2024
Calcium flow at ER-TGN contact sites facilitates secretory cargo export
Ramazanov B, Parchure A, Di Martino R, Kumar A, Chung M, Kim Y, Griesbeck O, Schwartz M, Luini A, von Blume J. Calcium flow at ER-TGN contact sites facilitates secretory cargo export. Molecular Biology Of The Cell 2024, 35: ar50. PMID: 38294859, PMCID: PMC11064664, DOI: 10.1091/mbc.e23-03-0099.Peer-Reviewed Original ResearchMeSH KeywordsBiological TransportCalciumCarrier ProteinsEndoplasmic ReticulumProtein TransportProteinsTrans-Golgi Network
2023
Sorting secretory proteins
Parchure A, von Blume J. Sorting secretory proteins. ELife 2023, 12: e93490. PMID: 37997893, PMCID: PMC10672786, DOI: 10.7554/elife.93490.Peer-Reviewed Original ResearchRediscovering the intricacies of secretory granule biogenesis
Campelo F, Tian M, von Blume J. Rediscovering the intricacies of secretory granule biogenesis. Current Opinion In Cell Biology 2023, 85: 102231. PMID: 37657367, DOI: 10.1016/j.ceb.2023.102231.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSG biogenesisRegulated secretionImmature SGsEssential cellular processesSecretory granulesSecretory granule biogenesisImmature secretory granulesCargo selectionCargo receptorsCellular processesGranule biogenesisGranule cargoCargo moleculesBiogenesisBiophysical mechanismsDisease mechanismsCellular communicationCargoCentral roleDiverse rangeNew lightTraffickingMechanismProteinCab45 deficiency leads to the mistargeting of progranulin and prosaposin and aberrant lysosomal positioning
Tran M, Tüshaus J, Kim Y, Ramazanov B, Devireddy S, Lichtenthaler S, Ferguson S, von Blume J. Cab45 deficiency leads to the mistargeting of progranulin and prosaposin and aberrant lysosomal positioning. Traffic 2023, 24: 4-19. PMID: 36398980, PMCID: PMC9825660, DOI: 10.1111/tra.12873.Peer-Reviewed Original Research
2022
Quantification of Protein Exit at the Trans-Golgi Network
Tran M, Kim Y, von Blume J. Quantification of Protein Exit at the Trans-Golgi Network. Methods In Molecular Biology 2022, 2557: 583-594. PMID: 36512239, DOI: 10.1007/978-1-0716-2639-9_35.Peer-Reviewed Original ResearchMeSH KeywordsGolgi ApparatusHomeostasisProtein TransportProteinsSecretory PathwayTrans-Golgi NetworkConceptsTrans-Golgi networkProtein exportSecretory pathwaySelective hooks (RUSH) systemCell biological approachesProtein of interestPulse-chase experimentsProtein exitProtein sortingExport kineticsCellular homeostasisProtein transportDifferent cargoesLive cellsBiological approachesRUSH systemProteinPathwayTraffickingHomeostasisExportCargoSortingCompartmentsCells
2021
Cargo sorting at the trans-Golgi network at a glance
Ford C, Parchure A, von Blume J, Burd CG. Cargo sorting at the trans-Golgi network at a glance. Journal Of Cell Science 2021, 134 PMID: 34870705, PMCID: PMC8714066, DOI: 10.1242/jcs.259110.Peer-Reviewed Original ResearchMeSH KeywordsCell MembraneClathrin-Coated VesiclesGolgi ApparatusHumansProtein TransportTrans-Golgi NetworkConceptsTrans-Golgi networkDistinct export pathwaysClathrin-coated vesiclesTrafficking of glycoproteinsMechanism of proteinEndo-lysosomal organellesAccompanying posterGlance articleResident proteinsExport pathwayGolgi functionTrans-GolgiDifferent organellesGolgi cisternaeKey molecular featuresTrans cisternaeMembrane sacsFinal compartmentGolgiCell surfaceDistinct repertoireCell scienceTransport carriersProteinOrganellesSending out molecules from the TGN
Ramazanov BR, Tran ML, von Blume J. Sending out molecules from the TGN. Current Opinion In Cell Biology 2021, 71: 55-62. PMID: 33706234, PMCID: PMC8328904, DOI: 10.1016/j.ceb.2021.02.005.Peer-Reviewed Original ResearchMeSH KeywordsCell MembraneEndoplasmic ReticulumMembrane ProteinsProtein TransportTrans-Golgi NetworkConceptsTrans-Golgi networkEndoplasmic reticulum contact sitesCell-cell communicationExtracellular matrix proteinsMembrane traffickingCargo proteinsCargo sortingTGN structureTransmembrane proteinPlasma membraneContact sitesExciting linkSoluble proteinMatrix proteinsSorting mechanismProteinTransport carriersMajor open questionsSortingMembraneNovel typeTraffickingMachineryOrganismsVesicles
2020
Fam20C regulates protein secretion by Cab45 phosphorylation
