Longfei Liu
Associate Research Scientist in Cell BiologyAbout
Research
Publications
2023
Molecular Lithography on Silicon Wafers Guided by Porous, Extended Arrays of Small DNA Tiles
Mao D, Liu L, Zhang C, Liu H, Mao C. Molecular Lithography on Silicon Wafers Guided by Porous, Extended Arrays of Small DNA Tiles. Langmuir 2023, 39: 11782-11787. PMID: 37562139, DOI: 10.1021/acs.langmuir.3c01422.Peer-Reviewed Original ResearchConceptsE-beam lithographyDNA nanostructuresCost-effective fabrication methodSilicon wafersDNA origami structuresTile-based DNAMolecular lithographyCorresponding nanostructuresFabrication methodSmall DNA tilesNanostructuresFeature sizeLithographyOrigami structuresHF etchingDNA tilesWafersExtended arrayArrayLarge areasEtchingHereinSerial methodTemplateSizeSurface-assisted self-assembly of 2D, DNA binary crystals
Liu L, Mao D, Li Z, Zheng M, He K, Mao C. Surface-assisted self-assembly of 2D, DNA binary crystals. Nanoscale 2023, 15: 9941-9945. PMID: 37249191, DOI: 10.1039/d3nr01187a.Peer-Reviewed Original ResearchConceptsSolid surfaceTwo-dimensional nanoarraysDifferent adsorption strengthsComparable molecular weightAdsorption strengthDifferent tilesDifferential adsorptionComplicated nanostructuresMolar ratioMolecular weightBinary crystalsPowerful methodStructural complexityNanoarraysAdsorptionNanostructuresSurfaceEffective molar ratioHereinTile concentrations
2022
Functionalized DNA-Origami-Protein Nanopores Generate Large Transmembrane Channels with Programmable Size-Selectivity
Shen Q, Xiong Q, Zhou K, Feng Q, Liu L, Tian T, Wu C, Xiong Y, Melia T, Lusk C, Lin C. Functionalized DNA-Origami-Protein Nanopores Generate Large Transmembrane Channels with Programmable Size-Selectivity. Journal Of The American Chemical Society 2022, 145: 1292-1300. PMID: 36577119, PMCID: PMC9852090, DOI: 10.1021/jacs.2c11226.Peer-Reviewed Original ResearchConceptsExchange of macromoleculesCholesterol-rich membranesHybrid nanoporesSynthetic biologyBiophysical toolsSynthetic cellsTransmembrane channelsTransmembrane nanoporesDNA ringsProtein nanoporeCell membraneBacterial toxinsDNA origami techniqueLipid membranesAnalytical chemistryMacromolecule sizeDNA origamiMembraneProgrammable sizeNanoporesSized poresNucleoporinsAverage inner diameterCellsPneumolysinActuating tension-loaded DNA clamps drives membrane tubulation
Liu L, Xiong Q, Xie C, Pincet F, Lin C. Actuating tension-loaded DNA clamps drives membrane tubulation. Science Advances 2022, 8: eadd1830. PMID: 36223466, PMCID: PMC9555772, DOI: 10.1126/sciadv.add1830.Peer-Reviewed Original ResearchConceptsDNA clampMembrane tubulationMembrane dynamicsMembrane-remodeling eventsVesicle tubulationConformational changesSpatiotemporal controlDNA signalsCell membraneDNA nanostructuresTubulationMembrane deformationClosed stateOpen stateSelf-assembled DNA nanostructuresOrganismsProteinMembrane tubeArtificial systemsTube widthMembraneDynamics
2021
Kinetic DNA Self-Assembly: Simultaneously Co-folding Complementary DNA Strands into Identical Nanostructures
