1995
Characterization of Myosin-IA and Myosin-IB, Two Unconventional Myosins Associated with theDrosophilaBrush Border Cytoskeleton
Morgan N, Heintzelman M, Mooseker M. Characterization of Myosin-IA and Myosin-IB, Two Unconventional Myosins Associated with theDrosophilaBrush Border Cytoskeleton. Developmental Biology 1995, 172: 51-71. PMID: 7589814, DOI: 10.1006/dbio.1995.0005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCloning, MolecularCytoskeletonDrosophila melanogasterElectrophoresis, Polyacrylamide GelEmbryo, NonmammalianFemaleGene Expression RegulationImmunoblottingImmunohistochemistryLarvaMicroscopy, ElectronMicroscopy, ImmunoelectronMicrovilliMyosinsOogenesisOvumPupaRecombinant ProteinsConceptsMyosin IBAdult gutATP-dependent extractionMyosin IASomatic follicle cellsExogenous F-actinBrush border cytoskeletonNovel unconventional myosinDrosophila melanogasterMicrovillus assemblyEgg chambersApical domainMicrovillar componentsMicrovillar domainApical microvillar domainBasolateral domainExpression patternsUnconventional myosinF-actinSubcellular distributionFollicle cellsFunctional myosinDifferentiated enterocytesBiochemical propertiesBrush border
1992
Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2
Peterson M, Mooseker M. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. Journal Of Cell Science 1992, 102: 581-600. PMID: 1506435, DOI: 10.1242/jcs.102.3.581.Peer-Reviewed Original ResearchConceptsMyosin IBB myosin IC2BBe cellsATP-dependent associationBrush border cytoskeletonApical brush borderHeavy chainDomain-specific monoclonal antibodiesBrush borderFilter-grown monolayersCaco-2Cell line Caco-2Human colonic epithelial cellsHuman intestinal cell lineMyosin I.Myosin IIIntestinal cell linePhysical associationLaser scanning confocal microscopyProtein villinCytoskeletonCell culture modelCell typesPattern of distributionVivo structureThe molecular architecture of an insect midgut brush border cytoskeleton.
Bonfanti P, Colombo A, Heintzelman M, Mooseker M, Camatini M. The molecular architecture of an insect midgut brush border cytoskeleton. European Journal Of Cell Biology 1992, 57: 298-307. PMID: 1511705.Peer-Reviewed Original ResearchConceptsTwo-dimensional gel analysisMolecular architectureMultiple isoelectric variantsBrush border cytoskeletonLepidopteran larvaeBB myosin IVertebrate intestineBrush borderMyosin IOrganelle exclusionHeavy meromyosin decorationCytoskeletal apparatusMyosin IINonmuscle cellsActin filamentsCytoskeletonIsoelectric variantsGel analysisInstar larvaeMajor isoformsStructural organizationRhodamine phalloidinCore proteinIntestinal brush borderMidgut