Hecht TK, Blank B, Steger M, Lopez V, Beck G, Ramazanov B, Mann M, Tagliabracci V, von Blume J. Fam20C regulates protein secretion by Cab45 phosphorylation. Journal Of Cell Biology 2020, 219: e201910089. PMID: 32422653, PMCID: PMC7265331, DOI: 10.1083/jcb.201910089.Peer-Reviewed Original Research
2019
Lipid‐dependent coupling of secretory cargo sorting and trafficking at the trans‐Golgi network
von Blume J, Hausser A. Lipid‐dependent coupling of secretory cargo sorting and trafficking at the trans‐Golgi network. FEBS Letters 2019, 593: 2412-2427. PMID: 31344259, PMCID: PMC8048779, DOI: 10.1002/1873-3468.13552.Peer-Reviewed Original Research
2018
Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network
Deng Y, Pakdel M, Blank B, Sundberg EL, Burd CG, von Blume J. Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network. Developmental Cell 2018, 47: 464-478.e8. PMID: 30393074, PMCID: PMC6261503, DOI: 10.1016/j.devcel.2018.10.012.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkTGN membranesCell surface transportProtein sortingTrafficking pathwaysSecretion pathwaySecretory proteinsSphingomyelin synthesisSorting mechanismLipid synthesisProteinVesicular carriersCab45Sphingomyelin contentNew lipidSortingPrincipal functionMembranePathwayCore componentLipidsGolgiSurface transportExportActivityExploring new routes for secretory protein export from the trans-Golgi network
Pakdel M, von Blume J. Exploring new routes for secretory protein export from the trans-Golgi network. Molecular Biology Of The Cell 2018, 29: 235-240. PMID: 29382805, PMCID: PMC5996961, DOI: 10.1091/mbc.e17-02-0117.Peer-Reviewed Original ResearchConceptsSecretory cargoSecretory proteinsCytoplasmic actin cytoskeletonMajor sorting stationTrans-Golgi networkGolgi residentsProtein exportGolgi networkActin cytoskeletonATPase SPCA1Cellular compartmentsTissue homeostasisSorting stationSoluble proteinTGNLysosomal hydrolasesOverall pathwayLuminal CaRecent discoveryProteinCargoSpecific carrierSortingCellsFinal destination
2017
Cab45—Unraveling key features of a novel secretory cargo sorter at the trans-Golgi network
Blank B, von Blume J. Cab45—Unraveling key features of a novel secretory cargo sorter at the trans-Golgi network. European Journal Of Cell Biology 2017, 96: 383-390. PMID: 28372832, DOI: 10.1016/j.ejcb.2017.03.001.Peer-Reviewed Original ResearchConceptsMolecular functionsCargo moleculesCytoplasmic actin cytoskeletonSpecific cargo moleculesTransmembrane cargo receptorsTrans-Golgi networkMannose-6-phosphate receptorCargo receptorsGolgi networkActin cytoskeletonATPase SPCA1Secreted proteinsPlasma membraneSecretory proteinsExtracellular proteasesCab45Sorting mechanismSurface receptorsProteinExtracellular spaceSpecific carrierPossible roleFinal destinationSortingReceptors
2016
Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network
Crevenna AH, Blank B, Maiser A, Emin D, Prescher J, Beck G, Kienzle C, Bartnik K, Habermann B, Pakdel M, Leonhardt H, Lamb DC, von Blume J. Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network. Journal Of Cell Biology 2016, 213: 305-314. PMID: 27138253, PMCID: PMC4862333, DOI: 10.1083/jcb.201601089.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSecretory cargoSecretory proteinsSpecific cargo moleculesTransmembrane cargoCargo moleculesSuperresolution microscopyIntact cellsCab45Lysosomal hydrolasesExtracellular spaceDependent mannerCargoProteinOligomerizationSortingHydrolasesDependent changesMicrodomainsMutationsOligomersElucidationCellsCa2
2015
Julia von Blume: Sorting through the trans-Golgi
von Blume J, Sedwick C. Julia von Blume: Sorting through the trans-Golgi. Journal Of Cell Biology 2015, 208: 4-5. PMID: 25559180, PMCID: PMC4284231, DOI: 10.1083/jcb.2081pi.Peer-Reviewed Original Research
2014
Secretory cargo sorting at the trans-Golgi network
Kienzle C, von Blume J. Secretory cargo sorting at the trans-Golgi network. Trends In Cell Biology 2014, 24: 584-593. PMID: 24841758, DOI: 10.1016/j.tcb.2014.04.007.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonAnimalsBiological TransportCell MembraneHumansIntracellular MembranesProtein TransportSecretory PathwayTrans-Golgi NetworkConceptsTrans-Golgi networkCytoplasmic actin cytoskeletonPlasma membrane deliverySubset of proteinsNovel sorting mechanismKey cellular eventsSecretory cargoActin cytoskeletonCellular eventsBinding proteinLysosomal hydrolasesSorting mechanismProteinNormal physiologySuccessful sortingRecent discoverySortingCab45CytoskeletonHydrolasesSPCA1ATPaseRegulationPhysiologyCargo
