2020
Nucleobindin-1 regulates ECM degradation by promoting intra-Golgi trafficking of MMPs
Pacheco-Fernandez N, Pakdel M, Blank B, Sanchez-Gonzalez I, Weber K, Tran ML, Hecht TK, Gautsch R, Beck G, Perez F, Hausser A, Linder S, von Blume J. Nucleobindin-1 regulates ECM degradation by promoting intra-Golgi trafficking of MMPs. Journal Of Cell Biology 2020, 219: e201907058. PMID: 32479594, PMCID: PMC7401813, DOI: 10.1083/jcb.201907058.Peer-Reviewed Original Research
2018
Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network
Deng Y, Pakdel M, Blank B, Sundberg EL, Burd CG, von Blume J. Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network. Developmental Cell 2018, 47: 464-478.e8. PMID: 30393074, PMCID: PMC6261503, DOI: 10.1016/j.devcel.2018.10.012.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkTGN membranesCell surface transportProtein sortingTrafficking pathwaysSecretion pathwaySecretory proteinsSphingomyelin synthesisSorting mechanismLipid synthesisProteinVesicular carriersCab45Sphingomyelin contentNew lipidSortingPrincipal functionMembranePathwayCore componentLipidsGolgiSurface transportExportActivityExploring new routes for secretory protein export from the trans-Golgi network
Pakdel M, von Blume J. Exploring new routes for secretory protein export from the trans-Golgi network. Molecular Biology Of The Cell 2018, 29: 235-240. PMID: 29382805, PMCID: PMC5996961, DOI: 10.1091/mbc.e17-02-0117.Peer-Reviewed Original ResearchConceptsSecretory cargoSecretory proteinsCytoplasmic actin cytoskeletonMajor sorting stationTrans-Golgi networkGolgi residentsProtein exportGolgi networkActin cytoskeletonATPase SPCA1Cellular compartmentsTissue homeostasisSorting stationSoluble proteinTGNLysosomal hydrolasesOverall pathwayLuminal CaRecent discoveryProteinCargoSpecific carrierSortingCellsFinal destination
2016
Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network
Crevenna AH, Blank B, Maiser A, Emin D, Prescher J, Beck G, Kienzle C, Bartnik K, Habermann B, Pakdel M, Leonhardt H, Lamb DC, von Blume J. Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network. Journal Of Cell Biology 2016, 213: 305-314. PMID: 27138253, PMCID: PMC4862333, DOI: 10.1083/jcb.201601089.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSecretory cargoSecretory proteinsSpecific cargo moleculesTransmembrane cargoCargo moleculesSuperresolution microscopyIntact cellsCab45Lysosomal hydrolasesExtracellular spaceDependent mannerCargoProteinOligomerizationSortingHydrolasesDependent changesMicrodomainsMutationsOligomersElucidationCellsCa2
2014
Cofilin recruits F-actin to SPCA1 and promotes Ca2+-mediated secretory cargo sorting
Kienzle C, Basnet N, Crevenna AH, Beck G, Habermann B, Mizuno N, von Blume J. Cofilin recruits F-actin to SPCA1 and promotes Ca2+-mediated secretory cargo sorting. Journal Of Cell Biology 2014, 206: 635-654. PMID: 25179631, PMCID: PMC4151145, DOI: 10.1083/jcb.201311052.Peer-Reviewed Original Research
2012
Cab45 is required for Ca2+-dependent secretory cargo sorting at the trans-Golgi network
von Blume J, Alleaume AM, Kienzle C, Carreras-Sureda A, Valverde M, Malhotra V. Cab45 is required for Ca2+-dependent secretory cargo sorting at the trans-Golgi network. Journal Of Cell Biology 2012, 199: 1057-1066. PMID: 23266954, PMCID: PMC3529532, DOI: 10.1083/jcb.201207180.Peer-Reviewed Original ResearchMeSH KeywordsCalciumCalcium SignalingCalcium-Binding ProteinsCartilage Oligomeric Matrix ProteinCathepsin DExtracellular Matrix ProteinsGene Knockdown TechniquesGlycoproteinsHeLa CellsHomeostasisHumansMatrilin ProteinsProtein BindingProtein IsoformsProtein TransportRNA InterferenceSecretory PathwayTrans-Golgi Network
2011
ADF/Cofilin Regulates Secretory Cargo Sorting at the TGN via the Ca2+ ATPase SPCA1
