2019
Site‐specific phosphorylation of villin remodels the actin cytoskeleton to regulate Sendai viral glycoprotein‐mediated membrane fusion
Chandra S, Kumar M, Sharma N, Sarkar D. Site‐specific phosphorylation of villin remodels the actin cytoskeleton to regulate Sendai viral glycoprotein‐mediated membrane fusion. FEBS Letters 2019, 593: 1927-1943. PMID: 31183850, DOI: 10.1002/1873-3468.13477.Peer-Reviewed Original ResearchConceptsMembrane fusionVirus-host cell membrane fusionKey phosphorylation sitesQuantitative mass spectrometrySite-specific phosphorylationCell membrane fusionChinese hamster ovary cellsActin cytoskeletonPhosphorylation sitesHamster ovary cellsC-SrcTyrosine phosphorylationDependent phosphorylationCellular factorsCell fusionPhosphorylationOvary cellsVillinCritical roleVillin expressionSendai virosomesMass spectrometryFusionImportant roleCytoskeleton
2018
A combinatorial approach for robust transgene delivery and targeted expression in mammary gland for generating biotherapeutics in milk, bypassing germline gene integration
Ganguli N, Ganguli N, Chandra S, Choubey M, Sarkar D, Majumdar S. A combinatorial approach for robust transgene delivery and targeted expression in mammary gland for generating biotherapeutics in milk, bypassing germline gene integration. Applied Microbiology And Biotechnology 2018, 102: 6221-6234. PMID: 29855689, DOI: 10.1007/s00253-018-9094-2.Peer-Reviewed Original ResearchConceptsΒ-casein promoterGene integrationHemagglutinin-neuraminidaseEpithelial cell-specific expressionSpecific gene expressionCell-specific expressionMammary epithelial cellsEpithelial cellsNative genomeAnimal bioreactorsMammary glandMembrane fusionGene expressionSpecific expressionTransgene constructLuminal epithelial cellsMilk glandTargeted expressionViral membraneTransgeneFarmed animalsProtein expressionTherapeutic proteinsPromoterFusion factor
2017
Sendai virus recruits cellular villin to remodel actin cytoskeleton during fusion with hepatocytes
Chandra S, Kalaivani R, Kumar M, Srinivasan N, Sarkar D. Sendai virus recruits cellular villin to remodel actin cytoskeleton during fusion with hepatocytes. Molecular Biology Of The Cell 2017, 28: 3801-3814. PMID: 29074568, PMCID: PMC5739296, DOI: 10.1091/mbc.e17-06-0400.Peer-Reviewed Original ResearchConceptsMembrane fusionQuantitative mass spectrometryTwo-dimensional differentialViral envelope glycoproteinsSendai viral envelopesEarly molecular eventsHost cell proteinsActin cytoskeletonThreonine 206Actin dynamicsSendai virusAnimal cellsMolecular eventsCellular membranesHost cellsCell proteinsFusion-mediated deliveryNovel mechanismBiochemical analysisVillinEnvelope glycoproteinGel electrophoresisViral envelopeProteinViral entry