2012
Binding of the Heterogeneous Ribonucleoprotein K (hnRNP K) to the Epstein-Barr Virus Nuclear Antigen 2 (EBNA2) Enhances Viral LMP2A Expression
Gross H, Hennard C, Masouris I, Cassel C, Barth S, Stober-Grässer U, Mamiani A, Moritz B, Ostareck D, Ostareck-Lederer A, Neuenkirchen N, Fischer U, Deng W, Leonhardt H, Noessner E, Kremmer E, Grässer FA. Binding of the Heterogeneous Ribonucleoprotein K (hnRNP K) to the Epstein-Barr Virus Nuclear Antigen 2 (EBNA2) Enhances Viral LMP2A Expression. PLOS ONE 2012, 7: e42106. PMID: 22879910, PMCID: PMC3411732, DOI: 10.1371/journal.pone.0042106.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntibody SpecificityArginineCell LineDNAEpstein-Barr Virus InfectionsEpstein-Barr Virus Nuclear AntigensGene Expression Regulation, ViralHerpesvirus 4, HumanHeterogeneous-Nuclear Ribonucleoprotein KHumansImmunoprecipitationMethylationMolecular Sequence DataMultiprotein ComplexesMutant ProteinsPromoter Regions, GeneticProtein BindingProtein TransportRepetitive Sequences, Amino AcidSnRNP Core ProteinsTransfectionViral Matrix ProteinsViral ProteinsConceptsEpstein-Barr virus nuclear antigen 2Survival motor neuron (SMN) proteinHnRNP KHeterogeneous ribonucleoprotein KRG repeatsCellular proteinsSDMA-specific antibodyGel shift experimentsMotor neuron proteinHnRNP K.Cellular homologueCellular processesSequence motifsNp9 proteinsHuman endogenous retrovirus KArginine residuesNeuron proteinEBNA2 proteinShift experimentsLMP2A expressionNuclear antigen 2ProteinUnknown mechanismE. coliArginine-Glycine
2010
Arginine methylation in subunits of mammalian pre-mRNA cleavage factor I
Martin G, Ostareck-Lederer A, Chari A, Neuenkirchen N, Dettwiler S, Blank D, Rüegsegger U, Fischer U, Keller W. Arginine methylation in subunits of mammalian pre-mRNA cleavage factor I. RNA 2010, 16: 1646-1659. PMID: 20562214, PMCID: PMC2905762, DOI: 10.1261/rna.2164210.Peer-Reviewed Original ResearchConceptsProtein arginine methyltransferasesCleavage factor IMammalian cleavage factor IProcessing complexMessenger RNA precursorsPost-translational modificationsHeLa cell extractsRecombinant protein substratesSite of methylationS-adenosyl methionineBinding protein 1GAR motifArginine methylationArginine methyltransferasesRNA exportSecond methyltransferaseAsymmetric dimethylationRNA precursorsThird polypeptideC-terminusSuch enzymesArginine residuesCell extractsPolypeptide chainMethylation