2020
Contribution of increasing plasma membrane to the energetic cost of early zebrafish embryogenesis
Rodenfels J, Sartori P, Golfier S, Nagendra K, Neugebauer K, Howard J. Contribution of increasing plasma membrane to the energetic cost of early zebrafish embryogenesis. Molecular Biology Of The Cell 2020, 31: 520-526. PMID: 32049586, PMCID: PMC7202076, DOI: 10.1091/mbc.e19-09-0529.Peer-Reviewed Original ResearchConceptsPlasma membraneEarly zebrafish embryogenesisEnergetic costSpecific developmental eventsTotal plasma membraneCell proliferationTwo-cell stageZebra fish embryosZebrafish embryogenesisTotal cell surface areaDNA replicationCell divisionEarly embryosDevelopmental eventsCleavage divisionsCell surface areaTotal cell numberCleavage stagesEmbryo volumeEmbryosCell numberMembraneTotal energy budgetProliferationEmbryogenesis
2018
RNA tales – how embryos read and discard messages from mom
Despic V, Neugebauer KM. RNA tales – how embryos read and discard messages from mom. Journal Of Cell Science 2018, 131: jcs201996. PMID: 29467249, DOI: 10.1242/jcs.201996.Peer-Reviewed Original ResearchConceptsZygotic genomePost-transcriptional regulatory pathwaysMaternal mRNA clearanceMaternal mRNA degradationEarly developmentContribution of microRNAsZygotic transitionMRNA clearanceRNA regulationMRNA decayCellular contextHigh-throughput methodZebrafish embryosFemale gametesMaternal mRNAsMolecular playersMRNA degradationRNA modificationsMolecular principlesRegulatory pathwaysGenomeLater stepsMZTSilent genomeEmbryos
2017
Analysis of RNA-protein interactions in vertebrate embryos using UV crosslinking approaches
Despic V, Dejung M, Butter F, Neugebauer KM. Analysis of RNA-protein interactions in vertebrate embryos using UV crosslinking approaches. Methods 2017, 126: 44-53. PMID: 28734934, DOI: 10.1016/j.ymeth.2017.07.013.Peer-Reviewed Original ResearchConceptsNumber of RBPsRNA-protein interactionsUnique biological contextZebrafish Danio rerioRegulated gene expressionInteractome captureVertebrate embryosDanio rerioRNA-seqCellular RNAGene expressionBiological contextRBPsRNAProteinGenomeRerioCrosslinking approachOrganismsEmbryosMRNAAnnotationExpressionVast frontierVivoPurification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos
Heyn P, Neugebauer KM. Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos. Methods In Molecular Biology 2017, 1605: 121-131. PMID: 28456961, DOI: 10.1007/978-1-4939-6988-3_8.Peer-Reviewed Original Research
2010
Coilin-dependent snRNP assembly is essential for zebrafish embryogenesis
Strzelecka M, Trowitzsch S, Weber G, Lührmann R, Oates AC, Neugebauer KM. Coilin-dependent snRNP assembly is essential for zebrafish embryogenesis. Nature Structural & Molecular Biology 2010, 17: 403-409. PMID: 20357773, DOI: 10.1038/nsmb.1783.Peer-Reviewed Original ResearchDynamic control of Cajal body number during zebrafish embryogenesis
Strzelecka M, Oates AC, Neugebauer KM. Dynamic control of Cajal body number during zebrafish embryogenesis. Nucleus 2010, 1: 96-108. PMID: 21327108, PMCID: PMC3035118, DOI: 10.4161/nucl.1.1.10680.Peer-Reviewed Original ResearchConceptsCajal bodiesEnd processing factorsGene expressionZygotic genome activationZygotic gene expressionRNA processing machineryDistinct nuclear bodiesMRNA splicing machineryCajal body numberLive-cell imagingCB numberHours of developmentGenome activationZebrafish embryogenesisNuclear subcompartmentsSnRNP biogenesisSplicing machineryZebrafish embryosCell divisionNuclear bodiesCB assemblyEmbryonic cellsMaternal contributionProcessing machineryEmbryos