2023
Three-dimensional structured illumination microscopy with enhanced axial resolution
Li X, Wu Y, Su Y, Rey-Suarez I, Matthaeus C, Updegrove T, Wei Z, Zhang L, Sasaki H, Li Y, Guo M, Giannini J, Vishwasrao H, Chen J, Lee S, Shao L, Liu H, Ramamurthi K, Taraska J, Upadhyaya A, La Riviere P, Shroff H. Three-dimensional structured illumination microscopy with enhanced axial resolution. Nature Biotechnology 2023, 41: 1307-1319. PMID: 36702897, PMCID: PMC10497409, DOI: 10.1038/s41587-022-01651-1.Peer-Reviewed Original Research
2011
Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution
Rego EH, Shao L, Macklin JJ, Winoto L, Johansson GA, Kamps-Hughes N, Davidson MW, Gustafsson MG. Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 109: e135-e143. PMID: 22160683, PMCID: PMC3271870, DOI: 10.1073/pnas.1107547108.Peer-Reviewed Original ResearchConceptsStructured-illumination microscopyUltralow light intensitiesSuperresolution imaging methodExcited statesFluorophore excited stateStructured illumination microscopyLight intensityRequired nonlinearityResolution extensionHigh light intensityActin cytoskeletonCellular structureReversible photoswitchingNuclear poresNonlinear responseIllumination intensitySuch nonlinear responsesPhotoswitchable proteinsSpatial resolutionFluorescent proteinBiological samplesSix-orderImaging methodMicroscopyPolystyrene beads