2002
MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells
Tyska M, Mooseker M. MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells. Biophysical Journal 2002, 82: 1869-1883. PMID: 11916846, PMCID: PMC1301984, DOI: 10.1016/s0006-3495(02)75537-9.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBiophysical PhenomenaBiophysicsCloning, MolecularCytoskeletonElectrophoresis, Polyacrylamide GelGreen Fluorescent ProteinsHumansKidneyKineticsLLC-PK1 CellsLuminescent ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceMicrovilliMyosin Type IPrecipitin TestsProtein Structure, TertiaryRecombinant Fusion ProteinsSubcellular FractionsSwineTime FactorsTransfectionConceptsTail domainLLC-PK1Actin core bundleKidney epithelial cell lineApical targetingActin dynamicsBrush borderActin turnoverGFP-actinBB populationsEpithelial cell lineActin domainsFluorescence recoveryCl4 cellsRapid turnoverApical surfaceMotor domainCore bundleATP depletionCell linesActinTurnoverCellsDomain
2000
Role of Actin and Myo2p in Polarized Secretion and Growth ofSaccharomyces cerevisiae
Karpova T, Reck-Peterson S, Elkind N, Mooseker M, Novick P, Cooper J. Role of Actin and Myo2p in Polarized Secretion and Growth ofSaccharomyces cerevisiae. Molecular Biology Of The Cell 2000, 11: 1727-1737. PMID: 10793147, PMCID: PMC14879, DOI: 10.1091/mbc.11.5.1727.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActinsBridged Bicyclo Compounds, HeterocyclicCarrier ProteinsCell DivisionCell PolarityFungal ProteinsGreen Fluorescent ProteinsLuminescent ProteinsMicroscopy, VideoMutationMyosin Heavy ChainsMyosin Type IIMyosin Type VRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSchizosaccharomyces pombe ProteinsThiazolesThiazolidinesConceptsActin patch polarizationCell surface growthPolarized secretionFilamentous actinCortical actin patchesClass V myosin Myo2pRole of actinTime-lapse video microscopyIndividual living cellsActin patchesPolarized growthActin cytoskeletonActin cablesMyo2-66Tail domainMyo2pSevere defectsLiving cellsOfSaccharomyces cerevisiaeMotor domainLatrunculinActinOverall growthVideo microscopyQuantitative assay
1998
Vesicle-associated brain myosin-V can be activated to catalyze actin-based transport
Evans L, Lee A, Bridgman P, Mooseker M. Vesicle-associated brain myosin-V can be activated to catalyze actin-based transport. Journal Of Cell Science 1998, 111: 2055-2066. PMID: 9645952, DOI: 10.1242/jcs.111.14.2055.Peer-Reviewed Original ResearchConceptsMyosin VVesicle proteinsTotal vesicle proteinSynaptic vesicle proteinsInitial fractionation stepSynaptic vesicle marker proteinActin transportBrain myosin-VOrganelle transportActin filament motilityOrganelle motorFunctional analysisVesicle fractionFunction-blocking antibodiesLocalization studiesMarker proteinsImmunoelectron microscopyMotility assaysMotor domainProteinVesiclesFilament motilityVesicle integrityActinVesicle surface
1997
Effects of shaker‐1 mutations on myosin‐VIIa protein and mRNA expression
Hasson T, Walsh J, Cable J, Mooseker M, Brown S, Steel K. Effects of shaker‐1 mutations on myosin‐VIIa protein and mRNA expression. Cytoskeleton 1997, 37: 127-138. PMID: 9186010, DOI: 10.1002/(sici)1097-0169(1997)37:2<127::aid-cm5>3.0.co;2-5.Peer-Reviewed Original ResearchConceptsShaker-1 mutationsWild-type levelsNorthern blot analysisMammalian diseasesActin cytoskeletonMyosin VIIaShaker-1 miceGene expressionUnconventional myosinMRNA expressionProteinMyosin-VIIa mutationsImmunoblot analysisMotor domainRange of expressionMutationsBlot analysisAllelesProtein expressionTissue functionExpressionLife spanSH1Inner earLight microscopic level