2016
Avoiding artefacts when counting polymerized actin in live cells with LifeAct fused to fluorescent proteins
Courtemanche N, Pollard TD, Chen Q. Avoiding artefacts when counting polymerized actin in live cells with LifeAct fused to fluorescent proteins. Nature Cell Biology 2016, 18: 676-683. PMID: 27159499, PMCID: PMC5509211, DOI: 10.1038/ncb3351.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActin Depolymerizing FactorsActinsAnimalsCell Cycle ProteinsCytokinesisCytoskeletal ProteinsEndocytosisProfilinsSchizosaccharomycesSchizosaccharomyces pombe Proteins
2013
Actin Filament Severing by Cofilin Dismantles Actin Patches and Produces Mother Filaments for New Patches
Chen Q, Pollard TD. Actin Filament Severing by Cofilin Dismantles Actin Patches and Produces Mother Filaments for New Patches. Current Biology 2013, 23: 1154-1162. PMID: 23727096, PMCID: PMC4131202, DOI: 10.1016/j.cub.2013.05.005.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActin Depolymerizing FactorsActin-Related Protein 2-3 ComplexEndocytosisMicrofilament ProteinsSchizosaccharomycesSchizosaccharomyces pombe ProteinsConceptsSites of endocytosisActin patchesWild-type cellsArp2/3 complexMother filamentActin filamentsAdaptor proteinActin polymerizationYeast cellsBranched actin filament networksEndocytic adaptor proteinActivators of Arp2/3Fission yeast cellsActin filament severingActin filament networkNew patchesEndocytic sitesQuantitative fluorescence microscopyMutant cofilinsMutant cellsNascent sitesFilament severingCofilinFilament networkEndocytosis