2023
Cell cycle controls long-range calcium signaling in the regenerating epidermis
Moore J, Bhaskar D, Gao F, Matte-Martone C, Du S, Lathrop E, Ganesan S, Shao L, Norris R, Sanz N, Annusver K, Kasper M, Cox A, Hendry C, Rieck B, Krishnaswamy S, Greco V. Cell cycle controls long-range calcium signaling in the regenerating epidermis. Journal Of Cell Biology 2023, 222: e202302095. PMID: 37102999, PMCID: PMC10140546, DOI: 10.1083/jcb.202302095.Peer-Reviewed Original Research
2022
Presynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9
Yang S, Park D, Manning L, Hill SE, Cao M, Xuan Z, Gonzalez I, Dong Y, Clark B, Shao L, Okeke I, Almoril-Porras A, Bai J, De Camilli P, Colón-Ramos DA. Presynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9. Neuron 2022, 110: 824-840.e10. PMID: 35065714, PMCID: PMC9017068, DOI: 10.1016/j.neuron.2021.12.031.Peer-Reviewed Original ResearchConceptsSynaptic vesicle cycleVesicle cyclePresynaptic autophagyAutophagosome biogenesisATG-9Only transmembrane proteinTrans-Golgi networkCellular degradation pathwayPresynaptic sitesActivity-dependent mannerTransmembrane proteinSynaptojanin 1Synaptic fociBiogenesisAutophagyNeuronal healthDegradation pathwayTraffickingPathwayParkinson's diseaseSynaptic activityNeuronal activityElegansSitesEndocytosis
2021
Differential adhesion regulates neurite placement via a retrograde zippering mechanism
Sengupta T, Koonce NL, Vázquez-Martínez N, Moyle MW, Duncan LH, Emerson SE, Han X, Shao L, Wu Y, Santella A, Fan L, Bao Z, Mohler W, Shroff H, Colón-Ramos DA. Differential adhesion regulates neurite placement via a retrograde zippering mechanism. ELife 2021, 10: e71171. PMID: 34783657, PMCID: PMC8843091, DOI: 10.7554/elife.71171.Peer-Reviewed Original ResearchConceptsDifferential adhesionDifferential adhesion mechanismsSYG-1SYG-2Developmental programEmbryonic developmentNeurite tipsZippering mechanismBiophysical principlesNeurite shaftSynaptic specificityBrain neuropilSingle neuriteLayers occursAlternate mechanismAdhesion mechanismExpressionNeuritesZipperingAdhesionMechanismOutgrowthStructural and developmental principles of neuropil assembly in C. elegans
Moyle MW, Barnes KM, Kuchroo M, Gonopolskiy A, Duncan LH, Sengupta T, Shao L, Guo M, Santella A, Christensen R, Kumar A, Wu Y, Moon KR, Wolf G, Krishnaswamy S, Bao Z, Shroff H, Mohler WA, Colón-Ramos DA. Structural and developmental principles of neuropil assembly in C. elegans. Nature 2021, 591: 99-104. PMID: 33627875, PMCID: PMC8385650, DOI: 10.1038/s41586-020-03169-5.Peer-Reviewed Original ResearchConceptsSpecific sensory organsNerve ringCaenorhabditis elegansC. elegansMuscle quadrantsNeuropil organizationDevelopmental principlesTissue organizationSensory organsBehavioral circuitsElegansPioneer neuronsCell positionDevelopmental sequenceStratified architectureTemporal progressionPrecise circuitsPacked neuronsUnique morphologyNeuronsSequenceOutgrowthAssemblyHierarchical developmentNeuropil
2020
Itaconate is an effector of a Rab GTPase cell-autonomous host defense pathway against Salmonella
Chen M, Sun H, Boot M, Shao L, Chang SJ, Wang W, Lam TT, Lara-Tejero M, Rego EH, Galán JE. Itaconate is an effector of a Rab GTPase cell-autonomous host defense pathway against Salmonella. Science 2020, 369: 450-455. PMID: 32703879, PMCID: PMC8020367, DOI: 10.1126/science.aaz1333.Peer-Reviewed Original Research
2019
Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution.
Duncan LH, Moyle MW, Shao L, Sengupta T, Ikegami R, Kumar A, Guo M, Christensen R, Santella A, Bao Z, Shroff H, Mohler W, Colón-Ramos DA. Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution. Journal Of Visualized Experiments 2019 PMID: 31233035, PMCID: PMC7255390, DOI: 10.3791/59533.Peer-Reviewed Original ResearchConceptsGene expressionC. elegans embryosCell lineage identityCell lineage analysisSingle-cell resolutionElegans embryosCaenorhabditis elegansC. elegansSelective plane illumination microscopyLight-sheet microscopyLineage analysisNematode embryosCell resolutionEntire nervous systemSubcellular levelOnly organismSubcellular resolutionElegansCellular originSingle cellsEmbryosIllumination microscopyEmbryogenesisMorphological dynamicsExpression
2011
Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution
Rego EH, Shao L, Macklin JJ, Winoto L, Johansson GA, Kamps-Hughes N, Davidson MW, Gustafsson MG. Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 109: e135-e143. PMID: 22160683, PMCID: PMC3271870, DOI: 10.1073/pnas.1107547108.Peer-Reviewed Original ResearchConceptsStructured-illumination microscopyUltralow light intensitiesSuperresolution imaging methodExcited statesFluorophore excited stateStructured illumination microscopyLight intensityRequired nonlinearityResolution extensionHigh light intensityActin cytoskeletonCellular structureReversible photoswitchingNuclear poresNonlinear responseIllumination intensitySuch nonlinear responsesPhotoswitchable proteinsSpatial resolutionFluorescent proteinBiological samplesSix-orderImaging methodMicroscopyPolystyrene beads