2012
Cab45 is required for Ca2+-dependent secretory cargo sorting at the trans-Golgi network
von Blume J, Alleaume AM, Kienzle C, Carreras-Sureda A, Valverde M, Malhotra V. Cab45 is required for Ca2+-dependent secretory cargo sorting at the trans-Golgi network. Journal Of Cell Biology 2012, 199: 1057-1066. PMID: 23266954, PMCID: PMC3529532, DOI: 10.1083/jcb.201207180.Peer-Reviewed Original ResearchMeSH KeywordsCalciumCalcium SignalingCalcium-Binding ProteinsCartilage Oligomeric Matrix ProteinCathepsin DExtracellular Matrix ProteinsGene Knockdown TechniquesGlycoproteinsHeLa CellsHomeostasisHumansMatrilin ProteinsProtein BindingProtein IsoformsProtein TransportRNA InterferenceSecretory PathwayTrans-Golgi NetworkCofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast
Curwin AJ, von Blume J, Malhotra V. Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast. Molecular Biology Of The Cell 2012, 23: 2327-2338. PMID: 22553351, PMCID: PMC3374751, DOI: 10.1091/mbc.e11-09-0826.Peer-Reviewed Original ResearchMeSH KeywordsActin Depolymerizing FactorsBlotting, WesternCathepsin ACell MembraneCofilin 1EndocytosisGlucan Endo-1,3-beta-D-GlucosidaseGreen Fluorescent ProteinsIntracellular MembranesMicroscopy, FluorescenceMutationProtein TransportProton-Translocating ATPasesR-SNARE ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTrans-Golgi NetworkVacuolesConceptsTrans-Golgi networkLate Golgi membranesCarboxypeptidase YSecretory cargoGolgi membranesLate Golgi compartmentMechanisms of cargoSoluble secretory proteinsRole of cofilinATPase Pma1Yeast orthologueSynthetic sicknessProtein cofilinSpecific cargoMutant cellsMammalian cellsGolgi compartmentSorting defectsPlasma membraneSecretory proteinsGolgi apparatusATPase 1CofilinCell surfacePMR1
2011
ADF/Cofilin Regulates Secretory Cargo Sorting at the TGN via the Ca2+ ATPase SPCA1
von Blume J, Alleaume AM, Cantero-Recasens G, Curwin A, Carreras-Sureda A, Zimmermann T, van Galen J, Wakana Y, Valverde MA, Malhotra V. ADF/Cofilin Regulates Secretory Cargo Sorting at the TGN via the Ca2+ ATPase SPCA1. Developmental Cell 2011, 20: 652-662. PMID: 21571222, DOI: 10.1016/j.devcel.2011.03.014.Peer-Reviewed Original ResearchMeSH KeywordsActin Depolymerizing FactorsCalciumCalcium-Transporting ATPasesCells, CulturedDestrinEndoplasmic ReticulumHeLa CellsHumansTrans-Golgi Network
2010
Role of the Second Cysteine-rich Domain and Pro275 in Protein Kinase D2 Interaction with ADP-Ribosylation Factor 1, Trans-Golgi Network Recruitment, and Protein Transport
Pusapati GV, Krndija D, Armacki M, von Wichert G, von Blume J, Malhotra V, Adler G, Seufferlein T. Role of the Second Cysteine-rich Domain and Pro275 in Protein Kinase D2 Interaction with ADP-Ribosylation Factor 1, Trans-Golgi Network Recruitment, and Protein Transport. Molecular Biology Of The Cell 2010, 21: 1011-1022. PMID: 20089835, PMCID: PMC2836954, DOI: 10.1091/mbc.e09-09-0814.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSecond cysteine-rich domainCysteine-rich domainProtein transportC1a domainPlasma membraneADP-ribosylation factor 1ADP-ribosylation factorPKD isoenzymesTGN membranesSecretory trafficPKD2 functionsSmall GTPaseC1b domainKey regulatorPKD2Transport carriersARF1Factor 1PKDPrecise mechanismMembraneRecruitmentDomainArf6
2009
Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network
von Blume J, Duran JM, Forlanelli E, Alleaume AM, Egorov M, Polishchuk R, Molina H, Malhotra V. Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network. Journal Of Cell Biology 2009, 187: 1055-1069. PMID: 20026655, PMCID: PMC2806282, DOI: 10.1083/jcb.200908040.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkADF/cofilinActin-depolymerizing factorSecretory proteinsSecretory cargoCofilin knockdownMass spectrometry-based protein profilingMammalian tissue culture cellsGolgi-resident proteinsSorting of proteinsEndogenous secretory proteinsIntegral membrane proteinsSoluble secretory proteinsStable isotope labelingTissue culture cellsDrosophila melanogasterActin remodelingMammalian cellsMembrane proteinsGolgi membranesProtein profilingIsotope labelingCofilinCell surfaceAmino acids