1999
Protein phosphatase 1 modulation of neostriatal AMPA channels: regulation by DARPP–32 and spinophilin
Yan Z, Hsieh–Wilson L, Feng J, Tomizawa K, Allen P, Fienberg A, Nairn A, Greengard P. Protein phosphatase 1 modulation of neostriatal AMPA channels: regulation by DARPP–32 and spinophilin. Nature Neuroscience 1999, 2: 13-17. PMID: 10195174, DOI: 10.1038/4516.Peer-Reviewed Original ResearchConceptsPP-1Protein phosphatase 1DARPP-32Distinct molecular mechanismsPhosphatase 1Molecular mechanismsAMPA receptor-mediated synaptic transmissionPostsynaptic densityAMPA channelsRegulationSynaptic plasticitySpinophilinNeostriatal neuronsPlasticityPhysiological evidenceGlutamate channelsSynaptic transmissionAMPA receptorsPhosphoproteinProteinMechanismBindingActivityModulationCatalytic activity
1998
The DARPP-32/protein phosphatase-1 cascade: a model for signal integration1Published on the World Wide Web on 22 January 1998.1
Greengard P, Nairn A, Girault J, Ouimet C, Snyder G, Fisone G, Allen P, Fienberg A, Nishi A. The DARPP-32/protein phosphatase-1 cascade: a model for signal integration1Published on the World Wide Web on 22 January 1998.1. Brain Research Reviews 1998, 26: 274-284. PMID: 9651542, DOI: 10.1016/s0165-0173(97)00057-x.Peer-Reviewed Original ResearchActions of Genistein on Cystic Fibrosis Transmembrane Conductance Regulator Channel Gating
Wang F, Zeltwanger S, Yang I, Nairn A, Hwang T. Actions of Genistein on Cystic Fibrosis Transmembrane Conductance Regulator Channel Gating. The Journal Of General Physiology 1998, 111: 477-490. PMID: 9482713, PMCID: PMC2217116, DOI: 10.1085/jgp.111.3.477.Peer-Reviewed Original ResearchConceptsCystic Fibrosis Transmembrane Conductance Regulator Channel GatingCFTR channelsSerine/threonine proteinTyrosine kinaseCystic fibrosis transmembrane conductance regulator (CFTR) channel activityDirect bindingHi-5 insect cellsCFTR channel currentsTyrosine phosphatase inhibitorMicroM genisteinProtein kinase AEffects of genisteinNonhydrolyzable ATP analogRecombinant CFTRProtein phosphatasePossible molecular mechanismsCFTR gatingInsect cellsPhosphatase inhibitorCalyculin ACFTR proteinAbsence of genisteinATP hydrolysisKinase ANIH3T3 cells
1992
Role of GTP-binding proteins in the regulation of mammalian cardiac chloride conductance.
Hwang T, Horie M, Nairn A, Gadsby D. Role of GTP-binding proteins in the regulation of mammalian cardiac chloride conductance. The Journal Of General Physiology 1992, 99: 465-489. PMID: 1375958, PMCID: PMC2219206, DOI: 10.1085/jgp.99.4.465.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAdenylate Cyclase ToxinAdrenergic beta-AgonistsAnimalsCarbacholCells, CulturedChloride ChannelsChloridesColforsinCyclic AMPGTP-Binding ProteinsGuanosine 5'-O-(3-Thiotriphosphate)Guinea PigsHeartHistamineIon ChannelsIsoproterenolMembrane ProteinsMyocardiumPertussis ToxinPhosphorylationPropranololReceptors, Adrenergic, betaReceptors, MuscarinicTime FactorsVirulence Factors, BordetellaConceptsProtein kinaseNonhydrolyzable GTP analogG proteinsCAMP-dependent protein kinaseG protein turnoverGTP-binding proteinsCl- conductanceAdenylyl cyclase activityCl- current activationGTP analogueMammalian cardiac myocytesGDP beta SSynthetic peptide inhibitorProtein turnoverStimulatory G proteinMammalian modelsPertussis toxinBeta SInhibitory G proteinBasal activationGTPPeptide inhibitorAdenylyl cyclaseCyclase activityProtein
1991
Enhancement of the Glutamate Response by cAMP-Dependent Protein Kinase in Hippocampal Neurons