von Blume J, Alleaume AM, Cantero-Recasens G, Curwin A, Carreras-Sureda A, Zimmermann T, van Galen J, Wakana Y, Valverde MA, Malhotra V. ADF/Cofilin Regulates Secretory Cargo Sorting at the TGN via the Ca2+ ATPase SPCA1. Developmental Cell 2011, 20: 652-662. PMID: 21571222, DOI: 10.1016/j.devcel.2011.03.014.Peer-Reviewed Original Research
2009
Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network
von Blume J, Duran JM, Forlanelli E, Alleaume AM, Egorov M, Polishchuk R, Molina H, Malhotra V. Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network. Journal Of Cell Biology 2009, 187: 1055-1069. PMID: 20026655, PMCID: PMC2806282, DOI: 10.1083/jcb.200908040.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkADF/cofilinActin-depolymerizing factorSecretory proteinsSecretory cargoCofilin knockdownMass spectrometry-based protein profilingMammalian tissue culture cellsGolgi-resident proteinsSorting of proteinsEndogenous secretory proteinsIntegral membrane proteinsSoluble secretory proteinsStable isotope labelingTissue culture cellsDrosophila melanogasterActin remodelingMammalian cellsMembrane proteinsGolgi membranesProtein profilingIsotope labelingCofilinCell surfaceAmino acids
2007
Phosphorylation at Ser244 by CK1 determines nuclear localization and substrate targeting of PKD2
von Blume J, Knippschild U, Dequiedt F, Giamas G, Beck A, Auer A, Van Lint J, Adler G, Seufferlein T. Phosphorylation at Ser244 by CK1 determines nuclear localization and substrate targeting of PKD2. The EMBO Journal 2007, 26: 4619-4633. PMID: 17962809, PMCID: PMC2080801, DOI: 10.1038/sj.emboj.7601891.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCasein Kinase 1 epsilonCasein Kinase IdeltaCell LineCell Line, TumorCell NucleusChlorocebus aethiopsCOS CellsDNA-Binding ProteinsGastrinsHeLa CellsHistone DeacetylasesHumansNuclear Receptor Subfamily 4, Group A, Member 1PhosphorylationProtein Kinase CProtein Kinase D2Protein KinasesReceptor, Cholecystokinin BReceptors, Cytoplasmic and NuclearReceptors, SteroidSerineTranscription FactorsConceptsProtein kinase D2Nuclear accumulationEfficient phosphorylationCritical post-translational modificationSerine/threonine kinaseZinc finger domainPost-translational modificationsSignal transduction pathwaysG protein-coupled receptorsHuman gastric cancer cellsExport machinerySubstrate targetingHDAC7 phosphorylationActivation loopNuclear substratesThreonine kinaseActive kinaseNuclear exportGastric cancer cellsSubcellular compartmentsTransduction pathwaysNuclear localizationPKD familyPKC etaPhosphorylation
2006
New Role for hPar-1 Kinases EMK and C-TAK1 in Regulating Localization and Activity of Class IIa Histone Deacetylases
Dequiedt F, Martin M, Von Blume J, Vertommen D, Lecomte E, Mari N, Heinen MF, Bachmann M, Twizere JC, Huang MC, Rider MH, Piwnica-Worms H, Seufferlein T, Kettmann R. New Role for hPar-1 Kinases EMK and C-TAK1 in Regulating Localization and Activity of Class IIa Histone Deacetylases. Molecular And Cellular Biology 2006, 26: 7086-7102. PMID: 16980613, PMCID: PMC1592903, DOI: 10.1128/mcb.00231-06.Peer-Reviewed Original ResearchMeSH Keywords14-3-3 ProteinsActive Transport, Cell NucleusAmino Acid SequenceAnimalsBinding SitesCell NucleusCells, CulturedChlorocebus aethiopsCOS CellsCytoplasmHeLa CellsHistone DeacetylasesHumansMolecular Sequence DataPhosphorylationPhosphoserineProtein Serine-Threonine KinasesProtein TransportSubstrate SpecificityConceptsMicrotubule affinity-regulating kinaseClass IIa HDACsN-terminal serine residueClass IIa histoneIIa HDACsC-TAK1IIa histoneSerine residuesMajor developmental programsMARK/ParVariety of kinasesHierarchical phosphorylationRepressive activityNuclear exportRepressive functionDevelopmental programSubcellular localizationNuclear exclusionConstitutive phosphorylationSophisticated regulationPhosphorylationKinaseSpecific signalsHDACsHistones