A combinatorial approach for robust transgene delivery and targeted expression in mammary gland for generating biotherapeutics in milk, bypassing germline gene integration
Ganguli N, Ganguli N, Chandra S, Choubey M, Sarkar D, Majumdar S. A combinatorial approach for robust transgene delivery and targeted expression in mammary gland for generating biotherapeutics in milk, bypassing germline gene integration. Applied Microbiology And Biotechnology 2018, 102: 6221-6234. PMID: 29855689, DOI: 10.1007/s00253-018-9094-2.Peer-Reviewed Original ResearchConceptsΒ-casein promoterGene integrationHemagglutinin-neuraminidaseEpithelial cell-specific expressionSpecific gene expressionCell-specific expressionMammary epithelial cellsEpithelial cellsNative genomeAnimal bioreactorsMammary glandMembrane fusionGene expressionSpecific expressionTransgene constructLuminal epithelial cellsMilk glandTargeted expressionViral membraneTransgeneFarmed animalsProtein expressionTherapeutic proteinsPromoterFusion factor