Greengard P, Jen J, Nairn A, Stevens C. Enhancement of the Glutamate Response by cAMP-Dependent Protein Kinase in Hippocampal Neurons. Science 1991, 253: 1135-1138. PMID: 1716001, DOI: 10.1126/science.1716001.Peer-Reviewed Original ResearchConceptsProtein kinaseCAMP-dependent protein kinaseGlutamate receptor channelsMonophosphate-dependent protein kinaseReceptor channelsType glutamate receptor channelsAdenylate cyclase cascadeCultured hippocampal pyramidal neuronsSpontaneous excitatory postsynaptic currentsWhole-cell current responsesSingle-channel analysisNeuromodulatory regulationMammalian brainExcitatory postsynaptic currentsHippocampal pyramidal neuronsKinaseLong-term potentiationPyramidal neuronsPostsynaptic currentsGlutamate responseExcitatory neurotransmitterMean open timeHippocampal neuronsAdenylate cyclaseSynaptic events
1989
Chloride conductance regulated by cyclic AMP-dependent protein kinase in cardiac myocytes
Bahinski A, Nairn A, Greengard P, Gadsby D. Chloride conductance regulated by cyclic AMP-dependent protein kinase in cardiac myocytes. Nature 1989, 340: 718-721. PMID: 2475783, DOI: 10.1038/340718a0.Peer-Reviewed Original ResearchConceptsCyclic AMP-dependent protein kinaseAMP-dependent protein kinaseProtein kinaseChloride ion currentCatalytic subunitRegulatory proteinsKinase activationIon channelsKinaseChloride conductanceCalcium entrySingle-channel currentsCardiac myocytesCellsHeart cellsPhosphorylationAction potential repolarizationConductanceSubunitsProteinIntracellular dialysisMyocytesRegulationChannel currentsAdrenergic stimulationRegulation of Chloride Channels by Protein Kinase C in Normal and Cystic Fibrosis Airway Epithelia
Li M, McCann J, Anderson M, Clancy J, Liedtke C, Nairn A, Greengard P, Welsch M. Regulation of Chloride Channels by Protein Kinase C in Normal and Cystic Fibrosis Airway Epithelia. Science 1989, 244: 1353-1356. PMID: 2472006, DOI: 10.1126/science.2472006.Peer-Reviewed Original ResearchConceptsProtein kinase CChloride channelsKinase CApical membrane chloride channelMembrane chloride channelCystic fibrosis cellsMembrane proteinsCell-free membraneCystic fibrosis airway epitheliaChloride secretionIntact cellsPhorbol esterPhysiological statusDefective regulationAirway epithelial cellsEpithelial cellsCellsRegulationChannel inactivationCystic fibrosisActivationCalcium concentrationLow calcium concentrationsProteinAirway epithelium
1988
Cyclic AMP-dependent protein kinase opens chloride channels in normal but not cystic fibrosis airway epithelium
Li M, McCann J, Liedtket C, Nairn A, Greengard P, Welsh M. Cyclic AMP-dependent protein kinase opens chloride channels in normal but not cystic fibrosis airway epithelium. Nature 1988, 331: 358-360. PMID: 2448645, DOI: 10.1038/331358a0.Peer-Reviewed Original ResearchConceptsCAMP-dependent protein kinaseProtein kinaseRegulatory proteinsCl- channelsCyclic AMP-dependent protein kinaseAMP-dependent protein kinaseCommon lethal genetic diseaseNormal cellsLethal genetic diseaseApical Cl- channelsCatalytic subunitCell-free patchesCystic fibrosis airway epitheliaGenetic diseasesVariety of hormonesCF airway epitheliaCF cellsChloride channelsKinaseIntracellular levelsProteinAirway epitheliumCyclic AMPChloride secretionCells
1987
Cholecystokinin induces a decrease in Ca2+ current in snail neurons that appears to be mediated by protein kinase C
Hammond C, Paupardin-Tritsch D, Nairn A, Greengard P, Gerschenfeld H. Cholecystokinin induces a decrease in Ca2+ current in snail neurons that appears to be mediated by protein kinase C. Nature 1987, 325: 809-811. PMID: 2434859, DOI: 10.1038/325809a0.Peer-Reviewed Original ResearchConceptsProtein kinase CAction of noradrenalineDependent action potentialsKinase CIntracellular injectionSnail neuronsAction potentialsProtein kinaseCardiac muscleCholecystokininSnail Helix aspersaExcitable tissuesProtein kinase C.Low concentrationsCa2Cyclic GMP-dependent protein kinaseHelix aspersaInjectionGMP-dependent protein kinaseKinase C.KinaseNoradrenalineCCK8Neurons