2024
Protein condensates in the the secretory pathway: Unraveling biophysical interactions and function
Campelo F, Lillo J, von Blume J. Protein condensates in the the secretory pathway: Unraveling biophysical interactions and function. Biophysical Journal 2024, 123: 1531-1541. PMID: 38698644, PMCID: PMC11214006, DOI: 10.1016/j.bpj.2024.04.031.Peer-Reviewed Original ResearchSecretory pathwayProtein condensatesGolgi apparatusCellular processesEndoplasmic reticulumMembraneless organellesBiomolecular condensatesCellular consequencesCellular organizationBiochemical reactionsFunctional significanceProteinMembrane boundariesBiophysical interactionsPathwayMembraneSpecific componentsGolgiOrganellesReticulumOrganizationSecretoryCondensation interactionInteractionPhase separation phenomenon
2022
Quantification of Protein Exit at the Trans-Golgi Network
Tran M, Kim Y, von Blume J. Quantification of Protein Exit at the Trans-Golgi Network. Methods In Molecular Biology 2022, 2557: 583-594. PMID: 36512239, DOI: 10.1007/978-1-0716-2639-9_35.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkProtein exportSecretory pathwaySelective hooks (RUSH) systemCell biological approachesProtein of interestPulse-chase experimentsProtein exitProtein sortingExport kineticsCellular homeostasisProtein transportDifferent cargoesLive cellsBiological approachesRUSH systemProteinPathwayTraffickingHomeostasisExportCargoSortingCompartmentsCells
2021
Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Live-cell Vesicle Tracking in the Secretory Pathway Using HeLa Cells.
Pakdel M, Pacheco-Fernandez N, von Blume J. Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Live-cell Vesicle Tracking in the Secretory Pathway Using HeLa Cells. Bio-protocol 2021, 11: e3958. PMID: 33855118, PMCID: PMC8032492, DOI: 10.21769/bioprotoc.3958.Peer-Reviewed Original ResearchProtein traffickingVesicle traffickingSecretory pathwayGolgi vesicle traffickingSelective hooks (RUSH) systemHeLa cellsLive-cell microscopyMammalian cellsProtein cargoLive-cell monitoringCargo vesiclesVesicle trackingTraffickingRUSH systemSorting assaysWide-field microscopyPhysiological conditionsVesiclesMore detail processesPathwayCargoCellsFinal destinationProteinSecretoryRetention Using Selective Hooks (RUSH) Cargo Sorting Assay for Protein Vesicle Tracking in HeLa Cells.
Pacheco-Fernandez N, Pakdel M, Von Blume J. Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Protein Vesicle Tracking in HeLa Cells. Bio-protocol 2021, 11: e3936. PMID: 33796610, PMCID: PMC8005885, DOI: 10.21769/bioprotoc.3936.Peer-Reviewed Original ResearchSecretory pathwaySelective hooks (RUSH) systemProtein sortingVesicle traffickingSpecific cargoCargo traffickingProtein cargoTrafficking dynamicsProtein dynamicsVesicle trackingBiotin additionHeLa cellsFluorescent vesiclesTraffickingSorting assaysPathwayCargoCellsProteinExcellent toolSuch studiesVesiclesBetter understandingRegulationSorting
2020
Seizure protein 6 controls glycosylation and trafficking of kainate receptor subunits GluK2 and GluK3
Pigoni M, Hsia H, Hartmann J, Rudan Njavro J, Shmueli MD, Müller SA, Güner G, Tüshaus J, Kuhn P, Kumar R, Gao P, Tran ML, Ramazanov B, Blank B, Hipgrave Ederveen A, Von Blume J, Mulle C, Gunnersen JM, Wuhrer M, Rammes G, Busche MA, Koeglsperger T, Lichtenthaler SF. Seizure protein 6 controls glycosylation and trafficking of kainate receptor subunits GluK2 and GluK3. The EMBO Journal 2020, 39: embj2019103457. PMID: 32567721, PMCID: PMC7396870, DOI: 10.15252/embj.2019103457.Peer-Reviewed Original ResearchConceptsPrimary neuronsCell surface localizationMolecular functionsKainate receptor subunit GluK2Trafficking factorsSecretory pathwayNovel functionHeterologous cellsMajor substrateSurface localizationProtein 6Alzheimer's diseaseCA1 pyramidal neuronsAcute hippocampal slicesProtease BACE1Kainate-evoked currentsGlycosylationGluK2/3Pyramidal neuronsGluK2Hippocampal slicesKainate receptorsPsychiatric disordersNervous systemPsychiatric diseases