2022
Multimodal imaging of synaptic vesicles with a single probe
An SJ, Stagi M, Gould TJ, Wu Y, Mlodzianoski M, Rivera-Molina F, Toomre D, Strittmatter SM, De Camilli P, Bewersdorf J, Zenisek D. Multimodal imaging of synaptic vesicles with a single probe. Cell Reports Methods 2022, 2: 100199. PMID: 35497490, PMCID: PMC9046237, DOI: 10.1016/j.crmeth.2022.100199.Peer-Reviewed Original ResearchConceptsC2 domainSynaptic vesiclesSynaptic vesicle recyclingMembrane-binding C2 domainMultiple microscopy methodsEndocytic markersMembrane recyclingVesicle functionVesicle populationsCytosolic phospholipase ACell typesPhospholipase ADetectable tagMicroscopy modalitiesModular probesMultiple microscopy techniquesVesiclesComplete understandingDomainMicroscopy methodsMultiple levelsProbeAvailable probesMicroscopy techniquesPathway
2016
Extended‐Synaptotagmins as Lipid Transporters at ER‐PM Contact Sites
Reinisch K, Schauder C, Wu X, Saheki Y, Narayanaswamy P, Torta F, Wenk M, De Camilli P. Extended‐Synaptotagmins as Lipid Transporters at ER‐PM Contact Sites. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.115.2.Peer-Reviewed Original ResearchMembrane contact sitesSMP domainContact sitesExtended synaptotagminsEndoplasmic reticulumPlasma membraneER-PM contact sitesLipid transportLipid transferLipid-binding modulesER-PM tethersΒ-barrel structureExchange of lipidsERMES complexE-SytsProtein modulesProtein domainsVesicular transportC2 domainÅ resolutionLipid transportersHydrophobic residuesUehara Memorial FoundationSynaptotagmin 2Direct role