2021
Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Protein Vesicle Tracking in HeLa Cells.
Pacheco-Fernandez N, Pakdel M, Von Blume J. Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Protein Vesicle Tracking in HeLa Cells. Bio-protocol 2021, 11: e3936. PMID: 33796610, PMCID: PMC8005885, DOI: 10.21769/bioprotoc.3936.Peer-Reviewed Original ResearchSecretory pathwaySelective hooks (RUSH) systemProtein sortingVesicle traffickingSpecific cargoCargo traffickingProtein cargoTrafficking dynamicsProtein dynamicsVesicle trackingBiotin additionHeLa cellsFluorescent vesiclesTraffickingSorting assaysPathwayCargoCellsProteinExcellent toolSuch studiesVesiclesBetter understandingRegulationSorting
2017
Persistent inhibition of pore-based cell migration by sub-toxic doses of miuraenamide, an actin filament stabilizer
Moser C, Rüdiger D, Förster F, von Blume J, Yu P, Kuster B, Kazmaier U, Vollmar AM, Zahler S. Persistent inhibition of pore-based cell migration by sub-toxic doses of miuraenamide, an actin filament stabilizer. Scientific Reports 2017, 7: 16407. PMID: 29180826, PMCID: PMC5703899, DOI: 10.1038/s41598-017-16759-7.Peer-Reviewed Original ResearchConceptsCell migrationActin-targeting drugsActin-binding compoundsSub-toxic dosesTumor cell migrationProteomic approachSRF signalingNuclear stiffnessActin filament stabilizerProtein clustersActin filamentsH treatmentComplex biologyProtein expressionActinPersistent morphological changesSmall moleculesMiuraenamidesGeneral cytotoxicityNatural compoundsCytotoxic effectsRegulationMorphological changesBoyden chamberMigration
2014
Secretory cargo sorting at the trans-Golgi network
Kienzle C, von Blume J. Secretory cargo sorting at the trans-Golgi network. Trends In Cell Biology 2014, 24: 584-593. PMID: 24841758, DOI: 10.1016/j.tcb.2014.04.007.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkCytoplasmic actin cytoskeletonPlasma membrane deliverySubset of proteinsNovel sorting mechanismKey cellular eventsSecretory cargoActin cytoskeletonCellular eventsBinding proteinLysosomal hydrolasesSorting mechanismProteinNormal physiologySuccessful sortingRecent discoverySortingCab45CytoskeletonHydrolasesSPCA1ATPaseRegulationPhysiologyCargo