1999
Cloning and characterization of mouse brush border myosin‐I in adult and embryonic intestine
Skowron J, Mooseker M. Cloning and characterization of mouse brush border myosin‐I in adult and embryonic intestine. Journal Of Experimental Zoology 1999, 283: 242-257. PMID: 9933937, DOI: 10.1002/(sici)1097-010x(19990215)283:3<242::aid-jez3>3.0.co;2-f.Peer-Reviewed Original ResearchConceptsBrush border myosinMicrovillar actin bundlesEmbryonic intestineSubcellular localization profileBasolateral membrane domainsGenomic clonesCalmodulin light chainsVertebrate speciesMembrane domainsActin corePlasma membraneKnockout strategiesActin bundlesImmunolocalization studiesIntestinal epithelial cellsGolgi apparatusNorthern analysisPrimary structureNorthern blotSitu localizationLocalization profilesRNA expressionEmbryosDirect targetingEmbryogenesis
1996
Human myosin-IXb, an unconventional myosin with a chimerin-like rho/rac GTPase-activating protein domain in its tail
Wirth J, Jensen K, Post P, Bement W, Mooseker M. Human myosin-IXb, an unconventional myosin with a chimerin-like rho/rac GTPase-activating protein domain in its tail. Journal Of Cell Science 1996, 109: 653-661. PMID: 8907710, DOI: 10.1242/jcs.109.3.653.Peer-Reviewed Original ResearchConceptsProtein domainsHL-60 cellsRho/Rac familyNovel N-terminal domainGTPase-activating protein (GAP) domainUnconventional myosin heavy chainRas-like G proteinsPrimary structurePutative actin-binding siteAmino acidsFull-length primary structureSkeletal muscle myosin IIN-terminal domainAmino acid extensionActin-binding siteProtein kinase CNorthern blot analysisMuscle myosin IIRac familyDifferent human tissuesUndifferentiated HL-60 cellsRegulatory domainPutative zincRac GTPaseCell periphery
1993
Cloning of a Secretory Gelsolin from Drosophila melanogaster
Heintzelman M, Frankel S, Artavanis-Tsakonas S, Mooseker M. Cloning of a Secretory Gelsolin from Drosophila melanogaster. Journal Of Molecular Biology 1993, 230: 709-716. PMID: 8386771, DOI: 10.1006/jmbi.1993.1191.Peer-Reviewed Original ResearchConceptsSecretory gelsolinAcid sequenceThird instar Drosophila larvaeDeduced amino acid sequenceFull-length complementary DNAInstar Drosophila larvaeClass of proteinsActin-binding proteinsAmino acid sequenceAmino acid residuesGelsolin sequenceThird chromosomeDrosophila melanogasterGelsolin familyCytoplasmic proteinsSignal peptideSingle geneDrosophila larvaeRepeat structureActin filamentsMonomeric actinAcid residuesBarbed endsPrimary structureNorthern blot
1991
Chapter 3 Structural and Functional Dissection of a Membrane-Bound Mechanoenzyme: Brush Border Myosin I
Mooseker M, Wolenski J, Coleman T, Hayden S, Cheney R, Espreafico E, Heintzelman M, Peterson M. Chapter 3 Structural and Functional Dissection of a Membrane-Bound Mechanoenzyme: Brush Border Myosin I. Current Topics In Membranes 1991, 38: 31-55. DOI: 10.1016/s0070-2161(08)60780-7.Peer-Reviewed Original ResearchBrush border myosin IBB myosin IMyosin ICalmodulin light chainsMicrovillar membraneProtein domain structureHeavy chainFunctional dissectionLight chainLinker proteinActin bindingMyosin I.Intestinal epithelial cellsActin filamentsMV membranePrimary structureApical membraneAcidic phospholipidsSubunit compositionTerminal webGlycoprotein bindsEpithelial cells