Cross-regulations of two connected domains form a mechanical circuit for steady force transmission during clathrin-mediated endocytosis
Ren Y, Yang J, Fujita B, Zhang Y, Berro J. Cross-regulations of two connected domains form a mechanical circuit for steady force transmission during clathrin-mediated endocytosis. Cell Reports 2024, 43: 114725. PMID: 39276354, PMCID: PMC11476202, DOI: 10.1016/j.celrep.2024.114725.Peer-Reviewed Original ResearchClathrin-mediated endocytosisF-actinActin cytoskeletonFission yeast Schizosaccharomyces pombeYeast Schizosaccharomyces pombeCell adhesion complexAdhesion complexesMembrane localizationPN forcesStable bindingEnd4pCross-regulationCytoskeletonActinEndocytosisMembraneBindingMechanical forcesTalinTransmission of forcesThatchForce transmissionDomainCellsFissionCRISPR-Cas9 editing efficiency in fission yeast is not limited by homology search and is improved by combining gap-repair with fluoride selection
Fernandez R, Berro J. CRISPR-Cas9 editing efficiency in fission yeast is not limited by homology search and is improved by combining gap-repair with fluoride selection. MicroPublication Biology 2024, 2024: 10.17912/micropub.biology.001191. PMID: 38778900, PMCID: PMC11109758, DOI: 10.17912/micropub.biology.001191.Peer-Reviewed Original ResearchFission yeastEditing efficiencyHomology searchHomologous recombinational repair machineryDonor DNACRISPR-Cas9 protocolImprove editing efficiencyCRISPR-Cas9 editingModel organismsSelectable markerCut siteYeastGenome editingRepair machineryGap repairGenomeHomologyDNAEditing sequenceFissionLociHaloTagProteinSequenceMachinery