1999
Cloning and characterization of mouse brush border myosin‐I in adult and embryonic intestine
Skowron J, Mooseker M. Cloning and characterization of mouse brush border myosin‐I in adult and embryonic intestine. Journal Of Experimental Zoology 1999, 283: 242-257. PMID: 9933937, DOI: 10.1002/(sici)1097-010x(19990215)283:3<242::aid-jez3>3.0.co;2-f.Peer-Reviewed Original ResearchConceptsBrush border myosinMicrovillar actin bundlesEmbryonic intestineSubcellular localization profileBasolateral membrane domainsGenomic clonesCalmodulin light chainsVertebrate speciesMembrane domainsActin corePlasma membraneKnockout strategiesActin bundlesImmunolocalization studiesIntestinal epithelial cellsGolgi apparatusNorthern analysisPrimary structureNorthern blotSitu localizationLocalization profilesRNA expressionEmbryosDirect targetingEmbryogenesis
1993
An in vitro model for the analysis of intestinal brush border assembly I. Ultrastructural analysis of cell contact-induced brush border assembly in Caco-2BBe cells
Peterson M, Mooseker M. An in vitro model for the analysis of intestinal brush border assembly I. Ultrastructural analysis of cell contact-induced brush border assembly in Caco-2BBe cells. Journal Of Cell Science 1993, 105: 445-460. PMID: 8408276, DOI: 10.1242/jcs.105.2.445.Peer-Reviewed Original ResearchConceptsBrush border assemblyCaco-2BBe cellsIntestinal epithelial cellsMicrovillar densityActin core bundleEpithelial cells
1992
Localization of capping protein in chicken epithelial cells by immunofluorescence and biochemical fractionation.
Schafer D, Mooseker M, Cooper J. Localization of capping protein in chicken epithelial cells by immunofluorescence and biochemical fractionation. Journal Of Cell Biology 1992, 118: 335-346. PMID: 1629237, PMCID: PMC2290044, DOI: 10.1083/jcb.118.2.335.Peer-Reviewed Original ResearchMeSH KeywordsActin Depolymerizing FactorsActinsAnimalsAntibodiesCells, CulturedChick EmbryoChickensCochleaDestrinElectrophoresis, Polyacrylamide GelEpithelial CellsEpitheliumFluorescent Antibody TechniqueIntestinesKidneyMacromolecular SubstancesMicrofilament ProteinsMicrovilliMusclesPigment Epithelium of EyeSubcellular FractionsConceptsIntestinal epithelial cellsNuclei of cellsBiochemical fractionationJunctional complexesActin filamentsCell-cell junctional complexesSingle intestinal epithelial cellsBarbed endsEpithelial cellsSensory epitheliumCell-cell contactIntact intestinal epithelial cellsAffinity-purified polyclonal antibodiesProtein bindsCapping proteinIntestinal epitheliumZonula adherensCell junctionsChick embryo kidney cellsPattern coincidentEmbryo kidney cellsProteinConfocal microscopyKidney cellsIsolated intestinal epithelial cells
1991
Chapter 3 Structural and Functional Dissection of a Membrane-Bound Mechanoenzyme: Brush Border Myosin I
Mooseker M, Wolenski J, Coleman T, Hayden S, Cheney R, Espreafico E, Heintzelman M, Peterson M. Chapter 3 Structural and Functional Dissection of a Membrane-Bound Mechanoenzyme: Brush Border Myosin I. Current Topics In Membranes 1991, 38: 31-55. DOI: 10.1016/s0070-2161(08)60780-7.Peer-Reviewed Original ResearchBrush border myosin IBB myosin IMyosin ICalmodulin light chainsMicrovillar membraneProtein domain structureHeavy chainFunctional dissectionLight chainLinker proteinActin bindingMyosin I.Intestinal epithelial cellsActin filamentsMV membranePrimary structureApical membraneAcidic phospholipidsSubunit compositionTerminal webGlycoprotein bindsEpithelial cells
1990
Binding of brush border myosin I to phospholipid vesicles.
Hayden S, Wolenski J, Mooseker M. Binding of brush border myosin I to phospholipid vesicles. Journal Of Cell Biology 1990, 111: 443-451. PMID: 2143194, PMCID: PMC2116197, DOI: 10.1083/jcb.111.2.443.Peer-Reviewed Original ResearchConceptsBB myosin IMyosin IBrush border myosin IMyosin I heavy chainMembrane-binding domainActin filament corePhospholipid vesiclesATP-sensitive mannerPlasma membraneIntestinal epithelial cellsF-actinMembrane interactionsPhosphatidylglycerol vesiclesFree proteinCOOH-terminalAnionic phospholipidsImmunoblot analysisVesiclesEpithelial cellsImmunoblot stainingM. SimilarHeavy chainActinStructural informationNeutral phospholipids
1983
Regulation of Cytoskeletal Structure and Contractility in the Brush Border
Mooseker M, Keller T, Hirokawa N. Regulation of Cytoskeletal Structure and Contractility in the Brush Border. Novartis Foundation Symposia 1983, 95: 195-215. PMID: 6552204, DOI: 10.1002/9780470720769.ch12.Peer-Reviewed Original ResearchConceptsIntestinal epithelial cellsBrush borderEpithelial cellsRat brush bordersIsometric contractionFree Ca2ContractilityBrush border proteinsRegulation of forceContractionChicken intestineAnalysis of Ca2Light chainCa2Microvillar rootletsActin-severing proteinBrush border myosinPresence of Ca2Circumferential bundlesCalmodulin functionTerminal web regionCalciumCellsPhosphorylationCytoskeletal structures
1982
Ca++-calmodulin-dependent phosphorylation of myosin, and its role in brush border contraction in vitro.
Keller T, Mooseker M. Ca++-calmodulin-dependent phosphorylation of myosin, and its role in brush border contraction in vitro. Journal Of Cell Biology 1982, 95: 943-959. PMID: 6897550, PMCID: PMC2112925, DOI: 10.1083/jcb.95.3.943.Peer-Reviewed Original ResearchConceptsBrush border contractionBrush borderIntestinal epithelial cellsEpithelial cellsCalmodulin activityBrush border proteinsMyosin light chain kinaseContractionDegrees CLight chain kinaseLight chainCalmodulin-dependent phosphorylationBrush border myosinPhosphorylationDalton light chainChain kinaseTerminal webChapter 8 The Brush Border of Intestinal Epithelium: A Model System for Analysis of Cell-Surface Architecture and Motility
Mooseker M, Howe C. Chapter 8 The Brush Border of Intestinal Epithelium: A Model System for Analysis of Cell-Surface Architecture and Motility. Methods In Cell Biology 1982, 25: 143-174. PMID: 7109959, DOI: 10.1016/s0091-679x(08)61424-7.Chapters
1980
Regulation of microvillus structure: calcium-dependent solation and cross-linking of actin filaments in the microvilli of intestinal epithelial cells.
Mooseker M, Graves T, Wharton K, Falco N, Howe C. Regulation of microvillus structure: calcium-dependent solation and cross-linking of actin filaments in the microvilli of intestinal epithelial cells. Journal Of Cell Biology 1980, 87: 809-822. PMID: 6893989, PMCID: PMC2110803, DOI: 10.1083/jcb.87.3.809.Peer-Reviewed Original ResearchConceptsIntestinal epithelial cellsEpithelial cellsCore proteinLong-term centrifugationReversible disruption