2023
Cab45 deficiency leads to the mistargeting of progranulin and prosaposin and aberrant lysosomal positioning
Tran M, Tüshaus J, Kim Y, Ramazanov B, Devireddy S, Lichtenthaler S, Ferguson S, von Blume J. Cab45 deficiency leads to the mistargeting of progranulin and prosaposin and aberrant lysosomal positioning. Traffic 2023, 24: 4-19. PMID: 36398980, PMCID: PMC9825660, DOI: 10.1111/tra.12873.Peer-Reviewed Original Research
2022
Liquid–liquid phase separation facilitates the biogenesis of secretory storage granules
Parchure A, Tian M, Stalder D, Boyer C, Bearrows S, Rohli K, Zhang J, Rivera-Molina F, Ramazanov B, Mahata S, Wang Y, Stephens S, Gershlick D, von Blume J. Liquid–liquid phase separation facilitates the biogenesis of secretory storage granules. Journal Of Cell Biology 2022, 221: e202206132. PMID: 36173346, PMCID: PMC9526250, DOI: 10.1083/jcb.202206132.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkLiquid-liquid phase separationSecretory granulesClient moleculesSecretory storage granulesΒ-cellsMembrane traffickingCargo receptorsPlasma membranePancreatic β-cellsProcessing enzymesCell typesStorage granulesChromogranin proteinsBiogenesisAcidic pHTraffickingGranulesProteinEnzyme
2021
Cargo sorting at the trans-Golgi network at a glance
Ford C, Parchure A, von Blume J, Burd CG. Cargo sorting at the trans-Golgi network at a glance. Journal Of Cell Science 2021, 134 PMID: 34870705, PMCID: PMC8714066, DOI: 10.1242/jcs.259110.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkDistinct export pathwaysClathrin-coated vesiclesTrafficking of glycoproteinsMechanism of proteinEndo-lysosomal organellesAccompanying posterGlance articleResident proteinsExport pathwayGolgi functionTrans-GolgiDifferent organellesGolgi cisternaeKey molecular featuresTrans cisternaeMembrane sacsFinal compartmentGolgiCell surfaceDistinct repertoireCell scienceTransport carriersProteinOrganelles
2020
Nucleobindin-1 regulates ECM degradation by promoting intra-Golgi trafficking of MMPs
Pacheco-Fernandez N, Pakdel M, Blank B, Sanchez-Gonzalez I, Weber K, Tran ML, Hecht TK, Gautsch R, Beck G, Perez F, Hausser A, Linder S, von Blume J. Nucleobindin-1 regulates ECM degradation by promoting intra-Golgi trafficking of MMPs. Journal Of Cell Biology 2020, 219: e201907058. PMID: 32479594, PMCID: PMC7401813, DOI: 10.1083/jcb.201907058.Peer-Reviewed Original ResearchFam20C regulates protein secretion by Cab45 phosphorylation
Hecht TK, Blank B, Steger M, Lopez V, Beck G, Ramazanov B, Mann M, Tagliabracci V, von Blume J. Fam20C regulates protein secretion by Cab45 phosphorylation. Journal Of Cell Biology 2020, 219: e201910089. PMID: 32422653, PMCID: PMC7265331, DOI: 10.1083/jcb.201910089.Peer-Reviewed Original Research
2019
Lipid‐dependent coupling of secretory cargo sorting and trafficking at the trans‐Golgi network
von Blume J, Hausser A. Lipid‐dependent coupling of secretory cargo sorting and trafficking at the trans‐Golgi network. FEBS Letters 2019, 593: 2412-2427. PMID: 31344259, PMCID: PMC8048779, DOI: 10.1002/1873-3468.13552.Peer-Reviewed Original ResearchSignal peptide peptidase‐like 2c impairs vesicular transport and cleaves SNARE proteins
Papadopoulou A, Müller S, Mentrup T, Shmueli M, Niemeyer J, Haug‐Kröper M, von Blume J, Mayerhofer A, Feederle R, Schröder B, Lichtenthaler S, Fluhrer R. Signal peptide peptidase‐like 2c impairs vesicular transport and cleaves SNARE proteins. EMBO Reports 2019, 20: embr201846451. PMID: 30733281, PMCID: PMC6399617, DOI: 10.15252/embr.201846451.Peer-Reviewed Original ResearchMeSH KeywordsAcrosomeAnimalsAspartic Acid EndopeptidasesBiocatalysisDown-RegulationGlycomicsGlycoproteinsGlycosyltransferasesGolgi ApparatusHEK293 CellsHumansMaleMembrane ProteinsMice, Inbred C57BLModels, BiologicalProtein TransportProteolysisSNARE ProteinsSpermatidsSubcellular FractionsSubstrate SpecificityConceptsSNARE proteinsCandidate substratesVesicular transportProcess of vesicular traffickingImpaired vesicular transportIntramembrane aspartyl proteaseCleave SNARE proteinsGxGD-typeAcrosome formationB cell developmentCargo proteinsVesicular traffickingAspartyl proteaseProtease familyCellular processesProtein glycosylationSubcellular compartmentsEndoplasmic reticulumProteolytic processingBiological processesAlzheimer's diseasePhysiological functionsProteinDevelopment of pathologyProtease
