Combined Electron Transfer Dissociation–Collision-Induced Dissociation Fragmentation in the Mass Spectrometric Distinction of Leucine, Isoleucine, and Hydroxyproline Residues in Peptide Natural Products
Gupta K, Kumar M, Chandrashekara K, Krishnan KS, Balaram P. Combined Electron Transfer Dissociation–Collision-Induced Dissociation Fragmentation in the Mass Spectrometric Distinction of Leucine, Isoleucine, and Hydroxyproline Residues in Peptide Natural Products. Journal Of Proteome Research 2011, 11: 515-522. PMID: 22111579, DOI: 10.1021/pr200091v.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBiological ProductsHydroxyprolineIsoleucineLeucineMass SpectrometryMelittenMolecular Sequence DataPeptaibolsPeptidesSequence Analysis, ProteinWasp VenomsConceptsIsobaric residuesMass spectrometric peptide sequencingNatural peptide librariesPeptide natural productsCrude peptide mixturesTandem mass spectrometric methodMass spectrometric distinctionMass spectrometric methodRadical ionsIsobaric leucineElectron transferCollisional activationDissociation fragmentationPeptide mixturesNatural productsNominal massSpectrometric methodLiquid chromatographyPeptide sequencingPresence of hydroxyprolineIonsPeptide libraryDifferent peptidesDissociation approachHydroxyproline residuesMass spectrometric identification of an intramolecular disulfide bond in thermally inactivated triosephosphate isomerase from a thermophilic organism Methanocaldococcus jannaschii
Banerjee M, Gupta K, Balaram H, Balaram P. Mass spectrometric identification of an intramolecular disulfide bond in thermally inactivated triosephosphate isomerase from a thermophilic organism Methanocaldococcus jannaschii. Rapid Communications In Mass Spectrometry 2011, 25: 1915-1923. PMID: 21698673, DOI: 10.1002/rcm.5058.Peer-Reviewed Original ResearchConceptsMethanocaldococcus jannaschiiTriosephosphate isomeraseDisulfide bond formationIntramolecular disulfide bondsCysteine thiol groupsMonomer molecular massThree-dimensional structureThermophilic enzymesMass spectrometric identificationMS/MS analysisTetrameric enzymeTertiary structureMolecular massDisulfide bridgesDisulfide-bonded moleculesDisulfide bondsMonomeric massJannaschiiCircular dichroismComplete lossSpectrometric identificationIsomeraseEnzymeTryptic digestDa difference