2001
Distinct functions and cooperative interaction of the subunits of the transporter associated with antigen processing (TAP)
Karttunen J, Lehner P, Gupta S, Hewitt E, Cresswell P. Distinct functions and cooperative interaction of the subunits of the transporter associated with antigen processing (TAP). Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 7431-7436. PMID: 11381133, PMCID: PMC34686, DOI: 10.1073/pnas.121180198.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmino Acid SubstitutionAnimalsATP Binding Cassette Transporter, Subfamily B, Member 2ATP Binding Cassette Transporter, Subfamily B, Member 3ATP-Binding Cassette TransportersAzidesBinding SitesCell LineHeLa CellsHumansLysineMajor Histocompatibility ComplexMutagenesis, Site-DirectedPeptide FragmentsPhotoaffinity LabelsProtein SubunitsRecombinant ProteinsTransfection
1998
Assembly of MHC class I molecules with biosynthesized endoplasmic reticulum-targeted peptides is inefficient in insect cells and can be enhanced by protease inhibitors.
Deng Y, Gibbs J, Bačík I, Porgador A, Copeman J, Lehner P, Ortmann B, Cresswell P, Bennink J, Yewdell J. Assembly of MHC class I molecules with biosynthesized endoplasmic reticulum-targeted peptides is inefficient in insect cells and can be enhanced by protease inhibitors. The Journal Of Immunology 1998, 161: 1677-85. PMID: 9712031, DOI: 10.4049/jimmunol.161.4.1677.Peer-Reviewed Original ResearchMeSH KeywordsAedesAnimalsAntibodies, MonoclonalAntiportersCell LineEndoplasmic ReticulumH-2 AntigensHeLa CellsHumansImmunoglobulinsLymphocyte ActivationMacromolecular SubstancesMembrane Transport ProteinsMiceOligopeptidesOvalbuminPeptide FragmentsProtease InhibitorsRecombinant ProteinsT-LymphocytesVaccinia virusConceptsInsect cellsEndoplasmic reticulumVertebrate cellsHuman cellsHuman tapasinVaccinia virus-mediated expressionCell surface expressionProtease inhibitorsInefficient assemblyKbMHC class IMouse betaInsectsEfficient assemblyImmediate precursorSurface expressionAntigenic peptidesHeavy chainClass IRecombinant vaccinia virusVirus-mediated expressionAssemblyExpressionCellsVaccinia virusInduction of transporter associated with antigen processing by interferon γ confers endothelial cell cytoprotection against natural killer-mediated lysis
Ayalon O, Hughes E, Cresswell P, Lee J, O’Donnell L, Pardi R, Bender J. Induction of transporter associated with antigen processing by interferon γ confers endothelial cell cytoprotection against natural killer-mediated lysis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 2435-2440. PMID: 9482903, PMCID: PMC19366, DOI: 10.1073/pnas.95.5.2435.Peer-Reviewed Original ResearchConceptsMHC IInduction of transportersEndothelial cellsNK-resistant cell linesNatural killer-mediated lysisMajor histocompatibility complex INK inhibitory receptorsNK effector cellsPretreatment of ECB-lymphoblastoid cell linesEC sensitivityDermal microvascular endothelial cellsMicrovascular endothelial cellsCell linesHuman umbilical vein endothelial cellsExpression/functionUmbilical vein endothelial cellsAdenoviral-mediated transductionVascular vulnerabilityVein endothelial cellsNK assaysEffector cellsEndothelial activationInhibitory receptorsDeficient targets
1990
The transport of class I major histocompatibility complex antigens is determined by sequences in the α1 and α2 protein domains
Alexander J, Payne J, Shigekawa B, Frelinger J, Cresswell P. The transport of class I major histocompatibility complex antigens is determined by sequences in the α1 and α2 protein domains. Immunogenetics 1990, 31: 169-178. PMID: 2318516, DOI: 10.1007/bf00211552.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsAntigens, SurfaceBiological TransportCell LineElectricityElectrophoresis, Gel, Two-DimensionalExonsFlow CytometryGlycosylationHistocompatibility Antigens Class IHLA-B7 AntigenHumansMiceMolecular Sequence DataProtein ConformationProtein Processing, Post-TranslationalRecombinant ProteinsT-LymphocytesTransfection