2018
Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network
Deng Y, Pakdel M, Blank B, Sundberg EL, Burd CG, von Blume J. Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network. Developmental Cell 2018, 47: 464-478.e8. PMID: 30393074, PMCID: PMC6261503, DOI: 10.1016/j.devcel.2018.10.012.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkTGN membranesCell surface transportProtein sortingTrafficking pathwaysSecretion pathwaySecretory proteinsSphingomyelin synthesisSorting mechanismLipid synthesisProteinVesicular carriersCab45Sphingomyelin contentNew lipidSortingPrincipal functionMembranePathwayCore componentLipidsGolgiSurface transportExportActivityExploring new routes for secretory protein export from the trans-Golgi network
Pakdel M, von Blume J. Exploring new routes for secretory protein export from the trans-Golgi network. Molecular Biology Of The Cell 2018, 29: 235-240. PMID: 29382805, PMCID: PMC5996961, DOI: 10.1091/mbc.e17-02-0117.Peer-Reviewed Original ResearchConceptsSecretory cargoSecretory proteinsCytoplasmic actin cytoskeletonMajor sorting stationTrans-Golgi networkGolgi residentsProtein exportGolgi networkActin cytoskeletonATPase SPCA1Cellular compartmentsTissue homeostasisSorting stationSoluble proteinTGNLysosomal hydrolasesOverall pathwayLuminal CaRecent discoveryProteinCargoSpecific carrierSortingCellsFinal destination
2017
Persistent inhibition of pore-based cell migration by sub-toxic doses of miuraenamide, an actin filament stabilizer
Moser C, Rüdiger D, Förster F, von Blume J, Yu P, Kuster B, Kazmaier U, Vollmar AM, Zahler S. Persistent inhibition of pore-based cell migration by sub-toxic doses of miuraenamide, an actin filament stabilizer. Scientific Reports 2017, 7: 16407. PMID: 29180826, PMCID: PMC5703899, DOI: 10.1038/s41598-017-16759-7.Peer-Reviewed Original ResearchConceptsCell migrationActin-targeting drugsActin-binding compoundsSub-toxic dosesTumor cell migrationProteomic approachSRF signalingNuclear stiffnessActin filament stabilizerProtein clustersActin filamentsH treatmentComplex biologyProtein expressionActinPersistent morphological changesSmall moleculesMiuraenamidesGeneral cytotoxicityNatural compoundsCytotoxic effectsRegulationMorphological changesBoyden chamberMigrationCab45—Unraveling key features of a novel secretory cargo sorter at the trans-Golgi network
Blank B, von Blume J. Cab45—Unraveling key features of a novel secretory cargo sorter at the trans-Golgi network. European Journal Of Cell Biology 2017, 96: 383-390. PMID: 28372832, DOI: 10.1016/j.ejcb.2017.03.001.Peer-Reviewed Original ResearchConceptsMolecular functionsCargo moleculesCytoplasmic actin cytoskeletonSpecific cargo moleculesTransmembrane cargo receptorsTrans-Golgi networkMannose-6-phosphate receptorCargo receptorsGolgi networkActin cytoskeletonATPase SPCA1Secreted proteinsPlasma membraneSecretory proteinsExtracellular proteasesCab45Sorting mechanismSurface receptorsProteinExtracellular spaceSpecific carrierPossible roleFinal destinationSortingReceptors
2016
Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network
Crevenna AH, Blank B, Maiser A, Emin D, Prescher J, Beck G, Kienzle C, Bartnik K, Habermann B, Pakdel M, Leonhardt H, Lamb DC, von Blume J. Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network. Journal Of Cell Biology 2016, 213: 305-314. PMID: 27138253, PMCID: PMC4862333, DOI: 10.1083/jcb.201601089.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSecretory cargoSecretory proteinsSpecific cargo moleculesTransmembrane cargoCargo moleculesSuperresolution microscopyIntact cellsCab45Lysosomal hydrolasesExtracellular spaceDependent mannerCargoProteinOligomerizationSortingHydrolasesDependent changesMicrodomainsMutationsOligomersElucidationCellsCa2
2015
Julia von Blume: Sorting through the trans-Golgi
von Blume J, Sedwick C. Julia von Blume: Sorting through the trans-Golgi. Journal Of Cell Biology 2015, 208: 4-5. PMID: 25559180, PMCID: PMC4284231, DOI: 10.1083/jcb.2081pi.Peer-Reviewed Original Research
2014
JAGN1 deficiency causes aberrant myeloid cell homeostasis and congenital neutropenia
