2012
Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination
Fiolka R, Shao L, Rego EH, Davidson MW, Gustafsson MG. Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 5311-5315. PMID: 22431626, PMCID: PMC3325651, DOI: 10.1073/pnas.1119262109.Peer-Reviewed Original ResearchConceptsStructured-illumination microscopyOne-color excitationWide field natureDiffraction-limited resolutionLiquid crystal devicesSpecial fluorophoresCrystal devicesSIM setupExcitation wavelengthAcquisition speedHigh frame rateLight intensityFrame rateBiological structuresLive-cell imagingMultiple colorsMicroscopyHigh light intensityWavelengthLive cellsExcitationResolutionImaging
2011
Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution
Rego EH, Shao L, Macklin JJ, Winoto L, Johansson GA, Kamps-Hughes N, Davidson MW, Gustafsson MG. Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 109: e135-e143. PMID: 22160683, PMCID: PMC3271870, DOI: 10.1073/pnas.1107547108.Peer-Reviewed Original ResearchConceptsStructured-illumination microscopyUltralow light intensitiesSuperresolution imaging methodExcited statesFluorophore excited stateStructured illumination microscopyLight intensityRequired nonlinearityResolution extensionHigh light intensityActin cytoskeletonCellular structureReversible photoswitchingNuclear poresNonlinear responseIllumination intensitySuch nonlinear responsesPhotoswitchable proteinsSpatial resolutionFluorescent proteinBiological samplesSix-orderImaging methodMicroscopyPolystyrene beads