2013
Characterization of CFTR High Expresser cells in the intestine
Jakab RL, Collaco AM, Ameen NA. Characterization of CFTR High Expresser cells in the intestine. AJP Gastrointestinal And Liver Physiology 2013, 305: g453-g465. PMID: 23868408, PMCID: PMC3761243, DOI: 10.1152/ajpgi.00094.2013.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAlkaline PhosphataseAnimalsCyclic AMPCystic Fibrosis Transmembrane Conductance RegulatorIntestinal MucosaIntestine, SmallMaleMicrovilliProtein TransportQa-SNARE ProteinsRatsRats, Sprague-DawleySodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSodium-Potassium-Exchanging ATPaseSolute Carrier Family 12, Member 2ConceptsCHE cellsNeighboring enterocytesVacuolar ATPase proton pumpCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelDouble-label immunofluorescence microscopyEightfold higher levelsBrush border membraneRegulatory factors NHERF1Crypt-villus axisCFTR abundanceSyntaxin 3Myosin-1aApical domain
2000
Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic
Ameen N, van Donselaar E, Posthuma G, de Jonge H, McLaughlin G, Geuze H, Marino C, Peters P. Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic. Histochemistry And Cell Biology 2000, 114: 219-228. PMID: 11083465, DOI: 10.1007/s004180000167.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorVesicle trafficSubcellular distributionVesicle insertionCAMP stimulationCAMP-activated chloride channelCryoimmunogold electron microscopyFibrosis transmembrane conductance regulatorApical plasma membraneTransmembrane conductance regulatorCultured intestinal cellsCFTR regulationCHE cellsPhysiologic roleVesicular compartmentsPlasma membraneApical redistributionConductance regulatorSubapical vesiclesCellular distributionChloride channelsSecretory cellsIntestinal cellsEpithelial cellsCells
1999
CFTR channel insertion to the apical surface in rat duodenal villus epithelial cells is upregulated by VIP in vivo
Ameen N, Martensson B, Bourguinon L, Marino C, Isenberg J, McLaughlin G. CFTR channel insertion to the apical surface in rat duodenal villus epithelial cells is upregulated by VIP in vivo. Journal Of Cell Science 1999, 112: 887-894. PMID: 10036238, DOI: 10.1242/jcs.112.6.887.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulator (CFTR) channelVillus epithelial cellsApical surfaceApical plasma membraneAnti-CFTR antibodiesQuantitative confocal microscopyNew protein synthesisApical membrane insertionIntestinal villus epithelial cellsEpithelial cellsMembrane CFTRMembrane insertionApical cytoskeletonNormal CFTRCHE cellsPlasma membraneAbsence of cycloheximideSubcellular redistributionCFTR channelsC-terminusCAMP stimulationIntracellular cAMP levelsProtein synthesisChannel insertionCFTR function