Phosphorylation of connexin 32, a hepatocyte gap‐junction protein, by cAMP‐dependent protein kinase, protein kinase C and Ca2+/calmodulin‐dependent protein kinase II
SAEZ J, NAIRN A, CZERNIK A, SPRAY D, HERTZBERG E, GREENGARD P, BENNETT M. Phosphorylation of connexin 32, a hepatocyte gap‐junction protein, by cAMP‐dependent protein kinase, protein kinase C and Ca2+/calmodulin‐dependent protein kinase II. The FEBS Journal 1990, 192: 263-273. PMID: 2170122, DOI: 10.1111/j.1432-1033.1990.tb19223.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCalcium-Calmodulin-Dependent Protein KinasesConnexinsElectrophoresis, Gel, Two-DimensionalElectrophoresis, Polyacrylamide GelFemaleLiverMembrane ProteinsMolecular Sequence DataPeptide FragmentsPeptidesPhosphopeptidesPhosphorylationProtein Kinase CProtein KinasesRatsRats, Inbred StrainsConceptsProtein kinase CCAMP-dependent protein kinaseDependent protein kinase IIGap junction proteinPhosphopeptide mappingProtein kinaseSeryl residuesProtein kinase IICAMP-PKKinase IIKinase CCell typesConnexin 32PK IIPhosphoamino acid analysisDifferent gap junction proteinsSites of phosphorylationPhosphorylated synthetic peptideCAMP-PK activityGap junctionsAmino acid sequencingActivation of PKCDifferent cell typesPhysiological substratesSynthetic peptidesCalcium/calmodulin-dependent protein kinase II increases glutamate and noradrenaline release from synaptosomes
Nichols R, Sihra T, Czernik A, Nairn A, Greengard P. Calcium/calmodulin-dependent protein kinase II increases glutamate and noradrenaline release from synaptosomes. Nature 1990, 343: 647-651. PMID: 2154695, DOI: 10.1038/343647a0.Peer-Reviewed Original ResearchConceptsNeurotransmitter releaseRat brain synaptosomesSquid giant synapseRelease of neurotransmittersNoradrenaline releaseGlutamate releaseNerve terminalsBrain synaptosomesNervous systemUnidentified neurotransmitterGiant synapseDependent protein kinase IIVertebrate nervous systemRats calciumProtein kinase IINeurotransmittersInduced releasePK IISynaptosomesCalcium-dependent protein phosphorylationGlutamateKinase IIReleaseNoradrenalineInitial rate