transactivator promoter III and major histocompatibility class II expression in murine B-lymphocytes
Geirsson A, Bothwell AL, Hammond GL. Inhibition of alloresponse by a human trophoblast non-coding RNA suppressing class II transactivator promoter III and major histocompatibility class II expression in murine B-lymphocytes. The Journal Of Heart And Lung Transplantation 2004, 23: 1077-1081. PMID: 15454174, DOI: 10.1016/j.healun.2004.07.020.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, NorthernB-LymphocytesGenes, MHC Class IIHumansMiceNuclear ProteinsPromoter Regions, GeneticRNATrans-ActivatorsTransfectionTrophoblastsConceptsClass II expressionCIITA promoter IIIPromoter IIIMajor histocompatibilityClass II transactivator promoter IIIMajor histocompatibility class II expressionMHC class II expressionConstitutive class II expressionClass II transcriptsAllograft toleranceLymphocyte proliferationT lymphocytesAllogenic responseLymphocyte stimulationMurine B lymphocytesB cellsB lymphocytesClass IIFlow cytometryFunctional affectsConcurrent suppressionBp 152AlloresponseSignificant suppressionCIITA promoter
2003
Class II transactivator promoter activity is suppressed through regulation by a trophoblast noncoding RNA1
Geirsson A, Paliwal I, Lynch RJ, Bothwell AL, Hammond GL. Class II transactivator promoter activity is suppressed through regulation by a trophoblast noncoding RNA1. Transplantation 2003, 76: 387-394. PMID: 12883198, DOI: 10.1097/01.tp.0000073612.04525.46.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceDNA MethylationDNA, ComplementaryHeLa CellsHumansLuciferasesMajor Histocompatibility ComplexMolecular Sequence DataNuclear ProteinsNucleic Acid ConformationPromoter Regions, GeneticProtein Structure, TertiaryRNA, UntranslatedSuppression, GeneticTrans-ActivatorsTranscription, GeneticTransfectionTrophoblastsConceptsClass II expressionMHC class IICIITA promoter IVPromoter IVQuantitative polymerase chain reactionMajor histocompatibility complexAllograft tolerancePolymerase chain reactionFluorescence-activated cell sortingCIITA expressionHuman leukocyte antigen-DRClass IIMHC class II expressionClassic major histocompatibility complexChain reactionCell sortingPurposes of transplantationLack expressionHistocompatibility complexNoncoding RNAsII-deficient cellsSelective suppressionPromoter-reporter constructsCIITA promoterInhibitory domainHuman trophoblast noncoding RNA suppresses CIITA promoter III activity in murine B-lymphocytes
Geirsson A, Lynch RJ, Paliwal I, Bothwell AL, Hammond GL. Human trophoblast noncoding RNA suppresses CIITA promoter III activity in murine B-lymphocytes. Biochemical And Biophysical Research Communications 2003, 301: 718-724. PMID: 12565840, DOI: 10.1016/s0006-291x(03)00028-7.Peer-Reviewed Original ResearchConceptsClass II expressionCIITA expressionPromoter IIIClassical MHC antigensMHC class IICIITA promoter IIIAllograft toleranceMHC antigensMurine B lymphocytesFunctional assessmentB lymphocytesClass III-AkIII activityPromoter-reporter constructsTrophoblastCH27BlotTransient transfectionExpressionNorthern blotNegative trans-acting factorsNoncoding RNAsSuppressionTrans-acting factors