2014
Local and global analysis of endocytic patch dynamics in fission yeast using a new “temporal superresolution” realignment method
Berro J, Pollard TD. Local and global analysis of endocytic patch dynamics in fission yeast using a new “temporal superresolution” realignment method. Molecular Biology Of The Cell 2014, 25: 3501-3514. PMID: 25143395, PMCID: PMC4230612, DOI: 10.1091/mbc.e13-01-0004.Peer-Reviewed Original ResearchConceptsFission yeastEndocytic actin patchesWild-type cellsEndocytic patchesActin patchesQuantitative microscopyActin assemblyCellular processesVesicle movementEndocytic vesiclesInterphase cellsVesicle formationMolecular mechanismsPatch dynamicsYeastCell lengthGlobal analysisNumber of patchesMicroscopy moviesCellsClathrinEndocytosisNew toolValuable toolPatches
2010
Quantitative Analysis of the Mechanism of Endocytic Actin Patch Assembly and Disassembly in Fission Yeast
Sirotkin V, Berro J, Macmillan K, Zhao L, Pollard TD. Quantitative Analysis of the Mechanism of Endocytic Actin Patch Assembly and Disassembly in Fission Yeast. Molecular Biology Of The Cell 2010, 21: 2894-2904. PMID: 20587778, PMCID: PMC2921122, DOI: 10.1091/mbc.e10-02-0157.Peer-Reviewed Original ResearchConceptsFission yeastActin assemblyActin patch assemblyEndocytic actin patchesEndocytic adaptor proteinActivators of Arp2/3Plasma membrane invaginationsRecruitment of dynaminQuantitative confocal microscopyActin filament fragmentationActin patchesArp2/3 complexPatch assemblyAdaptor proteinMembrane invaginationsCross-linked filamentsFluorescent proteinClathrin moleculesFilament fragmentationProteinConfocal microscopyYeastBranched networkIndividual patchesAssembly