2017
High-speed super-resolution imaging of the proteins in fission yeast clathrin-mediated endocytic actin patches
Arasada R, Sayyad WA, Berro J, Pollard TD. High-speed super-resolution imaging of the proteins in fission yeast clathrin-mediated endocytic actin patches. Molecular Biology Of The Cell 2017, 29: mbc.e17-06-0415. PMID: 29212877, PMCID: PMC5996959, DOI: 10.1091/mbc.e17-06-0415.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActin-Related Protein 2-3 ComplexActinsAdaptor Proteins, Vesicular TransportCell MembraneClathrinClathrin-Coated VesiclesCytokinesisCytoplasmEndocytosisMicrofilament ProteinsMicroscopy, FluorescenceMyosin Type IProtein BindingSchizosaccharomycesSchizosaccharomyces pombe ProteinsConceptsActin filamentsLive fission yeast cellsEndocytic actin patchesSites of endocytosisFission yeast cellsNucleation-promoting factorsActin binding proteinsActin filament polymerizationCell surface receptorsActin patchesVesicle scissionFission yeastYeast clathrinArp2/3 complexSuperresolution localization microscopyTubule elongationFilament polymerizationRegulated mannerRegulatory proteinsMolecular mechanismsYeast cellsBinding proteinClathrinIndependent pathwaysCell surfaceSingle-molecule imaging of the BAR-domain protein Pil1p reveals filament-end dynamics
Lacy MM, Baddeley D, Berro J. Single-molecule imaging of the BAR-domain protein Pil1p reveals filament-end dynamics. Molecular Biology Of The Cell 2017, 28: 2251-2259. PMID: 28659415, PMCID: PMC5555653, DOI: 10.1091/mbc.e17-04-0238.Peer-Reviewed Original ResearchConceptsBAR domain proteinsSingle-molecule strategySingle-molecule imagingOligomeric filamentsFission yeastConventional fluorescence microscopyDynamic filamentsCytoplasmic faceMolecular assembliesLocalized recruitmentPlasma membraneEisosomesMacromolecular assembliesProtein dynamicsStable assemblyFluorescence microscopySuch assembliesPhysiological conditionsNanometer-scale featuresFirst direct observationProteinAssemblyHeterogeneous dynamicsPhotobleachingFilaments
2010
Quantitative Analysis of the Mechanism of Endocytic Actin Patch Assembly and Disassembly in Fission Yeast
Sirotkin V, Berro J, Macmillan K, Zhao L, Pollard TD. Quantitative Analysis of the Mechanism of Endocytic Actin Patch Assembly and Disassembly in Fission Yeast. Molecular Biology Of The Cell 2010, 21: 2894-2904. PMID: 20587778, PMCID: PMC2921122, DOI: 10.1091/mbc.e10-02-0157.Peer-Reviewed Original ResearchConceptsFission yeastActin assemblyActin patch assemblyEndocytic actin patchesEndocytic adaptor proteinActivators of Arp2/3Plasma membrane invaginationsRecruitment of dynaminQuantitative confocal microscopyActin filament fragmentationActin patchesArp2/3 complexPatch assemblyAdaptor proteinMembrane invaginationsCross-linked filamentsFluorescent proteinClathrin moleculesFilament fragmentationProteinConfocal microscopyYeastBranched networkIndividual patchesAssembly