Boztug K, Järvinen PM, Salzer E, Racek T, Mönch S, Garncarz W, Gertz EM, Schäffer AA, Antonopoulos A, Haslam SM, Schieck L, Puchałka J, Diestelhorst J, Appaswamy G, Lescoeur B, Giambruno R, Bigenzahn JW, Elling U, Pfeifer D, Conde CD, Albert MH, Welte K, Brandes G, Sherkat R, van der Werff ten Bosch J, Rezaei N, Etzioni A, Bellanné-Chantelot C, Superti-Furga G, Penninger JM, Bennett KL, von Blume J, Dell A, Donadieu J, Klein C. JAGN1 deficiency causes aberrant myeloid cell homeostasis and congenital neutropenia. Nature Genetics 2014, 46: 1021-1027. PMID: 25129144, PMCID: PMC4829076, DOI: 10.1038/ng.3069.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultApoptosisCell DifferentiationCell SurvivalChildChild, PreschoolCongenital Bone Marrow Failure SyndromesFemaleGlycosylationHomeostasisHumansInfantInfant, NewbornMaleMembrane ProteinsMutationMyeloid CellsNeutropeniaNeutrophilsReceptors, Granulocyte Colony-Stimulating FactorSignal TransductionYoung AdultCofilin recruits F-actin to SPCA1 and promotes Ca2+-mediated secretory cargo sorting
Kienzle C, Basnet N, Crevenna AH, Beck G, Habermann B, Mizuno N, von Blume J. Cofilin recruits F-actin to SPCA1 and promotes Ca2+-mediated secretory cargo sorting. Journal Of Cell Biology 2014, 206: 635-654. PMID: 25179631, PMCID: PMC4151145, DOI: 10.1083/jcb.201311052.Peer-Reviewed Original ResearchSecretory cargo sorting at the trans-Golgi network
Kienzle C, von Blume J. Secretory cargo sorting at the trans-Golgi network. Trends In Cell Biology 2014, 24: 584-593. PMID: 24841758, DOI: 10.1016/j.tcb.2014.04.007.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkCytoplasmic actin cytoskeletonPlasma membrane deliverySubset of proteinsNovel sorting mechanismKey cellular eventsSecretory cargoActin cytoskeletonCellular eventsBinding proteinLysosomal hydrolasesSorting mechanismProteinNormal physiologySuccessful sortingRecent discoverySortingCab45CytoskeletonHydrolasesSPCA1ATPaseRegulationPhysiologyCargo
2013
Directing Traffic into the Future
Antonny B, Audhya J, l Bagnat M, von Blume J, Briggs JA, Giraudo C, Kaeser PS, Miller E, Reinisch K, Sbalzarini IF, Schuldiner M, Shen J, Takamori S, Verstreken P, Walther T. Directing Traffic into the Future. Developmental Cell 2013, 27: 480-484. PMID: 24482802, DOI: 10.1016/j.devcel.2013.11.017.Peer-Reviewed Original Research
2012
Cab45 is required for Ca2+-dependent secretory cargo sorting at the trans-Golgi network
von Blume J, Alleaume AM, Kienzle C, Carreras-Sureda A, Valverde M, Malhotra V. Cab45 is required for Ca2+-dependent secretory cargo sorting at the trans-Golgi network. Journal Of Cell Biology 2012, 199: 1057-1066. PMID: 23266954, PMCID: PMC3529532, DOI: 10.1083/jcb.201207180.Peer-Reviewed Original ResearchMeSH KeywordsCalciumCalcium SignalingCalcium-Binding ProteinsCartilage Oligomeric Matrix ProteinCathepsin DExtracellular Matrix ProteinsGene Knockdown TechniquesGlycoproteinsHeLa CellsHomeostasisHumansMatrilin ProteinsProtein BindingProtein IsoformsProtein TransportRNA InterferenceSecretory PathwayTrans-Golgi Network
2011
ADF/Cofilin Regulates Secretory Cargo Sorting at the TGN via the Ca2+ ATPase SPCA1
von Blume J, Alleaume AM, Cantero-Recasens G, Curwin A, Carreras-Sureda A, Zimmermann T, van Galen J, Wakana Y, Valverde MA, Malhotra V. ADF/Cofilin Regulates Secretory Cargo Sorting at the TGN via the Ca2+ ATPase SPCA1. Developmental Cell 2011, 20: 652-662. PMID: 21571222, DOI: 10.1016/j.devcel.2011.03.014.Peer-Reviewed Original Research
2010
Role of the Second Cysteine-rich Domain and Pro275 in Protein Kinase D2 Interaction with ADP-Ribosylation Factor 1, Trans-Golgi Network Recruitment, and Protein Transport
Pusapati GV, Krndija D, Armacki M, von Wichert G, von Blume J, Malhotra V, Adler G, Seufferlein T. Role of the Second Cysteine-rich Domain and Pro275 in Protein Kinase D2 Interaction with ADP-Ribosylation Factor 1, Trans-Golgi Network Recruitment, and Protein Transport. Molecular Biology Of The Cell 2010, 21: 1011-1022. PMID: 20089835, PMCID: PMC2836954, DOI: 10.1091/mbc.e09-09-0814.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSecond cysteine-rich domainCysteine-rich domainProtein transportC1a domainPlasma membraneADP-ribosylation factor 1ADP-ribosylation factorPKD isoenzymesTGN membranesSecretory trafficPKD2 functionsSmall GTPaseC1b domainKey regulatorPKD2Transport carriersARF1Factor 1PKDPrecise mechanismMembraneRecruitmentDomainArf6