Zheng M, Li Z, Liu L, Li M, Paluzzi V, Choi J, Mao C. Kinetic DNA Self-Assembly: Simultaneously Co-folding Complementary DNA Strands into Identical Nanostructures. Journal Of The American Chemical Society 2021, 143: 20363-20367. PMID: 34808053, DOI: 10.1021/jacs.1c09925.Peer-Reviewed Original ResearchConceptsDNA nanostructuresAtomic force microscopy imagingLimited production yieldForce microscopy imagingLong DNA strandsComplementary DNA strandsDNA strandsDNA origamiEnzymatic reactionsIdentical nanostructuresNanostructuresLarge-scale productionDNA duplexConvenient methodInteraction kineticsMicroscopy imagingDuplex DNAComplementary strandSame solution5′-Phosphorylation Strengthens Sticky-End Cohesions
Li Z, Zheng M, Liu L, Seeman N, Mao C. 5′-Phosphorylation Strengthens Sticky-End Cohesions. Journal Of The American Chemical Society 2021, 143: 14987-14991. PMID: 34516099, DOI: 10.1021/jacs.1c07279.Peer-Reviewed Original ResearchSticky-ended cohesionDNA crystalsSelf-assembly kineticsFree energy calculationsNucleic acid nanotechnologyMolecular mechanicsChemical modificationNatural DNACrystal morphologyEnergy calculationsCrystal growthSynthetic DNACrystallization processCrystallization kineticsCrystalsKineticsPotential differenceNanotechnologyHereinMorphologyCalculationsSorting sub-150-nm liposomes of distinct sizes by DNA-brick-assisted centrifugation
Yang Y, Wu Z, Wang L, Zhou K, Xia K, Xiong Q, Liu L, Zhang Z, Chapman ER, Xiong Y, Melia TJ, Karatekin E, Gu H, Lin C. Sorting sub-150-nm liposomes of distinct sizes by DNA-brick-assisted centrifugation. Nature Chemistry 2021, 13: 335-342. PMID: 33785892, PMCID: PMC8049973, DOI: 10.1038/s41557-021-00667-5.Peer-Reviewed Original ResearchDNA origami
Dey S, Fan C, Gothelf K, Li J, Lin C, Liu L, Liu N, Nijenhuis M, Saccà B, Simmel F, Yan H, Zhan P. DNA origami. Nature Reviews Methods Primers 2021, 1: 13. DOI: 10.1038/s43586-020-00009-8.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsDNA origami technologyOrigami technologyDNA origami techniqueNear-atomic precisionDNA nanotechnologyOrigami techniqueDNA origamiDrug deliveryOrigami structuresMolecular machinesOrigami designBiological materialsLiving cellsSynthesisVivo synthesisNanophotonicsNanotechnologyNanofabricationNanostructuresNanoelectronicsBioimagingScale of productionFabricationTechnologyFunctionalization
2020
A poly(thymine)–melamine duplex for the assembly of DNA nanomaterials
Li Q, Zhao J, Liu L, Jonchhe S, Rizzuto F, Mandal S, He H, Wei S, Sleiman H, Mao H, Mao C. A poly(thymine)–melamine duplex for the assembly of DNA nanomaterials. Nature Materials 2020, 19: 1012-1018. PMID: 32661383, PMCID: PMC7732259, DOI: 10.1038/s41563-020-0728-2.Peer-Reviewed Original ResearchConceptsHydrogen-bonding motifsPresence of melamineHydrogen bonding faceX-ray crystallographyAdenine-thymine base pairsDNA base pairingDNA duplex structureDNA nanomaterialsHydrogen bondsDNA nanotechnologyRight-handed duplexSensitive detectionHelical columnsSmall moleculesMelamineStrand displacementBase pairingMechanical strengthDuplexDuplex structureThymine residuesPolymersNanomaterialsCrystallographyBondsAssembly of a DNA Origami Chinese Knot by Only 15% of the Staple Strands
He K, Li Z, Liu L, Zheng M, Mao C. Assembly of a DNA Origami Chinese Knot by Only 15% of the Staple Strands. ChemBioChem 2020, 21: 2132-2136. PMID: 32196869, DOI: 10.1002/cbic.202000106.Peer-Reviewed Original Research