Longhui Zeng, PhD
Associate Research Scientist in Cell BiologyCards
About
Research
Publications
2025
CSF1R-CAR T cells induce CSF1R signaling and can promote target cell proliferation
Callahan A, Zhang X, Wang A, Mojumdar A, Zeng L, Su X, Salomon A. CSF1R-CAR T cells induce CSF1R signaling and can promote target cell proliferation. Science Signaling 2025, 18: eadv4112. PMID: 41218104, PMCID: PMC12684228, DOI: 10.1126/scisignal.adv4112.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CD19Cell Line, TumorCell ProliferationHumansImmunotherapy, AdoptiveLymphocyte Specific Protein Tyrosine Kinase p56(lck)Receptor, Macrophage Colony-Stimulating FactorReceptors, Chimeric AntigenReceptors, Granulocyte-Macrophage Colony-Stimulating FactorSignal TransductionT-LymphocytesTHP-1 CellsConceptsChimeric antigen receptorColony-stimulating factor-1Effector-to-target ratiosT cellsTHP-1 cellsTHP-1CSF1R signalingB-cell antigen CD19CD19 CAR-T cellsTarget cellsCell proliferationTHP-1 cell proliferationRefractory blood cancersCAR-T cellsColony-stimulating factor 1 receptorActivation of CSF1RT cell activationOptimal clinical efficacyTarget cell proliferationAntigen CD19Antigen receptorClinical efficacySmall molecule inhibitorsCell signaling responsesKinase activity783 Oncogenic PLCG1 drives aberrant intra- and intercellular T cell activation
Zeng L, Zhang X, Xiong Y, Sato K, Hajicek N, Kogure Y, Kataoka K, Ogawa S, Sondek J, Su X. 783 Oncogenic PLCG1 drives aberrant intra- and intercellular T cell activation. 2025, a891-a891. DOI: 10.1136/jitc-2025-sitc2025.0783.Peer-Reviewed Original ResearchIDR-induced CAR condensation improves the cytotoxicity of CAR-Ts against low-antigen cancers
Zhang X, Xiao Q, Zeng L, Hashmi F, Sato K, Hartwich T, Mansolf M, Yang-Hartwich Y, Su X. IDR-induced CAR condensation improves the cytotoxicity of CAR-Ts against low-antigen cancers. Nature Chemical Biology 2025, 1-13. PMID: 41023404, DOI: 10.1038/s41589-025-02031-x.Peer-Reviewed Original ResearchCAR-TChimeric antigen receptor (CAR)-T cell therapyCAR-T therapyCancer cells in vitroRelease of cytotoxic factorsCAR-T functionSolid tumor modelsAbsence of antigenLow antigen expressionCells in vitroMembrane-proximal signalingAntigen expressionAntigen sensitizationAntitumor effectCell therapyTumor modelIntractable cancerCytotoxic factorKilling activityBlood cancerCancerCancer targetLow antigenicityTherapyEfficacyHyperactive PLCG1 induces cell-autonomous and bystander T cell activation and drug resistance
Zeng L, Zhang X, Xiong Y, Sato K, Hajicek N, Kogure Y, Kataoka K, Ogawa S, Sondek J, Su X. Hyperactive PLCG1 induces cell-autonomous and bystander T cell activation and drug resistance. EMBO Reports 2025, 26: 4563-4586. PMID: 40796680, PMCID: PMC12457681, DOI: 10.1038/s44319-025-00546-x.Peer-Reviewed Original ResearchConceptsPhospholipase C-gamma 1T cell activationDrug resistanceNon-canonical signalingBystander T cell activationCutaneous T-cell lymphomaC gamma 1Adult T-cell leukemia/lymphomaResistance to vorinostatPro-survival phenotypeAlpha-smooth muscle actinT-cell lymphomaT-cell leukemia/lymphomaT cell physiologyT cell proliferationSmooth muscle actinCritical functionT cell survivalT cell signalingLAT condensatesCell adhesion moleculesCell-autonomousERK activationMutated genesERK signalingDesign of sensitive monospecific and bispecific synthetic chimeric T cell receptors for cancer therapy
Simon S, Bugos G, Prins R, Rajan A, Palani A, Heyer K, Stevens A, Zeng L, Thompson K, Atilla P, Price J, Kluesner M, Jaeger-Ruckstuhl C, Shabaneh T, Olson J, Su X, Riddell S. Design of sensitive monospecific and bispecific synthetic chimeric T cell receptors for cancer therapy. Nature Cancer 2025, 6: 647-665. PMID: 40097658, PMCID: PMC12037409, DOI: 10.1038/s43018-025-00927-0.Peer-Reviewed Original ResearchChimeric antigen receptorChimeric T cell receptorT cell receptorT cellsAdoptive transfer of T cells expressing chimeric antigen receptorsT cells expressing chimeric antigen receptorsChimeric antigen receptor T cellsBispecific CAR-T cellsPersistence of cancer cellsTCR-like signalsCAR-T cellsAntigen expression in vivoB-cell malignanciesMyeloma antigensExpression in vivoAdoptive transferEradicate tumorsAntigen sensitizationAntigen receptorFemale miceB cellsTumor heterogeneityPrevent relapseBispecific targetingImmune synapsesG3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration
Boraas L, Hu M, Martino P, Thornton L, Vejnar C, Zhen G, Zeng L, Parker D, Cox A, Giraldez A, Su X, Mayr C, Wang S, Nicoli S. G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration. Cell Reports 2025, 44: 115237. PMID: 39883578, PMCID: PMC11923778, DOI: 10.1016/j.celrep.2025.115237.Peer-Reviewed Original ResearchConceptsRNA-binding proteinsFocal adhesionsCell migrationStress granulesRNA-dependent mannerProtein mobilityFA proteinsRNA bindingDimerization domainSubcellular localizationRibonucleoprotein complexNon-stress conditionsFA sizeCell speedG3BP1RibonucleoproteinFA localizationBiological processesB-actinMRNAProteinCellsFA functionMigrationLocalization
2023
Biomolecular Condensation of SH2 Domain-Containing Proteins on Membranes
Zeng L, Su X. Biomolecular Condensation of SH2 Domain-Containing Proteins on Membranes. Methods In Molecular Biology 2023, 2705: 371-379. PMID: 37668985, DOI: 10.1007/978-1-0716-3393-9_20.Peer-Reviewed Original ResearchConceptsSH2 domainSH2 domain-containing proteinsDomain-containing proteinsT cell receptorReceptors/adaptorsReceptor pathwayContext of membraneEndomembrane systemMembrane receptor pathwaysSignal transductionBiomolecular condensationSpecific tyrosinePlasma membraneReconstitution systemGolgi apparatusEndoplasmic reticulumLiquid-liquid phase separationMultivalent interactionsCondensate formationProteinMembranePathwayPhosphotyrosineAssaysTransduction
2021
PLCγ1 promotes phase separation of T cell signaling components
Zeng L, Palaia I, Šarić A, Su X. PLCγ1 promotes phase separation of T cell signaling components. Journal Of Cell Biology 2021, 220: e202009154. PMID: 33929486, PMCID: PMC8094118, DOI: 10.1083/jcb.202009154.Peer-Reviewed Original ResearchConceptsTCR signal transductionT-cell receptor pathwayLiquid-like condensatesKey adaptor proteinLAT clusteringLAT complexSH2 domainAdaptor proteinPhosphatase CD45Signal transductionTCR pathwayPhospholipase Cγ1ERK activationProtein compositionBiophysical principlesPLCγ1Critical functionsReceptor pathwayPathwayLATMajor componentT cellsCellsActivationDephosphorylation
2019
B1 oligomerization regulates PML nuclear body biogenesis and leukemogenesis
Li Y, Ma X, Chen Z, Wu H, Wang P, Wu W, Cheng N, Zeng L, Zhang H, Cai X, Chen SJ, Chen Z, Meng G. B1 oligomerization regulates PML nuclear body biogenesis and leukemogenesis. Nature Communications 2019, 10: 3789. PMID: 31439836, PMCID: PMC6706441, DOI: 10.1038/s41467-019-11746-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarcinogenesisGene Knockout TechniquesHeLa CellsHumansLeukemia, Promyelocytic, AcuteMiceMice, TransgenicMutationOncogene Proteins, FusionPromyelocytic Leukemia ProteinProtein DomainsProtein MultimerizationRetinoic Acid Receptor alphaScattering, Small AngleSequence Analysis, RNASingle-Cell AnalysisSumoylationX-Ray DiffractionConceptsSingle-cell RNA sequencing analysisRNA sequencing analysisNuclear assemblyPML SUMOylationBiogenesisImportant regulatorOligomerization mechanismSequencing analysisUltracentrifugation analysisOligomerizationPML-RARαTransgenic miceLeukemogenesisGel filtrationSUMOylationSAXS characterizationTransactivationRegulatorProteinMechanismTetramerAssemblyF158Vivo studiesPML
2017
Structural basis of host recognition and biofilm formation by Salmonella Saf pili
Zeng L, Zhang L, Wang P, Meng G. Structural basis of host recognition and biofilm formation by Salmonella Saf pili. ELife 2017, 6: e28619. PMID: 29125121, PMCID: PMC5700814, DOI: 10.7554/elife.28619.Peer-Reviewed Original ResearchConceptsHost recognitionBiofilm formationNovel functionStructure-based mutantsSelf-association activityFunctional characterizationBiofilm formation assaysStructural basisBacterial aggregationFormation assaysCell aggregationPiliAdherence assaysColonizationSAXS characterizationAssaysMutantsSAFABacterial infectionsSAFDOligomerizationCrystal structureAggregationMechanismFormation